Temporal and spatial dynamics of harmful algal bloom-associated microbial communities in eutrophic Clear Lake, California

Applied and Environmental Microbiology, Journal Year: 2025, Volume and Issue: unknown

Published: March 28, 2025

ABSTRACT Clear Lake is a large, natural eutrophic lake located in northern California, United States. Persistent, toxic cyanobacterial blooms have been reported the since 2011. However, our understanding of spatiotemporal distribution toxin-producing genera and their interaction with biotic abiotic environment limited. Moreover, few studies addressed how co-occurring microbial communities respond to these blooms. Using multi-domain 16S/18S rRNA gene amplicon sequencing, strong seasonal succession within eukaryotic assemblage was identified, which primarily explained by variation total phosphorus (~30%, P < 0.001) temperature (~15%, 0.01). Cyanobacterial often initiated proliferation diazotrophs ( Dolichospermum Nodularia ) concomitant increases nitrogen, followed non-diazotrophs, such as Microcystis, Limnothrix, Planktothrix . The picocyanobacterium Cyanobium , previously undocumented lake, dominant summer taxon western part Lake, accounting for ~45%–80% relative abundance reads. Seasonal influenced photosynthetic chlorophytes diatoms, well mixotrophic ciliates cryptophytes. Among all genera, Microcystis most strongly correlated microcystin concentrations 0.001), both appeared influence eukaryotes. Finally, using putative relationships based on correlation sequence environmental variables, several potential grazers were including cyclopoid copepods Cryptomonas These correlations need further confirmation experimental work validate nature relationships. IMPORTANCE an important habitat fish wildlife, also provides myriad human benefits, recreation, irrigation, drinking water. vital tribal tradition cultural practices. last decade, has experienced recurring harmful algal toxin levels that frequently exceed California voluntary guidance levels. high pose substantial threat residents, visitors, sustenance fishing beneficial uses. significant gaps remain bloom dynamics environments. This study characterized spatial patterns bloom-causing cyanobacteria identified major producer Lake. Additionally, bacterial characterized, interactions discussed.

Language: Английский

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Microbial community structure and functional properties in permanently and seasonally flooded areas in Poyang Lake

Scientific Reports, Journal Year: 2020, Volume and Issue: 10(1)

Published: March 16, 2020

Water level fluctuations are an inherent feature regulating the ecological structures and functions of lakes. It is vital to understand effects water on bacterial communities metabolic characteristics in freshwater lakes a changing world. However, information microbial community structure functional properties permanently seasonally flooded areas lacking. Poyang Lake typical seasonal lake linked Yangtze River significantly affected by fluctuations. Bottom was collected from 12 sampling sites: seven inundated for whole year (inundated areas) five drained during dry season (emerged areas). High-throughput 16S rRNA gene sequencing used identify communities. The results showed that taxonomic potential were different between emerged areas. Cyanobacteria dominant both areas, but relative abundance much higher than Bacterial taxonomically sensitive functionally Nitrogen, phosphorus, dissolved organic carbon concentrations their ratios, as well oxygen, played important roles promoting compositional patterns According predictions based sequences, genes related assimilatory nitrate reduction dissimilatory higher. These differences might have been caused nitrogen intra-annual associated with denitrification not This study improved our knowledge processes lake.

Language: Английский

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Interannual and Seasonal Shift between Microcystis and Dolichospermum: A 7-Year Investigation in Lake Chaohu, China

Water, Journal Year: 2020, Volume and Issue: 12(7), P. 1978 - 1978

Published: July 13, 2020

The shifts among bloom-forming cyanobacteria have attracted increasing attention due to the reductions in nitrogen and phosphorus during eutrophication mitigation process. However, knowledge is limited regarding pattern drivers of these cyanobacterial genera. In this study, we performed a 7-year long, monthly investigation Lake Chaohu, analyze interannual seasonal between Microcystis Dolichospermum. Our results showed that was dominant cyanobacterium western lake region summer, whereas Dolichospermum other regions seasons. biomass ratio were driven primarily by total temperature. sensitivity nutrients temperature relatively weak compared Microcystis. might be led If level high, then grew rapidly, competitively excluded nutrient level, especially low, exclusive power weak, maintained its dominance, even summer. key (~17 °C) determined dominance two cyanobacteria. never dominated, while always below had different means responding interaction temperature, phosphorus. sensitive variation depended on While nitrogen. ways contribute succession findings will helpful for improving understanding shift process

Language: Английский

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Fate of Planktothrix-derived toxins in aquatic food webs: A case study in Lake Mindelsee (Germany)

Ecotoxicology and Environmental Safety, Journal Year: 2024, Volume and Issue: 273, P. 116154 - 116154

Published: Feb. 28, 2024

Blooms of the red, filamentous cyanobacterium Planktothrix rubescens occur frequently in pre-alpine lakes Europe, often with concomitant toxic microcystin (MC) production. Trophic transfer MCs has been observed bivalves, fish, and zooplankton species, while uptake into Diptera species could facilitate distribution terrestrial food webs habitats. In this study, we characterized a bloom summer 2019 Lake Mindelsee tracked possible trophic and/or bioaccumulation via analysis phytoplankton, (Daphnia) emergent aquatic insects (Chaoborus, Chironomidae Trichoptera). Using 16 S rRNA gene amplicon sequencing, found that five sequence variants spp. were responsible for formation September October 2019, these MC-producing variants, provisionally identified as P. isothrix serta, occurred exclusively (Germany), other also detected nearby Constance. The remaining cyanobacterial community was dominated by Cyanobiaceae high overlap Constance, suggesting well-established exchange cyanobacteria between adjacent lakes. With targeted LC-HRMS/MS two MC-congeners, MC-LR [Asp

Language: Английский

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Limited response of a spring bloom community inoculated with filamentous cyanobacteria to elevated temperature and pCO₂

Botanica Marina, Journal Year: 2018, Volume and Issue: 62(1), P. 3 - 16

Published: Nov. 9, 2018

Abstract Temperature and CO 2 levels are projected to increase in the future, with consequences for carbon nutrient cycling brackish environments, such as Baltic Sea. Moreover, filamentous cyanobacteria predicted be favored over other phytoplankton groups under these conditions. Under a 12-day outdoor experiment, we examined effect on natural spring bloom community of elevated temperature (from 1°C 4°C) p 390 970 μatm). No effects or were observed biovolumes, but significantly higher photosystem II activity was at after 9 days. In addition, three species diazotrophic inoculated test their competitive capacity The toxic cyanobacterium Nodularia spumigena exhibited an average specific growth rate 0.10 d −1 by end indicating potential prevalence even during wintertime Generally, none able outcompete temperatures ≤4°C. direct found heterotrophic bacteria. This study demonstrates highly efficient resistance towards short-term (12 days) changes abiotic factors Sea community.

Language: Английский

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Community Structure and Toxicity Potential of Cyanobacteria during Summer and Winter in a Temperate-Zone Lake Susceptible to Phytoplankton Blooms

Toxins, Journal Year: 2024, Volume and Issue: 16(8), P. 357 - 357

Published: Aug. 14, 2024

Cyanobacterial blooms are increasingly common during winters, especially when they mild. The goal of this study was to determine the summer and winter phytoplankton community structure, cyanotoxin presence, toxigenicity in a eutrophic lake susceptible cyanobacterial throughout year, using classical microscopy, an analysis toxic cyanometabolites, genes involved biosynthesis cyanotoxins. We also assessed whether diversity studied has changed compared what reported previous reports conducted several years ago. Moreover, bloom-forming strains were isolated from screened for presence toxigenicity. Cyanobacteria main component both sampling times, and, particular, Oscillatoriales predominant (

Language: Английский

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Cyanobacteria in winter: seasonal dynamics of harmful algal blooms and their driving factors in boreal lakes

Heliyon, Journal Year: 2024, Volume and Issue: 10(24), P. e40687 - e40687

Published: Nov. 30, 2024

Language: Английский

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Early and late cyanobacterial bloomers in a shallow, eutrophic lake

Environmental Science Processes & Impacts, Journal Year: 2022, Volume and Issue: 24(8), P. 1212 - 1227

Published: Jan. 1, 2022

Cyanobacterial blooms present challenges for water treatment, especially in regions like the Canadian prairies where poor quality intensifies treatment issues. Buoyant cyanobacteria that resist sedimentation a challenge as operators attempt to balance pre-treatment and toxic disinfection by-products. Here, we used microscopy identify describe succession of cyanobacterial species Buffalo Pound Lake, key drinking supply. We indicator analysis temporal grouping structures throughout two sampling seasons from May October 2018 2019. Our findings highlight bloom phases - mid-summer diazotrophic Dolichospermum spp. an autumn Planktothrix agardhii bloom. crassa Woronichinia compacta served indicators phases, respectively. Different metabolites were associated with distinct both years: microcystins some newly monitored cyanopeptides (anabaenopeptin A B) Despite forming significant proportion phytoplankton biomass (>60%), had previously not been detected by sensor or laboratory-derived chlorophyll-a. results demonstrate power targeted taxonomic identification tool managers bloom-prone systems. Moreover, has potential disrupt due its evasion detection. importance identifying this given expectation warmer temperatures longer ice-free season will become norm.

Language: Английский

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Combining Imaging Flow Cytometry and Molecular Biological Methods to Reveal Presence of Potentially Toxic Algae at the Ural River in Kazakhstan

Frontiers in Marine Science, Journal Year: 2021, Volume and Issue: 8

Published: July 21, 2021

Algal blooms occur in freshwater bodies throughout the world, often leading to fish kills. Cases of these kills along Ural River were reported 2018–2019, involving significant amount sturgeon farming areas. In this study, analysis algal samples from delta up 100 km inland was carried out August December 2019 using imaging flow cytometry (IFC), molecular biological, and microscopic techniques. We identified filamentous cyanobacteria Cuspidothrix issatschenkoi , Dolichospermum cf. flos-aquae macrosporum Pseudanabaena limnetica Planktothrix spp. as dominant potentially toxic phytoplankton species, we also found minor quantities Cylindrospermopsis raciborskii . For first time, phylogenetic investigations field clones establish taxa species identify presence genes encoding toxins. The complementary with nanopore-based next-generation sequencing overlapped results IFC instrumental revealing taxa. Real-time PCR indicated Microcystis ADA-clade well associated production microcystin ( mcyE ) neurotoxin saxitoxin sxtA originating cyanobacteria. These findings suggest that toxin-producing could become a threat near Atyrau, which can significantly affect aquaculture region.

Language: Английский

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Antibiotic-Induced Changes in Pigment Accumulation, Photosystem II, and Membrane Permeability in a Model Cyanobacterium

Frontiers in Microbiology, Journal Year: 2022, Volume and Issue: 13

Published: June 22, 2022

Fremyella diplosiphon is a well-studied model cyanobacterium for photosynthesis due to its efficient light absorption potential and pigment accumulation. In the present study, impact of ampicillin, tetracycline, kanamycin, cefotaxime on fluorescence photosynthetic capacity in strains B481-WT B481-SD was investigated. Our results indicated that both exposed kanamycin from 0.2 3.2 mg/L tetracycline 0.8 12.8 enhanced growth Additionally, treated with 0.2–51.2 ampicillin resulted significant enhancement fluorescence. A detrimental effect pigmentation 6.4–102.5 0.8–102.5 observed. Detection reactive oxygen species revealed highest levels oxidative stress at 51.2 102.5 B481- SD B481-WT. Membrane permeability detected by lactate dehydrogenase assay maximal activity treatments day 6. Abundant vacuolation, pyrophosphate, cyanophycin granule formation were observed cells as response antibiotic stress. These findings hormetic antibiotics F. indicate optimal concentrations induce cellular while high severely functionality. Future studies will be aimed enhance lipid productivity disintegrate cell wall, thus paving way clean bioenergy applications.

Language: Английский

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