FOXM1 affects oxidative stress, mitochondrial function, and the DNA damage response by regulating p21 in aging oocytes

Theriogenology, Journal Year: 2024, Volume and Issue: 229, P. 66 - 74

Published: Aug. 10, 2024

Language: Английский

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SCM-198 ameliorates the quality of postovulatory and maternally aged oocytes by reducing oxidative stress

Journal of Ovarian Research, Journal Year: 2024, Volume and Issue: 17(1)

Published: Aug. 31, 2024

Oocyte aging is a key constraint on oocyte quality, leading to fertilization failure and abnormal embryonic development. In addition, it likely generate unfavorable assisted reproductive technology (ART) outcomes. SCM-198, synthetic form of leonurine, was found rescue the rate fragmentation caused by postovulatory aging. Therefore, aim this study conduct more in-depth investigation SCM-198 exploring its relationship with aged oocytes after ovulation or maternal clarifying whether affects cell quality. The results indicate that, compared group, 50 µM group significantly improved sperm-egg binding increased oocytes, restoring spindle apparatus/chromosome structure, cortical granule distribution, ovastacin Juno protein distribution. showed normal mitochondrial low levels reactive oxygen species (ROS), small quantity early apoptosis group. Above all, in vivo supplementation effectively eliminated excess ROS reduced spindle/chromosome structural defects mouse oocytes. summary, these findings that inhibits excessive oxidative stress alters quality both vitro vivo.

Language: Английский

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The NAD+ precursor nicotinamide riboside protects against postovulatory aging in vitro

Journal of Assisted Reproduction and Genetics, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 26, 2024

Language: Английский

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Proteomic analysis implicates that postovulatory aging leads to aberrant gene expression, biosynthesis, RNA metabolism and cell cycle in mouse oocytes

Journal of Ovarian Research, Journal Year: 2022, Volume and Issue: 15(1)

Published: Oct. 14, 2022

In mammals, oocytes display compromised quality after experiencing a process of postovulatory aging. However, the mechanisms underlying are not yet fully understood. Here, we portrayed protein expression profile fresh and aging metaphase II (MII) mouse by means four-dimensional label-free quantification mass spectrometry (4D-LFQ).The analysis 4D-LFQ data illustrated that there were seventy-six differentially expressed proteins (DEPs) between two groups MII stage oocytes. Fifty-three DEPs up-regulated while twenty-three down-regulated in group, Gene Ontology (GO) revealed these mainly enriched regulation gene expression, biosynthesis, RNA metabolism cell cycle. Our detailed related to processes such as transcription, translation, post-translational modifications epigenome was changed; relative metabolic processes, alternative splicing, export from nucleus negative transcription polymerase promoter also altered.In conclusion, identified considerable discussed how they agreed with previous researches illustrating altered associated research provided new perspective on established theoretical support for practical methods control reverse

Language: Английский

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EFFECT OF EXTRACELLULAR VESICLES OF FOLLICULAR ORIGIN DURING in vitro MATURATION AND AGEING OF BOVINE OOCYTES ON EMBRYO DEVELOPMENT AFTER in vitro FERTILIZATION

Sel skokhozyaistvennaya Biologiya, Journal Year: 2023, Volume and Issue: 57(6), P. 1178 - 1187

Published: Jan. 1, 2023

ВЛИЯНИЕ ВНЕКЛЕТОЧНЫХ ВЕЗИКУЛ ФОЛЛИКУЛЯРНОГО ПРОИСХОЖДЕНИЯ В СРЕДЕ СОЗРЕВАНИЯ НА СПОСОБНОСТЬ ООЦИТОВ КОРОВ К ЭМБРИОНАЛЬНОМУ РАЗВИТИЮ in vitroПОСЛЕ СТАРЕНИЯ И ЭКСТРАКОРПОРАЛЬНОГО ОПЛОДОТВОРЕНИЯ  Е.Н.ШЕДОВА 1 , Г

Language: Русский

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