Revealing Genetic Diversity and Population Structure in Türkiye’s Wheat Germplasm Using iPBS-Retrotransposon Markers

Agronomy, Journal Year: 2024, Volume and Issue: 14(2), P. 300 - 300

Published: Jan. 30, 2024

Investigating the genetic diversity and population structure of wheat germplasm is crucial for understanding underlying variability essential breeding programs preservation. This research aims to contribute novel insights with respect makeup relationships among these genotypes, shedding light on present within Turkish germplasm. In this study, iPBS-retrotransposon markers were employed analyze 58 encompassing 54 landraces 4 cultivars sourced from Türkiye. These serve as indicators that can be used evaluate variation, build genealogical trees, comprehend evolutionary connections. The PCR products visualized agarose gel, bands scored present/absent. ten iPBS primers collectively yielded an average 16.3 alleles, generating a total 163 polymorphic bands. number alleles produced by individual ranged (iPBS-2386) 29 (iPBS-2219). parameters calculated using popgen powermarker programs. structures assessed ntsys Polymorphism information content (PIC) per marker varied 0.13 (iPBS-2390) 0.29 (iPBS-2386), value 0.22. Shannon’s index (I) was 1.48, while effective (Ne) Nei’s (H) determined 0.26 0.31, respectively. Genotype numbers 3 (Triticum dicoccum) 10 monococcum) exhibited maximum distance 0.1292, signifying highest disparity. Population analysis revealed segregation genotypes into three distinct subpopulations. Notably, substantial portion clustered populations correlated species. result consistent categorization based comprehensive assessment noteworthy allele distribution, polymorphism content, differentiation, offering valuable implications strategies conservation efforts. addition, in study hold significant potential applications

Language: Английский

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Genome assembly and SSR molecular marker development and utilization in Sapindus mukorossi

Industrial Crops and Products, Journal Year: 2025, Volume and Issue: 226, P. 120694 - 120694

Published: Feb. 17, 2025

Language: Английский

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Natural Diversity of Crataegus monogyna Jacq. in Northeastern Türkiye Encompassing Morphological, Biochemical, and Molecular Features

Horticulturae, Journal Year: 2025, Volume and Issue: 11(3), P. 238 - 238

Published: Feb. 24, 2025

Hawthorn (Cratageus monogyna Jacq.), one of the important wild fruit species in Turkey, is an many countries due to its use traditional medicine, landscaping studies, and food beverage industry. In this study, morphological characterization revealed significant variation among genotypes, with weight ranging from 0.55 g 2.87 g, width 9.74 mm 18.39 mm, leaf 19.53 41.19 mm. Genotype-dependent variations were observed total phenolic content (151.34 mg–491.88 mg GAE 100 g−1 flesh), flavonoid (16.74–48.13 CAE anthocyanin (26.34–79.79 cyn-3 gluc antioxidant activity (29.20% 56.70%). HPLC-based profiling identified substantial key bioactive compounds, highest levels chlorogenic acid (16.16 fw), caffeic (10.21 epicatechin (13.83 rutin (74.05 protocatechuic (2.00 fw). ISSR marker-based molecular analysis a high degree genetic polymorphism (89.12%), 55 out 62 bands classified as polymorphic. The polymorphic information values ranged 0.34 0.44. Jaccard similarity coefficient 0.04 (M9 M16) 0.63 (M17 M3), indicating variability. tanglegram comparing morphological–biochemical dendrograms yielded entanglement score 0.714, alignment between phenotypic data. These findings show that hawthorn genotypes Kelkit Valley exhibit extensive biochemical diversity, which critical for conservation efforts, breeding programs, development high-value medicinal functional products.

Language: Английский

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Evaluating terminal heat stress tolerance and genetic variation in emmer wheat (Triticum dicoccum L.) germplasm using morphological and molecular approaches

Euphytica, Journal Year: 2025, Volume and Issue: 221(5)

Published: April 12, 2025

Language: Английский

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Determination of Genetic Diversity of Some Upland and Sea Island Cotton Genotypes Using High-Resolution Capillary Electrophoresis Gel

Agronomy, Journal Year: 2023, Volume and Issue: 13(9), P. 2407 - 2407

Published: Sept. 18, 2023

Cotton is a major source of natural fibre for the global textile industry and also an important oilseed crop. main textiles, seeds are used oil remaining bagasse as high-protein animal feed. In addition, cotton’s so-called short in more than 50 industries. breeding generally based on crossing best yielding quality genotypes. However, cotton programmes negatively affected by narrow genetic diversity varieties. It this reason that identification resources disclosure so important. Here, G. hirsutum barbadense genotypes was determined using high-resolution capillary gel electrophoresis. Using 19 EST-SSR markers, total 47 were screened. The PIC values markers ranged from 0.268 to 0.889. mean value calculated be 0.603. terms clustering, PCoA population structure analyses gave similar results, could divided into three groups. Genetic admixture with found some species. We can conclude (i) study effective determination diversity, (ii) should increased through collection (iii) considered them QTL studies.

Language: Английский

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Genetic Diversity of Some Upland and Sea Island Cotton Genotypes by High Resolution Capillary Electrophoresis Gel

Published: Aug. 21, 2023

Cotton (Gossypium L.) is a major source of natural fibre for the world's textile industry and also an important oil plant. main textiles, seeds are used rest bagasse high-protein animal feed. In addition, more than 50 industries use cotton's so-called short fibre. Breeding studies in cotton generally based on crosses between best genotypes terms yield quality. However, narrow genetic diversity cultivars has negative impact breeding programmes. For this reason, identification resources disclosure important. Herein, high-resolution capillary gel electrophoresis was to determine G. hirsutum barbadense genotypes. Using 19 EST-SSR markers, total 47 were screened. The PIC values markers ranged from 0.268 0.889. mean value calculated be 0.603. Concerning clustering, PCoA population structure analyses gave similar results, could divided into 3 groups. Genetic admixture with found some species. We can conclude that i) study effective determination diversity, ii) should increased through collection iii) genic considered programmes by using them QTL studies.

Language: Английский

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Abundant Genetic Diversity Harbored by Traditional Naked Barley Varieties on Tibetan Plateau: Implications in Their Effective Conservation and Utilization

Biology, Journal Year: 2024, Volume and Issue: 13(12), P. 1018 - 1018

Published: Dec. 5, 2024

Naked barley (Hordeum vulgare var. nudum) is a staple food crop, contributing significantly to global security. Understanding genetic diversity will facilitate its effective conservation and utilization. To determine distribution within among varieties, we characterized 30 naked varieties from Tibet, representing the traditional, modern, germplasm-resources-bank gene pools, by analyzing SSR molecular fingerprints. The results demonstrate abundant in Tibetan particularly those traditional pool that holds much more private (unique) alleles. Principal coordinates STRUCTURE analyses indicate substantial deviation of modern varieties. A considerable amount seed mixture detected suggesting practices using mixed seeds modern-variety cultivation. Cluster further narrow background likely due limited number traditional/germplasm-resources-bank applied breeding. Relationships between increases sample sizes highlight importance sampling strategies for field collections. findings this study have important implications sustainable utilization different types germplasm, both Tibet other regions around world.

Language: Английский

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