The interaction networks of small rubber particle proteins in the latex of Taraxacum koksaghyz reveal diverse functions in stress responses and secondary metabolism DOI Creative Commons

Silva Melissa Wolters,

Natalie Laibach,

Jenny Riekötter

et al.

Frontiers in Plant Science, Journal Year: 2024, Volume and Issue: 15

Published: Dec. 13, 2024

The Russian dandelion ( Taraxacum koksaghyz ) is a promising source of natural rubber (NR). synthesis NR takes place on the surface organelles known as particles, which are found in latex – cytoplasm specialized cells laticifers. As well enzymes directly responsible for synthesis, particles also contain small particle proteins (SRPPs), most abundant SRPP3, 4 and 5. These three support by maintaining stability. We used homology-based searches to identify whole TkSRPP gene family qPCR create their spatial expression profiles. Affinity enrichment-mass spectrometry was applied TkSRPP3/4/5 protein interaction partners T. selected were analyzed using qPCR, confocal laser scanning microscopy heterologous yeast. identified 17 SRPP-like sequences genome, including apparent pseudogenes, 10 paralogs arranged an inverted repeat cluster with , one separate TkSRPP6 ). Their sequence diversity different profiles indicated distinct functions interactomes obtained suggested that TkSRPP4 promiscuous hub binds many from compartments, whereas TkSRPP3 5 have more focused interactomes. Two interactors shared (TkSRPP6 TkUGT80B1) chosen independent validation detailed characterization. TkUGT80B1 triterpenoid glycosylating activity provided first evidence saponin latex. Based its partners, appears play special role endoplasmic reticulum, interacting lipidmodifying may facilitate formation. TkSRPP5 be involved GTPase-dependent signaling act part kinase cascade, roles stress tolerance. draws new connection between TkSRPPs Our data contribute functional differentiation demonstrate unexpected interactions will help further elucidate network linking TkSRPPs, responses biosynthesis within cellular complexity

Language: Английский

Identification and characterization of Phaseolus vulgaris CHS genes in response to salt and drought stress DOI Creative Commons
Murat Isıyel, Emre İlhan, Ayşe Gül Kasapoğlu

et al.

Genetic Resources and Crop Evolution, Journal Year: 2024, Volume and Issue: unknown

Published: April 17, 2024

Abstract Common beans hold significant importance in sustainable agriculture and their critical role human nutrition cannot be overstated. Factors such as climate change underscore the necessity for expanding genetic diversity delineating attributes of local bean cultivars. Among various abiotic stressors, drought emerges a formidable constraint limiting cultivation. While diverse set strategies are employed to mitigate impacts stress, ultimate enduring solution lies development drought-resistant cultivars, it can stated that same situation is valid salinity stress. Flavonoid biosynthesis very important plant growth flavonoids known involved many pathways including stress response. This study aimed comprehensively identify characterize CHS gene family within different cultivars exposed salt utilizing genome-wide analysis, assessing expression levels. Employing spectrum silico methodologies, 14 genes were identified common genome. These exhibited molecular weights ranging from 37.38 43.34 kDa consisted 341–393 amino acid residues. Remarkably, all Pvul-CHS shared structure comprising two exons. Phylogenetic analyses conducted across Phaseolus vulgaris , Arabidopsis thaliana Glycine max revealed could classified into three primary clusters. The profiles unveiled varied tissue-specific expressions pivotal roles responding stresses. Furthermore, we assessments chromosomal positions members beans, orthologous associations with related genomes, cis -acting element analyses. outcomes this potential significantly contribute breeding endowed enhanced resilience against conditions.

Language: Английский

Citations

6
The NADPH Oxidases (NOX) Gene Family Expression and Genome-Wide Characterization in Common Beans (Phaseolus vulgaris L.) DOI Creative Commons
A. Eren

Türkiye Tarımsal Araştırmalar Dergisi, Journal Year: 2025, Volume and Issue: 12(1), P. 9 - 17

Published: March 28, 2025

This study aimed to identify and characterize the NADPH oxidases (NOX) gene family in common bean (Phaseolus vulgaris L.) understand its role plant growth, development, stress responses. Using bioinformatic tools, NOX members were identified analyzed for their molecular weights, isoelectric points, amino acid numbers, evolutionary relationships. Segmental duplication analysis phylogenetic studies conducted using genes from Arabidopsis thaliana, Cicer arietinum, Oryza sativa, Glycine max. The results revealed nine Phvul-NOX proteins genome, with weights ranging 92940.09 105660.24 kDa, points 7.86 9.36, numbers 823 946. was observed Phvul-NOX-1/Phvul-NOX-3, Phvul-NOX-2/Phvul-NOX-8, Phvul-NOX-5/Phvul-NOX-6 pairs, purifying selection throughout process. Phylogenetic grouped into three main clades, a synteny map between A. thaliana P. constructed. provides first comprehensive characterization of bean, offering valuable insights future functional genomics research potential applications enhancing tolerance crop productivity.

Language: Английский

Citations

0
Epigenetic Evaluation of Melatonin Application in Bean (Phaseolus vulgaris L.) Genotypes Under Drought and Salt Stress Conditions DOI
Recep AYDINYURT,

Semra Yağcı,

Esra Yaprak

et al.

Plant Molecular Biology Reporter, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 24, 2025

Language: Английский

Citations

0
Genome-wide characterization of the PCO (Plant Cysteine ​​Oxidase) gene family in Phaseolus vulgaris L. and expression analysis under heavy metal stress DOI
Esma Yiğider

South African Journal of Botany, Journal Year: 2025, Volume and Issue: 179, P. 246 - 260

Published: Feb. 22, 2025

Language: Английский

Citations

0
Investigating TIFY Genes for Salt Stress Adaptation in Quinoa (Chenopodium quinoa Willd.): A Genome-Wide Approach DOI Open Access
Esma Yiğider

Erzincan Üniversitesi Fen Bilimleri Enstitüsü Dergisi, Journal Year: 2025, Volume and Issue: 18(1), P. 213 - 228

Published: March 26, 2025

TIFY ailesi, TIFY, Jas ve GATA motiflerini içeren bitkilerin savunma mekanizmalarında stres faktörlerine karşı verdikleri yanıtta önemli rol oynayan bir gen ailesidir. ailesi birçok bitki türünde araştırılmış olmasına rağmen, kinoada henüz incelenmemiştir. Bu çalışmada, 16 Cq-TIFY geni tanımlanmış, bu genler Cq-TIFY-1’den Cq-TIFY-16’ya kadar numaralandırılarak yapısal işlevsel özellikleri karakterize edilmiştir. Tanımlanan proteinlerinin moleküler ağırlıkları 19,99 ile 48,59 kDa, amino asit sayıları 189 450, teorik izoelektrik noktaları ise 4,84 10,1 arasında değişmektedir. Filogenetik analiz sonuçlarına göre, genlerinin üç sınıfa ayrıldığı belirlenmiştir. Gen ailesinin farklı sınıflarındaki üyelerin yapılarının genellikle benzer olduğu Kinoa’da yedi segmental duplikasyon geçirmiş tanımlanmış olup, Ka/Ks analizi genlerin evrimsel süreçte arındırıcı (negatif) seçilime maruz kaldığını göstermiştir. Chenopodium quinoa, Arabidopsis thaliana Spinacia oleracea türleri arasındaki sinteni analizi, genleri açısından ilişki olduğunu ortaya koymuştur. Promotör sonucunda, genlerinde strese duyarlı hormonla ilişkili cis-elementlerin varlığı çıkarılmıştır. Araştırmada, RNA-seq verileri, tuz koşulları altında kök sürgün dokularında ifade modellerini incelemek için kullanılmıştır. Genlerin stresi altındaki profili köklerde sürgünlerde dokuya özgü olarak farklılık göstermiş ifadelerinde anlamlı artış sonuç, toleransı oynayabileceğini düşündürmüştür. çalışma, kinoadaki ailesine dair bilgimizi artırmakta klasik ıslah veya genetik mühendisliği yoluyla toleransını artırmaya yönelik temel oluşturmaktadır.

Citations

0
Integrative multi-omics analysis reveals the potential mechanism by which Streptomyces pactum Act12 enhances wheat root drought tolerance by coordinating phytohormones and metabolic pathways DOI Creative Commons
Jinhui Zhang,

Hongwei Wen,

Shanshan Wang

et al.

BMC Plant Biology, Journal Year: 2025, Volume and Issue: 25(1)

Published: May 27, 2025

Language: Английский

Citations

0
Genome-Wide Analysis of the Nramp Gene Family in Kenaf (Hibiscus cannabinus): Identification, Expression Analysis, and Response to Cadmium Stress DOI Creative Commons

Qin Liu,

Shaocui Li,

Guanghui Du

et al.

Plants, Journal Year: 2024, Volume and Issue: 13(17), P. 2514 - 2514

Published: Sept. 7, 2024

Kenaf (Hibiscus cannabinu) is a grass bast fiber crop that has the ability to tolerate and accumulate heavy metals, it been considered as potential metal accumulator remediation plant. Nramp natural resistance-related macrophage, which plays an important role in transport of divalent ions, plant growth development, abiotic stress. In this study, gene family kenaf was analyzed at whole genome level. A total 15 HcNramp genes were identified. They are distributed unevenly on chromosomes. Phylogenetic analysis classified proteins into 3 different subfamilies. All share specific motif 4 6, belonging same subfamily similar structure motif. The promoters rich hormone response, meristem expression, environmental stress response elements. Under treatments, expression levels vary tissues, most them expressed roots first. These findings can provide basis for understanding cadmium (Cd) stress, great significance screening related Cd tolerance kenaf.

Language: Английский

Citations

1
Chrysin Alleviates Salt Stress in Tomato by Physiological, Biochemical, and Genetic Mechanisms DOI
Melek Ekinci, Metin Turan, Murat Aydın

et al.

Rhizosphere, Journal Year: 2024, Volume and Issue: 32, P. 100979 - 100979

Published: Oct. 25, 2024

Language: Английский

Citations

1
The interaction networks of small rubber particle proteins in the latex of Taraxacum koksaghyz reveal diverse functions in stress responses and secondary metabolism DOI Creative Commons

Silva Melissa Wolters,

Natalie Laibach,

Jenny Riekötter

et al.

Frontiers in Plant Science, Journal Year: 2024, Volume and Issue: 15

Published: Dec. 13, 2024

The Russian dandelion ( Taraxacum koksaghyz ) is a promising source of natural rubber (NR). synthesis NR takes place on the surface organelles known as particles, which are found in latex – cytoplasm specialized cells laticifers. As well enzymes directly responsible for synthesis, particles also contain small particle proteins (SRPPs), most abundant SRPP3, 4 and 5. These three support by maintaining stability. We used homology-based searches to identify whole TkSRPP gene family qPCR create their spatial expression profiles. Affinity enrichment-mass spectrometry was applied TkSRPP3/4/5 protein interaction partners T. selected were analyzed using qPCR, confocal laser scanning microscopy heterologous yeast. identified 17 SRPP-like sequences genome, including apparent pseudogenes, 10 paralogs arranged an inverted repeat cluster with , one separate TkSRPP6 ). Their sequence diversity different profiles indicated distinct functions interactomes obtained suggested that TkSRPP4 promiscuous hub binds many from compartments, whereas TkSRPP3 5 have more focused interactomes. Two interactors shared (TkSRPP6 TkUGT80B1) chosen independent validation detailed characterization. TkUGT80B1 triterpenoid glycosylating activity provided first evidence saponin latex. Based its partners, appears play special role endoplasmic reticulum, interacting lipidmodifying may facilitate formation. TkSRPP5 be involved GTPase-dependent signaling act part kinase cascade, roles stress tolerance. draws new connection between TkSRPPs Our data contribute functional differentiation demonstrate unexpected interactions will help further elucidate network linking TkSRPPs, responses biosynthesis within cellular complexity

Language: Английский

Citations

1