Spermatozoa centriole quality determined by FRAC may correlate with zygote nucleoli polarization—a pilot study
Journal of Assisted Reproduction and Genetics,
Journal Year:
2025,
Volume and Issue:
unknown
Published: Feb. 7, 2025
Spermatozoa
centriolar
defects
can
result
in
abnormal
zygote
functions.
Recently,
a
method
to
quantify
spermatozoa
was
developed
named
Fluorescence-Based
Ratiometric
Analysis
of
Sperm
Centrioles
(FRAC).
However,
whether
identified
by
FRAC
functions
not
tested.
Here,
we
quantified
using
FRAC,
and
centriole
function
assessed
imaging
Nucleolus
Precursor
Body
(NPB)
polarization
which
based
on
the
pattern
NPB
polarization.
Data
analyzed
at
couple
embryo
levels.
Subjects
were
divided
into
two
groups:
seven
couples
62
embryos
with
normal
centrioles
versus
eight
78
(140
from
15
total).
Patterned
statistically
significant
both
couple-
embryo-level
analyses
(p
<
0.0001
p
=
0.0024).
These
results
suggest
that
may
correlate
centrosome
via
scoring.
This
study
provides
foundation
for
more
extensive
studies
test
FRAC's
utility
assessing
quality.
Language: Английский
The centrosome – diverse functions in fertilization and development across species
Journal of Cell Science,
Journal Year:
2023,
Volume and Issue:
136(23)
Published: Dec. 1, 2023
ABSTRACT
The
centrosome
is
a
non-membrane-bound
organelle
that
conserved
across
most
animal
cells
and
serves
various
functions
throughout
the
cell
cycle.
In
dividing
cells,
known
as
spindle
pole
nucleates
robust
microtubule
to
separate
genetic
material
equally
into
two
daughter
cells.
non-dividing
mother
centriole,
substructure
of
centrosome,
matures
basal
body
cilia,
which
acts
signal-transducing
antenna.
centrosomes
their
substructures
are
important
for
embryonic
development
have
been
studied
extensively
using
in
vitro
mammalian
culture
or
vivo
invertebrate
models.
However,
there
considerable
differences
composition
during
different
aspects
vertebrate
development,
these
less
studied.
this
Review,
we
discuss
roles
played
by
centrosomes,
highlighting
divergent
features
species,
particularly
fertilization
development.
Language: Английский
CP110 and CEP135 Localize Near the Proximal Centriolar Remnants of Mice Spermatozoa.
PubMed,
Journal Year:
2024,
Volume and Issue:
2024
Published: Jan. 1, 2024
Centrioles
form
centrosomes
that
organize
microtubules,
assist
in
cell
structure,
and
nucleate
cilia
provide
motility
sensation.
Within
the
sperm,
centrosome
consists
of
two
centrioles
(proximal
distal
centriole)
a
pericentriolar
material
known
as
striated
column
capitulum.
The
centriole
nucleates
flagellum.
Mice
spermatozoa,
unlike
other
mammal
spermatozoa
(e.g.,
human
bovine),
have
no
ultra-structurally
recognizable
centrioles,
but
their
neck
has
centriolar
proteins
POC1B
FAM161A,
suggesting
mice
remnant
centrioles.
Here,
we
examine
whether
proteins,
CP110
CEP135,
found
bovine
are
also
mouse
neck.
is
tip
protein
controlling
ciliogenesis,
CEP135
centriole-specific
structural
base
canonical
most
types.
report
were
both
located
around
proximal
remnants
labeled
by
POC1B,
increasing
number
neck,
further
supporting
hypothesis
present
mice.
Language: Английский
Fly Fam161 is an essential centriole and cilium transition zone protein with unique and diverse cell type-specific localizations
Open Biology,
Journal Year:
2024,
Volume and Issue:
14(9)
Published: Sept. 1, 2024
Family
with
sequence
similarity
161
(Fam161)
is
an
ancient
family
of
microtubule-binding
proteins
located
at
the
centriole
and
cilium
transition
zone
(TZ)
lumen
that
exhibit
rapid
evolution
in
mice.
However,
their
adaptive
role
unclear.
Here,
we
used
flies
to
gain
insight
into
cell
type-specific
adaptations.
Fam161
sole
orthologue
FAM161A
FAM161B
found
flies.
Mutating
results
reduced
male
reproduction
abnormal
geotaxis
behaviour.
localizes
sensory
neuron
centrioles
specialized
TZ
(the
connecting
cilium)
a
manner,
sometimes
labelling
only
centrioles,
varying
lengths
are
longer
than
other
proteins,
defining
new
ciliary
compartment,
extra
distal
TZ.
These
findings
suggest
essential
protein
unique
localization
fruit
can
produce
Language: Английский
Comment on: ‘The proximal centriole age in spermatozoa is a potential reason for its different fate in the zygote after fertilization’ Uzbekov and Avidor-Reiss 2024
Open Biology,
Journal Year:
2024,
Volume and Issue:
14(3)
Published: March 1, 2024
Language: Английский
Renaissance in sperm cytoplasmic contribution to infertility
Journal of Assisted Reproduction and Genetics,
Journal Year:
2023,
Volume and Issue:
41(2), P. 293 - 296
Published: Nov. 13, 2023
Language: Английский
The cilium like region of the Drosophila bifurca spermatocyte: Elongation of a giant axoneme without intraflagellar transport
Cytoskeleton,
Journal Year:
2023,
Volume and Issue:
81(11), P. 529 - 538
Published: Dec. 10, 2023
Abstract
The
growth
of
the
ciliary
axonemes
mainly
depends
on
evolutionary
conserved
intraflagellar
transport
(IFT)
machinery.
However,
insect
spermatocytes
are
characterized
by
cilium‐like
regions
(CLRs)
that
elongate
in
absence
IFT.
It
is
generally
believed
dynamics
these
structures
relies
free
diffusion
soluble
tubulin
from
cytoplasm.
this
passive
process
could
allow
elongation
short
axonemes,
but
it
unclear
whether
simple
molecules
can
ensure
correct
assembly
elongated
structures.
To
decipher
point
we
analyzed
CLRs
held
primary
Drosophila
bifurca
.
These
consist
a
very
axoneme
grows
without
IFT
and,
therefore,
represent
good
model
which
to
evaluate
role
played
tubulin.
observation
wavy
microtubules
axonemal
lumen
fully
D.
may
be
consistent
with
within
lumen.
Progressive
consumption
used
for
at
apical
tip
result
gradient
sufficient
move
cytoplasm
end
forming
structure.
When
reaches
its
full
length,
not
drawn
and
accumulate
base
axoneme,
where
concentration
exceed
threshold
need
microtubule
polymerization.
presence
γ‐TuRCs
proximal
ends
supernumerary
enhance
their
nucleation.
Language: Английский
Формирование центриолей в раннем эмбриональном развитии млекопитающих. Новый взгляд в светепоследних открытий, "Природа"
Priroda,
Journal Year:
2023,
Volume and Issue:
10, P. 3 - 12
Published: Jan. 1, 2023
Центросома
—
небольшая
клеточная
органелла,
в
которой
сосредоточено
большое
количество
регуляторных
белков.
Будучи
также
центром
организации
микротрубочек,
центросома
представляет
собой
транспортный
узел
клетки,
из
которого
с
помощью
специфических
белков-моторов
по
микротрубочкам
осуществляется
как
центробежный,
так
и
центростремительный
транспорт
белковых
комплексов,
целых
органелл.
Хотя
за
последние
годы
достигнут
значительный
прогресс
идентификации
сотен
центросомальных
белков,
процесс
формирования
центросомы
еще
недостаточно
изучен.
В
настоящей
статье
изложены
результаты
исследования
процессе
раннего
эмбрионального
развития.
Language: Русский