Berberine-loaded iron oxide nanoparticles alleviate cuprizone-induced astrocytic reactivity in a rat model of multiple sclerosis DOI Creative Commons
Ghadha Ibrahim Fouad, Mostafa Mabrouk, Sara A. M. El‐Sayed

et al.

BioMetals, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 14, 2024

Abstract Berberine (BBN) is a naturally occurring alkaloid as secondary metabolite in many plants and exhibits several benefits including neuroprotective activities. However, data on the neuromodulating potential of nanoformulated BBN are still lacking. In present study, loaded within iron oxide nanoparticles (BBN-IONP) were prepared characterized by transmission electron microscopy FTIR, X-ray photoelectron spectroscopy particle-size distribution, zeta potential, HPLC. The remyelinating BBN-IONP relative to free was evaluated against cuprizone (CPZ)-induced neurotoxicity (rats administered 0.2% CPZ powder (w/w) for five weeks). rats treated with either or IONP-BBN (50 mg/kg/day, orally) 14 days. Cognitive function estimated using Y-maze. Biochemically, total antioxidant capacity lipid peroxides reduced glutathione brain tissue, well as, serum interferon-gamma levels estimated. Moreover, genetic expression contents myelin basic protein Matrix metallopeptidase-9 Tumor necrosis factor-α (TNF-α), S100β measured. histopathological patterns immunohistochemical assessment Glial Fibrillary Acidic Protein both cerebral cortex hippocampus CA1 regions investigated. CPZ-rats demonstrated memory restoring, anti-oxidative, anti-inflammatory, anti-astrocytic, Comparing revealed that latter altered activities BBN, showing superior BBN. conclusion, forms possess potential. use IONPs delivery safety these nano-based need further investigation.

Language: Английский

Berberine-loaded iron oxide nanoparticles alleviate cuprizone-induced astrocytic reactivity in a rat model of multiple sclerosis DOI Creative Commons
Ghadha Ibrahim Fouad, Mostafa Mabrouk, Sara A. M. El‐Sayed

et al.

BioMetals, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 14, 2024

Abstract Berberine (BBN) is a naturally occurring alkaloid as secondary metabolite in many plants and exhibits several benefits including neuroprotective activities. However, data on the neuromodulating potential of nanoformulated BBN are still lacking. In present study, loaded within iron oxide nanoparticles (BBN-IONP) were prepared characterized by transmission electron microscopy FTIR, X-ray photoelectron spectroscopy particle-size distribution, zeta potential, HPLC. The remyelinating BBN-IONP relative to free was evaluated against cuprizone (CPZ)-induced neurotoxicity (rats administered 0.2% CPZ powder (w/w) for five weeks). rats treated with either or IONP-BBN (50 mg/kg/day, orally) 14 days. Cognitive function estimated using Y-maze. Biochemically, total antioxidant capacity lipid peroxides reduced glutathione brain tissue, well as, serum interferon-gamma levels estimated. Moreover, genetic expression contents myelin basic protein Matrix metallopeptidase-9 Tumor necrosis factor-α (TNF-α), S100β measured. histopathological patterns immunohistochemical assessment Glial Fibrillary Acidic Protein both cerebral cortex hippocampus CA1 regions investigated. CPZ-rats demonstrated memory restoring, anti-oxidative, anti-inflammatory, anti-astrocytic, Comparing revealed that latter altered activities BBN, showing superior BBN. conclusion, forms possess potential. use IONPs delivery safety these nano-based need further investigation.

Language: Английский

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