Glabridin Suppresses LPS-Induced Inflammatory Cascade by Targeting AMPK/JAK2/STAT3 Pathways in Macrophages DOI Creative Commons
Shaw‐Min Hou,

San Cheng,

Kao‐Chang Lin

et al.

Natural Product Communications, Journal Year: 2024, Volume and Issue: 19(12)

Published: Dec. 1, 2024

Objectives: Inflammation, a crucial biological response defending the body against harmful stimuli, has been central focus in medicinal research. Licorice root, rich bioactive compounds, contains glabridin (GBD), renowned natural flavonoid with anti-inflammatory and antioxidant properties. Despite its recognition, precise molecular mechanisms underlying GBD's effects remain subject of ongoing investigation. Methods: This study explores impact GBD on lipopolysaccharide (LPS)-induced inflammation RAW264.7 macrophages, aiming to unravel intricate pathways, specifically focusing potential involvement adenosine monophosphate-activated protein kinase (AMPK)/Janus 2 (JAK2)/signal transducer activator transcription 3 (STAT3) signaling pathways. The first determined effective dose by measuring production pro-inflammatory mediators LPS stimulation. novelty this lies subsequent examination under LPS-induced inflammation, an aspect that not thoroughly explored previous Results: inhibits cytokines nitric oxide (NO). It also suppresses expression iNOS IL-1β induced LPS. Furthermore, attenuates phosphorylation JAK2 STAT3, concurrently inhibiting AMPK phosphorylation. further elucidates relationship between JAK2/STAT3 using inhibitor, AG490, compound C (CC). Importantly, CC only but STAT3. observation strongly suggests modulates AMPK/JAK2/STAT3 pathways LPS-stimulated macrophages. Conclusion: reveals is promising agent novel inhibitory pathway, highlighting therapeutic value for inflammatory diseases.

Language: Английский

The Therapeutic Efficacy and Molecular Mechanisms of Artemisia argyi Essential Oil in Treating Feline Herpesvirus Infection via Nasal Drops DOI Creative Commons

Tian Wan,

Jinze Li, Jiayi Liu

et al.

Veterinary Sciences, Journal Year: 2025, Volume and Issue: 12(2), P. 80 - 80

Published: Jan. 23, 2025

This study aimed to explore the therapeutic potential and mechanisms of Artemisia argyi essential oil (AAEO) in managing feline herpesvirus type 1 (FeHV-1) infections. FeHV-1, causative agent viral rhinotracheitis (FVR), leads severe respiratory systemic complications cats. In this study, 35 cats were divided into blank, FeHV-1 infection, AAEO treatment groups (high, medium, low doses). vivo experiments demonstrated that alleviated clinical symptoms, reduced tissue damage, modulated immune responses. The AAEO-treated showed higher survival rates, stabilized body temperatures, less weight loss compared group. Histopathological analysis revealed improved integrity nasal, tracheal, bronchial tissues. Transcriptomic proteomic analyses identified critical pathways, such as IL-17 signaling, influenced by treatment, highlighting its role suppressing inflammation protecting integrity. vitro assays has concentration-dependent cytotoxicity kidney cells (F81) provides protective effects when used a pre-treatment. These findings suggest enhances host defenses mitigates FeHV-1-induced damage through modulation protection.

Language: Английский

Citations

0
Glabridin Suppresses Macrophage Activation by Lipoteichoic Acid In Vitro: The Crucial Role of MAPKs-IL-1β-iNOS Axis Signals in Peritoneal and Alveolar Macrophages DOI Creative Commons
Shaw‐Min Hou, Chunming Yang, Wei‐Chieh Huang

et al.

Biomolecules, Journal Year: 2025, Volume and Issue: 15(2), P. 174 - 174

Published: Jan. 24, 2025

Inflammation, a fundamental response to infection and injury, involves interactions among immune cells signaling molecules. Dysregulated inflammation contributes diseases such as autoimmune disorders cancer. Interleukin-1 beta (IL-1β), produced by macrophages in lipoteichoic acid (LTA) from Gram-positive bacteria, is key inflammatory mediator. Glabridin (GBD), bioactive compound licorice root, exhibits anti-inflammatory properties. This study investigates GBD’s effects on LTA-induced proinflammatory RAW 264.7 murine alveolar macrophages, MH-S, focusing IL-1β expression pathways. Cell viability assays confirmed that 20 μM GBD was non-cytotoxic. Confocal microscopy quantitative PCR showed significantly reduced fluorescence intensity, mRNA, protein levels. also inhibited inducible nitric oxide synthase (iNOS) (NO) production. Further analysis revealed suppressed NF-κB p65 nuclear translocation selectively modulated MAPK pathway activation reducing JNK p38 phosphorylation without affecting ERK. Studies using specific inhibitors demonstrated production reduction mechanistically linked inhibition. These findings highlight potential therapeutic agent for through its ability modulate critical mediators

Language: Английский

Citations

0
Crosstalk between ferroptosis and innate immune in diabetic kidney disease: mechanisms and therapeutic implications DOI Creative Commons
Jinyang Wang, Haonan Shi, Ye Seul Yang

et al.

Frontiers in Immunology, Journal Year: 2025, Volume and Issue: 16

Published: Feb. 28, 2025

Diabetic kidney disease (DKD) is a prevalent complication of diabetes mellitus (DM), and its incidence increasing alongside the number cases. Effective treatment long-term management DKD present significant challenges; thus, deeper understanding pathogenesis essential to address this issue. Chronic inflammation abnormal cell death in closely associate with development. Recently, there has been considerable attention focused on immune infiltration into renal tissues inflammatory response’s role progression. Concurrently, ferroptosis—a novel form death—has emerged as critical factor pathogenesis, leading increased glomerular filtration permeability, proteinuria, tubular injury, interstitial fibrosis, other pathological processes. The cardiorenal benefits SGLT2 inhibitors (SGLT2-i) patients have demonstrated through numerous large clinical trials. Moreover, further exploratory experiments indicate these drugs may ameliorate serum urinary markers inflammation, such TNF-α, inhibit ferroptosis models. Consequently, investigating interplay between innate responses for guiding future drug This review presents an overview within context DKD, beginning core mechanisms delving potential roles We will also analyze how aberrant cells, molecules, signaling pathways contribute Finally, we discuss interactions responses, well targeted therapeutic agents, based current evidence. By analyzing immunity aim provide insights development area.

Language: Английский

Citations

0
Ferulic acid protects against stress-induced myocardial injury in mice DOI

Siyong Li,

Peiyi He,

Jiahe Liu

et al.

Toxicology and Applied Pharmacology, Journal Year: 2025, Volume and Issue: 498, P. 117309 - 117309

Published: March 20, 2025

Language: Английский

Citations

0
Association of circulating cytokine levels and tissue-infiltrating myeloid cells with achalasia: results from Mendelian randomization and validation through clinical characteristics and single-cell RNA sequencing DOI
Xinyue Li,

An-Yi Xiang,

Xin‐Yang Liu

et al.

Journal of Gastroenterology, Journal Year: 2024, Volume and Issue: 59(12), P. 1079 - 1091

Published: Oct. 8, 2024

Language: Английский

Citations

1
Publication trends and research hotspots of IL-1: A bibliometric analysis of IL-1 with relevance to anesthesia in highly cited papers from WoSCC DOI Creative Commons
Zheng Li, Jie Ju, Xiaoqian Jia

et al.

Journal of Anesthesia and Translational Medicine, Journal Year: 2024, Volume and Issue: 3(3), P. 96 - 116

Published: Sept. 1, 2024

Language: Английский

Citations

0
Glabridin Suppresses LPS-Induced Inflammatory Cascade by Targeting AMPK/JAK2/STAT3 Pathways in Macrophages DOI Creative Commons
Shaw‐Min Hou,

San Cheng,

Kao‐Chang Lin

et al.

Natural Product Communications, Journal Year: 2024, Volume and Issue: 19(12)

Published: Dec. 1, 2024

Objectives: Inflammation, a crucial biological response defending the body against harmful stimuli, has been central focus in medicinal research. Licorice root, rich bioactive compounds, contains glabridin (GBD), renowned natural flavonoid with anti-inflammatory and antioxidant properties. Despite its recognition, precise molecular mechanisms underlying GBD's effects remain subject of ongoing investigation. Methods: This study explores impact GBD on lipopolysaccharide (LPS)-induced inflammation RAW264.7 macrophages, aiming to unravel intricate pathways, specifically focusing potential involvement adenosine monophosphate-activated protein kinase (AMPK)/Janus 2 (JAK2)/signal transducer activator transcription 3 (STAT3) signaling pathways. The first determined effective dose by measuring production pro-inflammatory mediators LPS stimulation. novelty this lies subsequent examination under LPS-induced inflammation, an aspect that not thoroughly explored previous Results: inhibits cytokines nitric oxide (NO). It also suppresses expression iNOS IL-1β induced LPS. Furthermore, attenuates phosphorylation JAK2 STAT3, concurrently inhibiting AMPK phosphorylation. further elucidates relationship between JAK2/STAT3 using inhibitor, AG490, compound C (CC). Importantly, CC only but STAT3. observation strongly suggests modulates AMPK/JAK2/STAT3 pathways LPS-stimulated macrophages. Conclusion: reveals is promising agent novel inhibitory pathway, highlighting therapeutic value for inflammatory diseases.

Language: Английский

Citations

0