Endoplasmic reticulum stress and unfolded protein response: Roles in skeletal muscle atrophy

Biochemical Pharmacology, Journal Year: 2025, Volume and Issue: 234, P. 116799 - 116799

Published: Feb. 12, 2025

Language: Английский

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Hydrogel inspired by "adobe" with antibacterial and antioxidant properties for diabetic wound healing

Materials Today Bio, Journal Year: 2025, Volume and Issue: 31, P. 101477 - 101477

Published: Jan. 10, 2025

With the aging population, incidence of diabetes is increasing. Diabetes often leads to restricted neovascularization, antibiotic-resistant bacterial infections, reduced wound perfusion, and elevated reactive oxygen species, resulting in impaired microenvironments prolonged healing. Hydrogels are important tissue engineering materials for healing, known their high water content good biocompatibility. However, most hydrogels suffer from poor mechanical properties difficulty achieving sustained drug release, hindering clinical application. Inspired by incorporation fibers enhance "adobe," core-shell were introduced into hydrogel. This not only improves strength hydrogel but also enables possibility release. In this study, we first prepared with PLGA (poly(lactic-co-glycolic acid)) PCL (polycaprolactone). was loaded P2 (Parathyroid hormone-related peptides-2), developed our group, which promotes angiogenesis cell proliferation. We then designed a QTG (QCS/TA/Gel, quaternary ammonium chitosan/tannic acid/gelatin) hydrogel, incorporating anti-inflammatory celecoxib exhibits excellent antibacterial biocompatibility, along performance. demonstrates absorption swelling capabilities. early stages can absorb exudate, maintaining stability microenvironment. neovascularization collagen deposition, accelerating healing diabetic wounds, rate exceeding 95 % day 14. Overall, study provides promising strategy developing scaffolds

Language: Английский

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hBMSC-EVs alleviate weightlessness-induced skeletal muscle atrophy by suppressing oxidative stress and inflammation

Stem Cell Research & Therapy, Journal Year: 2025, Volume and Issue: 16(1)

Published: Feb. 4, 2025

Muscle disuse and offloading in microgravity are likely the primary factors mediating spaceflight-induced muscle atrophy, for which there is currently no effective treatment other than exercise. Extracellular vesicles derived from bone marrow mesenchymal stem cells (BMSC-EVs) possess anti-inflammatory antioxidant properties, offering a potential strategy combating weightless muscular atrophy. In this study, human BMSCs-EVs (hBMSC-EVs) were isolated using super-centrifugation characterized. C2C12 myotube nutrition-deprivation mice tail suspension models established. Subsequently, diameter of myotubes, Soleus mass, cross-sectional area (CSA) fibers, grip strength assessed to investigate impact hBMSC-EVs on Immunostaining, transmission electron microscopy observation, western blot analysis employed assess fiber types, ROS levels, inflammation, ubiquitin–proteasome system activity, autophagy lysosome pathway activation skeletal The active can be internalized by myotubes muscle. effectively reduce atrophy caused nutritional deprivation, with concentration 10 × 108 particles/mL showing best effect (P < 0.001). Additionally, down-regulate protein levels associated UPS oxidative stress. Moreover, intravenous administration at 1 1010 reverse reduction soleus mass 0.001), CSA 0.01), 0.001) weightlessness. They demonstrate ability inhibit degradation mediated pathway, along suppression stress inflammatory responses. Furthermore, impede transition slow fibers fast via upregulation Sirt1 PGC-1α levels. Our findings indicate that capable inhibiting excessive suppressing response, reversing type transformation, delaying hindlimb unloading-induced enhancing function. study has further advanced understanding molecular mechanism underlying weightlessness demonstrated protective

Language: Английский

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Manganese-based nanozymes as broad-spectrum antioxidants against cisplatin-induced skeletal muscle atrophy

Chemical Engineering Journal, Journal Year: 2025, Volume and Issue: unknown, P. 159213 - 159213

Published: Jan. 1, 2025

Language: Английский

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Oxidative Stress Regulates the Pathogenesis of Sarcopenia and Its Research Progress on Targeted Intervention of Chinese and Western Drugs

Advances in Clinical Medicine, Journal Year: 2025, Volume and Issue: 15(02), P. 1341 - 1349

Published: Jan. 1, 2025

Language: Английский

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Determination of the stability of a nonsteroidal anti-inflammatory drug for dogs based on celecoxib

Ukrainian Journal of Veterinary and Agricultural Sciences, Journal Year: 2025, Volume and Issue: 8(1), P. 44 - 48

Published: April 23, 2025

Stability studies are an important task in developing finished veterinary medicinal products. The stability of medicines ensures that their therapeutic properties preserved for several years storage. should be under the close attention developer, given state control bodies do not check this indicator, and it is manufacturer's responsibility. In order to obtain data on change quality developed solution injection based celecoxib over time, influence various environmental factors, establish recommended storage conditions, tests resulting drug were conducted. As a result study, was proposed shelf life 2 28 days after first puncture stopper dark glass bottle at temperature 25 ± °C humidity 60 5 %, original packaging. Chromatograms demonstrate high similarity between standard sample if prominent peaks coincide retention time intensity. peak spectra confirm identity test sample. difference may indicate possible degradation or presence impurities. Further will next stage pre-registration testing aimed section “Data reflecting risk assessment environment”, which mandatory material part “Chemical, pharmaceutical biological documentation” dossier drug. Instructions created dispose expired defective batches drug, preventing harmful effects.

Language: Английский

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Identification of immune patterns in idiopathic pulmonary fibrosis patients driven by PLA2G7-positive macrophages using an integrated machine learning survival framework

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: Sept. 27, 2024

Language: Английский

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Ursolic Acid Restores Redox Homeostasis and Pro-inflammatory Cytokine Production in Denervation-Induced Skeletal Muscle Atrophy

Applied Biochemistry and Biotechnology, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 3, 2024

Language: Английский

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Initial Glutathione Depletion During Short-Term Bed Rest: Pinpointing Synthesis and Degradation Checkpoints in the γ-Glutamyl Cycle

Antioxidants, Journal Year: 2024, Volume and Issue: 13(12), P. 1430 - 1430

Published: Nov. 21, 2024

Hypokinesia triggers oxidative stress and accelerates the turnover of glutathione system via γ-glutamyl cycle. Our study aimed to identify regulatory checkpoints controlling intracellular levels. We measured intermediate substrates cycle in erythrocytes from 19 healthy young male volunteers before during a 10-day experimental bed rest. Additionally, we tracked changes levels specific metabolite ratios up 21 days Using gas chromatography-mass spectrometry internal standard technique, observed 9 ± 9% decrease first 5 rest, followed by an 11 increase 5th 10th day, nearly returning baseline ambulatory The cysteinyl-glycine-to-glutathione ratio, reflecting cyclotransferase activity (a key enzyme breakdown), rose 14 22% then fell 10 14% over subsequent days, again approaching cysteine-to-cysteine indicative cysteine synthetase (crucial for synthesis), increased 12 30% on day 29 41% results rest confirm those seen 10. By calculating ratio product concentration precursor concentration, assessed efficiency these enzymes turnover. These were corroborated directly measuring synthesis degradation rates vivo using stable isotope techniques. findings reveal significant kinetics initial potential therapeutic targets maintaining

Language: Английский

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[E

PubMed, Journal Year: 2024, Volume and Issue: 44(11), P. 2192 - 2200

Published: Nov. 20, 2024

To investigate the effect of E

Language: Английский

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