Combined BPA and DIBP Exposure Induced Intestinal Mucosal Barrier Impairment Through the Notch Pathway and Gut Microbiota Dysbiosis in Mice

Foods, Journal Year: 2025, Volume and Issue: 14(2), P. 214 - 214

Published: Jan. 12, 2025

Bisphenol A (BPA) and diisobutyl (DIBP) phthalate are widely used as typical plasticizers in food packaging. Plasticizers can be released from polymers, migrate into food, ingested by humans, leading to various health problems. However, little research has investigated the combined toxicity of BPA DIBP, particularly their intestinal toxicity. Our goal is analyse (50 mg/kg) DIBP (500 on intestines KM mice. Additionally, we tried find natural products that inhibit or prevent DIBP. The results indicated combination exposure resulted a reduction beneficial flora, an increase D-Lac levels (136 ± 14 μmol/L), permeability, activation notch pathway, decline stem cells (ISCs) goblet cells, compared single-exposure sources. Nevertheless, Rubus chingii Hu phenolic extract (RHPE) (200, 400 600 ameliorated DIBP-induced microbiota disruption mucosal barrier impairment inhibiting overactivation pathway. this study highlight potential risks human posed may help explain pathways enterotoxicity caused ingestion.

Language: Английский

The potential application of isoxanthohumol in inhibiting Clostridium perfringens infection by targeting the type IV pili

Microbial Cell Factories, Journal Year: 2025, Volume and Issue: 24(1)

Published: Feb. 21, 2025

Clostridium perfringens (C. perfringens) is an important zoonotic pathogen. The diseases such as necrotic enteritis (NE), enterotoxemia, gas gangrene and food poisoning caused by its infection seriously threaten the lives of both humans animals. However, under severe situation antibiotic resistance, development new antibacterial strategies or drugs deserves great attention. In this study, we selected virulence factor Type IV pili (TFP) C. target for drug screening. gliding motility, biofilm formation, cell adhesion activity natural compound isoxanthohumol (IXN) against were determined. Transmission electron microscopy (TEM), TFP gene transcription analysis Western blot used to detect expression PilA pilin. therapeutic effect IXN on was demonstrated through a mouse model. It confirmed that inhibits function down-regulating TFP-encoding genes two-component regulatory genes. conclusion, our study shows has potential inhibit in anti-infection applications.

Language: Английский

Preparation, characterization, and ex vivo evaluation of isoxanthohumol nanosuspension

International Journal of Pharmaceutics, Journal Year: 2024, Volume and Issue: 667, P. 124909 - 124909

Published: Nov. 8, 2024

Language: Английский