Técnicas in vivo e in vitro sobre calidad y viabilidad de embriones en bovinos
Revista Científica Arbitrada Multidisciplinaria PENTACIENCIAS,
Journal Year:
2025,
Volume and Issue:
7(1), P. 274 - 282
Published: Jan. 17, 2025
En
las
últimas
décadas,
la
producción
de
embriones
in
vivo
e
vitro
han
revolucionado
ganadería,
lo
que
permitió
obtener
avances
mejoramiento
genético,
que,
en
efecto,
dichas
técnicas
se
llevaron
a
cabo
otras
especies
zootécnicas.
Ambas
son
fundamentales
para
evaluar
calidad
y
viabilidad
bovinos,
técnica
vivo,
da
cuatro
fases
el
tracto
reproductivo
vaca,
cuales
son;
superestimulación,
inseminación,
desarrollo,
recolección.
A
diferencia
recolectan
los
ovocitos
posterior
ello,
requiere
instalaciones
laboratorio
llevar
maduración,
fertilización
cultivo.
El
presente
artículo
tiene
sustentó
teórico
diversas
bases
datos
científicas,
así
como
gestión
selección
documentos
clave.
Se
espera
resultados
esta
investigación
proporcionen
información
sólida
sobre
diferencias
bovinos
manera,
permitan
conocer
ventajas
desventajas
biotecnologías
programas
reproducción
asistida.
Effects of Different Cryoprotectants on Cryopreservation of Sperm from Noble Scallop Mimachlamys nobilis
Journal of Marine Science and Engineering,
Journal Year:
2025,
Volume and Issue:
13(3), P. 607 - 607
Published: March 19, 2025
In
cryopreservation
technology,
the
choice
of
cryoprotectant
plays
a
crucial
role
in
cell
survival
and
function.
Different
types
cryoprotectants,
each
with
unique
protective
mechanisms,
mitigate
cellular
damage
from
ice
crystal
formation
during
freezing.
This
study
investigated
effects
different
concentrations
cryoprotectants
on
efficacy
noble
scallop
Mimachlamys
nobilis
sperm.
Six
were
tested,
including
four
permeable
(dimethyl
sulfoxide
(DMSO),
ethylene
glycerol
(EG),
propylene
(PG),
methanol
(MET))
two
non-permeable
(trehalose
(TRE),
fetal
bovine
serum
(FBS)).
The
results
showed
that
which
penetrate
membrane,
regulate
osmotic
pressure
inside
outside
cells
to
reduce
dehydration
damage.
Among
them,
10%
DMSO
provided
best
protection,
significantly
preserving
sperm
motility,
velocity,
morphology.
Non-permeable
although
unable
cells,
stabilized
extracellular
environment
at
higher
(such
as
FBS).
Additionally,
MET
FBS
exhibited
enhanced
increasing
concentration,
indicating
their
potential
reducing
structural
concentrations.
Morphological
observations
indicated
freezing
caused
varying
degrees
sperm,
flagellar
integrity
being
for
motility.
Overall,
selecting
an
appropriate
concentration
is
essential
efficient
M.
providing
valuable
reference
conserving
germplasm
resources
marine
species.
Language: Английский
Transparent Alumina Ceramics‐Based Microfluidic Chip Enables on‐Chip Cryopreservation for Mouse Oocyte
Siyu Xu,
No information about this author
Lei Yu,
No information about this author
Morgan K Wei
No information about this author
et al.
Advanced Functional Materials,
Journal Year:
2025,
Volume and Issue:
unknown
Published: March 20, 2025
Abstract
Oocyte
cryopreservation
plays
a
crucial
role
in
safeguarding
female
fertility
clinical
practice.
However,
commonly
used
methods
involving
tedious
and
time‐consuming
procedures,
as
well
uncontrolled
variability
between
operators,
which
can
easily
lead
to
cell
damage.
Here,
novel
method
for
cryopreserving
oocytes
using
transparent
alumina
ceramics‐based
microfluidic
chip
is
developed,
provides
an
integrated
platform
cryoprotectants
(CPAs)
loading/unloading,
freeze‐thaw
process
storage.
The
protocol
optimized
significantly
simplify
the
overall
process,
reduce
CPA
osmotic
damage,
achieve
high‐performance
oocyte
cryopreservation.
survival
rate
of
cryopreserved
with
this
reached
86.23%.
In
comparison
conventional
method,
recovered
exhibit
structural
stability,
functional
integrity,
genetic
normality
higher
developmental
capacity.
study
offers
low‐cost,
easy‐to‐operate,
high‐performance,
on‐chip
semi‐automatic
technique
oocytes,
great
significance
standardizing
automating
fertility,
has
important
potential
translational
applications.
Language: Английский
Evaluation of Cryopreservation of Bovine Ovarian Tissue by Analysis of Reactive Species of Oxygen, Toxicity, Morphometry, and Morphology
Veterinary Sciences,
Journal Year:
2024,
Volume and Issue:
11(11), P. 579 - 579
Published: Nov. 19, 2024
Ovarian
tissue
cryopreservation
has
been
widely
investigated
for
preserving
female
fertility.
In
the
present
study,
we
aimed
to
compare
effects
of
three
concentrations
(1,
1.5,
and
3
M)
dimethylsulfoxide
(DMSO)
on
vitrification
ovarian
tissue.
The
cortex
was
divided
into
control
vitrified
groups:
(i)
1
M-DMSO,
(ii)
1.5
(iii)
M-DMSO.
Follicles
from
all
fragments
were
analyzed
DMSO-induced
deleterious
effects,
morphological
morphometric
aspects,
concentration
reactive
oxygen
species.
Additionally,
cultured
assess
integrity
return
follicular
development
post-vitrification.
All
DMSO
resulted
in
a
higher
percentage
degenerated
preantral
follicles
than
before
process.
After
vitrification,
cryopreserved
showed
similar
percentages
intact
follicles;
however,
M
differed
control.
Analyzing
free
radical
production,
found
that
had
levels
oxidative
stress
lower
DMSO.
vitro
cultivation
vitrified/warmed
fragments,
exhibited
morphologically
other
concentrations.
Therefore,
suggest
bovine
can
be
situ
with
greater
efficiency
Language: Английский