Shark feeding affects health and female reproductive investment in blacktip reef sharks from French Polynesia DOI Open Access
Suzanne C. Mills, Serge Planes, Johann Mourier

et al.

Animal Conservation, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 24, 2024

Abstract Ecotourism is branded as transforming wildlife biodiversity conservation; yet, its positive and negative effects are not always assessed in wild populations. Wildlife viewing with feeding a popular form of ecotourism, but potential health impacts on becoming increasingly evident. Shark global phenomenon; however, impact studies species' persistence (i.e. survival reproduction) lacking. In this study, we expand upon previous work shark tourism use physiological indicators to assess the sub‐lethal fitness consequences feeding. Blood cellular, biochemical endocrinological parameters were sampled from 117 adult blacktip reef sharks, Carcharhinus melanopterus , at non‐feeding sites around Mo'orea, French Polynesia, compare general condition, nutritional status, metabolism proxy reproductive investment respective sites. addition sex‐ season‐specific differences observed multiple parameters, found lower haematocrit levels (condition) for both sexes, well insulin (metabolism proxy) male sharks Further physiology interaction breeding season: females using had glucose (nutritional status), 17β‐oestradiol during season (reproductive investment) compared Male exhibited higher testosterone Our results suggest that activity Mo'orea provides poor nutrition and/or unpredictable food, especially site‐attached energy‐intensive season. We highlight how reveal feeding, potentially having longer‐lasting population dynamics, making an ecological trap. Animal sex should be considered when evaluating impacts, stricter regulations content food given needed ecotourism management.

Language: Английский

Partial Replacement of Fish Meal with an Aquatic Macrophyte, Ceratophyllum demersum in the Diet of Common Carp, Cyprinus carpio var. communis Fingerlings DOI Creative Commons
Imtiaz Ahmed, Anzar Lateef, Kousar Jan

et al.

Aquaculture Research, Journal Year: 2024, Volume and Issue: 2024(1)

Published: Jan. 1, 2024

A 12‐week feeding trial was conducted to investigate the nutrient utilization, growth performance, and hematological indices of Cyprinus carpio var. communis fingerlings fed diets based on aquatic macrophyte, Ceratophyllum demersum as a replacement fish meal (FM). Six isonitrogenous isocaloric containing graded levels C. 0%, 5%, 10%, 15%, 20%, 25%, respectively, replacer FM were formulated. Total 360 with an initial weight 3.65 ± 0.98 g randomly stocked in 70 L plastic tanks water volume 60 connected continuous flow‐through system (1–1.5 L/min) for each treatment run triplicate having 20 tank. At end trial, it observed that receiving 5% 10% diet showed improvements live gain (LWG), specific rate (SGR), feed conversion ratio (FCR), protein efficiency (PER). While further addition from 15% 25% led progressive decline values LWG, SGR, FCR, PER. Hematological data also exhibited linear declining trend beyond level. The higher inclusions significantly ( P < 0.05) affected whole body composition lowest fat amounts recorded at 0.05). highest contents diets. Except serum glucose, cholesterol, alanine aminotransferase (ALT), aspartate (AST), other decreasing increasing inclusion diet. results present study clearly demonstrated did not affect parameters compared 50% However, is recommended can be substituted without compromising nutritional quality fish.

Language: Английский

Citations

4
Preliminary Evaluation of an Automated Blood Cell Analyzer for Its Use with Blood Samples from Rainbow Trout Oncorhynchus mykiss DOI Creative Commons
Montse Mesalles,

Meritxell Uroz,

I. Brandts

et al.

Animals, Journal Year: 2025, Volume and Issue: 15(9), P. 1265 - 1265

Published: April 29, 2025

Hematological studies provide essential information about the health of animals, which is crucial for veterinary medicine, scientific research, and aquaculture. Automatic hematological analyzers are an alternative to manual methods, offering faster more reliable results. The objective this study was validate Sysmex XN-1000V automatic hematology analyzer blood samples from rainbow trout (Oncorhynchus mykiss), examine effects two anticoagulants (K2EDTA lithium heparin), establish normal reference values fish species. Additionally, comparative were conducted between methods (hemocytometer cell count smear estimation), intervals established. Ninety-nine heparinized analyzed validation sample stability tests. results showed extremely good precision, with a coefficient variation (CV) below 3% RBCs, HGB, HCT less than 5% non-RBC cells (leukocytes plus thrombocytes). However, heterophils (%) exhibited higher variability, CV 15.08%. Linearity excellent, carry-over 1% all parameters. test indicated that could be up 48 h when stored at 4 °C 24 room temperature. Non-RBC first degrade over time. automated demonstrated correlation agreement, validating analyzer's accuracy. anticoagulants, K2EDTA heparin, on also studied. Heparin preferred anticoagulant routine analysis analyzer. In conclusion, enables complete hemogram analyses performed quickly accurately, standardizing techniques, harmonizing results, providing O mykiss blood.

Language: Английский

Citations

0
LC–MS/MS based characterisation and differential expression of proteins in Himalayan snow trout, Schizothorax labiatus using LFQ technique DOI Creative Commons
Kousar Jan, Imtiaz Ahmed, Nazir Ahmad Dar

et al.

Scientific Reports, Journal Year: 2023, Volume and Issue: 13(1)

Published: June 22, 2023

Molecular characterization of fish muscle proteins are nowadays considered as a key component to understand the role specific involved in various physiological and metabolic processes including their up down regulation organisms. Coldwater specimens snow trouts hold different types which help them survive highly diversified temperatures fluctuating from 0 20 °C. So, current study, liquid chromatography mass spectrometry using label free quantification technique has been used investigate proteome profile Schizothorax labiatus. For proteomic two weight groups S. labiatus were taken river Sindh. The analysis group 1 revealed that total 235 male 238 female recorded. However, when compared with each other on basis spectral count abundance peptides by ProteinLynx Global Server software, 14 down-regulated 22 up-regulated noted this group. ones included homeodomain protein HoxA2b, retinol-binding 4, MHC class II beta chain proopiomelanocortin while expressed comprised gonadotropin I subunit, NADH dehydrogenase subunit manganese superoxide dismutase, recombinase-activating 2, glycosyltransferase, chymotrypsin cytochrome b. On hand, characterisation 2 227 194 When quantification, 18 expression hepatic lipase, allograft inflammatory factor-1, 4 myostatin glycogen synthase kinase-3 variant 5, cholecystokinin, 3 Significant (P < 0.05) difference was also between sexes According MS analysis, primarily concerned growth, skeletal development metabolism Sindh, indicates growth during season collection i.e., winter slow owing less food availability, gonad low activity. While, related immune response be thereby signifying ecosystem pollution loads, microbial, pathogenic anthropogenic activities. It found metabolism, reproductive processes, particularly lipid significant these may connected pre-spawning, use stored source energy. information generated study can applied future research aimed at enhancing traceability, safety, risk management authenticity analysis.

Language: Английский

Citations

3
Towards a comprehensive understanding of the muscle proteome in Schizothorax labiatus: Insights from seasonal variations, metabolic responses, and reproductive signatures in the River Jhelum DOI
Kousar Jan, Imtiaz Ahmed, Nazir Ahmad Dar

et al.

The Science of The Total Environment, Journal Year: 2024, Volume and Issue: 919, P. 170840 - 170840

Published: Feb. 8, 2024

Language: Английский

Citations

0
Shark feeding affects health and female reproductive investment in blacktip reef sharks from French Polynesia DOI Open Access
Suzanne C. Mills, Serge Planes, Johann Mourier

et al.

Animal Conservation, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 24, 2024

Abstract Ecotourism is branded as transforming wildlife biodiversity conservation; yet, its positive and negative effects are not always assessed in wild populations. Wildlife viewing with feeding a popular form of ecotourism, but potential health impacts on becoming increasingly evident. Shark global phenomenon; however, impact studies species' persistence (i.e. survival reproduction) lacking. In this study, we expand upon previous work shark tourism use physiological indicators to assess the sub‐lethal fitness consequences feeding. Blood cellular, biochemical endocrinological parameters were sampled from 117 adult blacktip reef sharks, Carcharhinus melanopterus , at non‐feeding sites around Mo'orea, French Polynesia, compare general condition, nutritional status, metabolism proxy reproductive investment respective sites. addition sex‐ season‐specific differences observed multiple parameters, found lower haematocrit levels (condition) for both sexes, well insulin (metabolism proxy) male sharks Further physiology interaction breeding season: females using had glucose (nutritional status), 17β‐oestradiol during season (reproductive investment) compared Male exhibited higher testosterone Our results suggest that activity Mo'orea provides poor nutrition and/or unpredictable food, especially site‐attached energy‐intensive season. We highlight how reveal feeding, potentially having longer‐lasting population dynamics, making an ecological trap. Animal sex should be considered when evaluating impacts, stricter regulations content food given needed ecotourism management.

Language: Английский

Citations

0