Biodiversity Evolution of a Shrub Betula nana L. Populations in the Urals

International Journal of Forestry Research, Journal Year: 2024, Volume and Issue: 2024(1)

Published: Jan. 1, 2024

Genetic polymorphism of B. nana along the Ural ridge is analyzed based on variability trn S -trn G, L F, T, K1 K2, and mat cpDNA markers with application RFLP method. Specimens pubescens were additionally taken for comparative analysis. The research revealed 12 chlorotypes in populations, among which A B most frequent. Polar Urals populations are characterized by prevailing chlorotype A, significantly decreased genetic (He = 0.169), low divergence (Fst 0.000–0.050), possibly associated Pleistocene Holocene migration processes, edge effect, specific ecological conditions arctic tundra. Middle show higher 0.296). Reduced gene flow between bog results high interpopulation (Gst 0.701, Nst 0.764). Shared haplotypes interspecific differentiation indicated (Gct 0.007, Nct 0.064). Flow cytometry homogeneous DNA content specimen (Serebryansky Kamen Sredneuralsk) a total 2C variation range 0.91–1.09 pg 1.85–2.10 absence triploid hybrids studied samples, suggests frequency occurrence latter and, possibly, northern localization these birch species hybridization zones.

Language: Английский

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DNA barcoding, inter-specific and inter-generic variation studies on South Indian scaly tree ferns ( Cyathea sp.pl.) using chloroplast rbc L gene

Plant Biosystems - An International Journal Dealing with all Aspects of Plant Biology, Journal Year: 2024, Volume and Issue: 158(5), P. 990 - 1005

Published: Aug. 7, 2024

DNA barcoding is an effective identification tool for tree ferns with heteromorphic generations morphologically simple gametophytic phase. A complete reference dataset which includes coverage of the target local flora necessary accurate identification. Three different Cyathea sp.pl. were investigated to test utility plastid barcode regions rbcL intention developing system native pteridophytes. In addition, inter-specific and inter-generic variations among also focused. BLAST analysis revealed similar sequences related C. nilgirensis Holttum, gigantea (Wallich ex Hook. f.) Holttum crinita (Hook.) Copel a sum 143 viz., (121), Gymnosphaera sp.pl.(4), Sphaeropteris (1) Alsophila (17). The plants retrieved from GenBank in FASTA format. aligned using multiple sequence alignment MUSCLE. distance percentage calculated Maximum Likelihood, Minimum Evolution, UPGMA (Unweighted Pair Group Method Arithmetic Mean) Neighbor-Joining method. analyses constructed MEGA X software. gene provided variation its strong resolving power. This study demonstrates overall effectiveness barcodes species fern genus pteridophyte Southern Western Ghats.

Language: Английский

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Phylogenetic study of Iris plants in China based on chloroplast matK gene and nuclear ITS gene

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 30, 2024

Using both Neighbor-Joining and Maximum Likelihood algorithms, the ITS matK sequences of Iris species in China were sequenced to explore phylogenetic relationships six subgenera China. After alignment sequences, a total 893 characters obtained for sequence, with length variation range 704–824 bp 890 865–887 bp. The clustering analysis results generally consistent subgenus classification Results suggested Subgen. has relatively complex relationship within genus, further research is needed refine internal classifications. Limniris resolved as polyphyletic are quite complex. Nepalensis natural taxonomic group. Crossiris an unnatural It recommended remove Xyridion from system redefine Belamcanda chinensis Pardanthopsis genus.

Language: Английский

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Identification and Genetic Diversity Analysis of Cinnamomum parthenoxylon (Jack) Meisn Species in Song Hinh Protection Forest, Vietnam Based on Three Chloroplast Gene Regions

Biomedical and Biotechnology Research Journal (BBRJ), Journal Year: 2024, Volume and Issue: 8(4), P. 415 - 421

Published: Oct. 1, 2024

Abstract Background: Cinnamomum parthenoxylon is an important component of tropical Vietnam and has major ecological economic significance, which been threatened in recent decades due to the destruction its habitat overexploitation. Methods: In present study, first, three chloroplast DNA (cpDNA) regions ( matK, rbcL , trnH-psbA ) were initially examined identify C. species using Maximum Likelihood (ML). Then, genetic diversity analysis Song Hinh protection forest, Phu Yen Province, Vietnam. Results: The nucleotide sequences determined be 786bp, 513bp, 464bp, respectively. novel have deposited Genbank with matK (PQ106670-PQ106689), (PQ106690-PQ106709), (PQ1066710-PQ106729). revealed that mean base compositions T (34.8%, 28.3%, 30.4%), C (19.4%, 22.5%, 15.1%), A (29.1%, 26.6%, 39.8%), G (16.7%, 22.7%, 14.7%), each cpDNA regions, GC content was discovered significantly lower than AT content. According ML phylogenetic analyses, all samples forest are closely related GenBank, robust supporting values. Moreover, only 0.25% (2 haplotypes) showed polymorphism region, whereas 0.78% (4 region. most variable (3.44%) (16 intergenic region useful . variations moderate, average number differences (Kt = 1.042–4.984), haplotype (Hd 0.521–0.916), (Pi 1.33–10.74 × 10–3). Conclusions: results this study potential provide valuable information essential for classification, identification plant’s origin, propagation, conservation program

Language: Английский

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DNA barcoding of epiphytic orchids for species level identification

The Journal of Horticultural Science and Biotechnology, Journal Year: 2024, Volume and Issue: unknown, P. 1 - 11

Published: July 12, 2024

Language: Английский

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Assessment of Genetic Variation and Evolutionary Dynamics in Saffron Through matK and rbcL barcodes

Journal of Applied Research on Medicinal and Aromatic Plants, Journal Year: 2024, Volume and Issue: unknown, P. 100620 - 100620

Published: Dec. 1, 2024

Language: Английский

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