Engineering of Microbial Cell Factories for Enhanced Acetic Acid and Ethanol Production Via Heterologous Overexpression of the CODH Gene in CO2 Fermentation DOI

Athmakuri Tharak,

G. Suresh,

Sreeram Kaveti

et al.

ACS Synthetic Biology, Journal Year: 2025, Volume and Issue: unknown

Published: April 15, 2025

This study evaluates the performance of carbon monoxide dehydrogenase (codh)-embedded strains in bench-scale microbial electrochemical systems (MES) for CO2 reduction to biofuels and biochemicals. fermentation efficiency was evaluated by comparing wild-type Clostridium acetobutylicum (Wild), a negative control E. coli strain lacking codh gene (NC-BL21), engineered (Eng) alone with IPTG induction (Eng+IPTG). Four were used, viz. Wild+E, NC-BL21+E, Eng+E, Eng+IPTG+E, poised potential -0.6 V applied working electrode. bicarbonate supplemented total inorganic (IC) concentration 40 g/L, retention time 60 h. The demonstrated enhanced metabolic compared strains, yielding maximum formic acid (2.1 g/L) acetic (9.3 under Eng+IPTG+E condition. Solventogenesis also influenced positively same system ethanol yield 3.9 substantially exceeding biochemicals observed (2.4 acid). exhibited superior cumulative yields (0.40 g/g), CODH-mediated charge flux stability (60 vs 5 wild type), upregulated expression key genes Wood-Ljungdahl pathway (WLP). Bioelectrochemical analysis elevated reductive catalytic currents, reduction, optimal transfer kinetics. highlights synergistic genetic engineering, specifically CODH overexpression, combined electro-fermentation biofuel biochemical production from C1 gases.

Language: Английский

Engineering of Microbial Cell Factories for Enhanced Acetic Acid and Ethanol Production Via Heterologous Overexpression of the CODH Gene in CO2 Fermentation DOI

Athmakuri Tharak,

G. Suresh,

Sreeram Kaveti

et al.

ACS Synthetic Biology, Journal Year: 2025, Volume and Issue: unknown

Published: April 15, 2025

This study evaluates the performance of carbon monoxide dehydrogenase (codh)-embedded strains in bench-scale microbial electrochemical systems (MES) for CO2 reduction to biofuels and biochemicals. fermentation efficiency was evaluated by comparing wild-type Clostridium acetobutylicum (Wild), a negative control E. coli strain lacking codh gene (NC-BL21), engineered (Eng) alone with IPTG induction (Eng+IPTG). Four were used, viz. Wild+E, NC-BL21+E, Eng+E, Eng+IPTG+E, poised potential -0.6 V applied working electrode. bicarbonate supplemented total inorganic (IC) concentration 40 g/L, retention time 60 h. The demonstrated enhanced metabolic compared strains, yielding maximum formic acid (2.1 g/L) acetic (9.3 under Eng+IPTG+E condition. Solventogenesis also influenced positively same system ethanol yield 3.9 substantially exceeding biochemicals observed (2.4 acid). exhibited superior cumulative yields (0.40 g/g), CODH-mediated charge flux stability (60 vs 5 wild type), upregulated expression key genes Wood-Ljungdahl pathway (WLP). Bioelectrochemical analysis elevated reductive catalytic currents, reduction, optimal transfer kinetics. highlights synergistic genetic engineering, specifically CODH overexpression, combined electro-fermentation biofuel biochemical production from C1 gases.

Language: Английский

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