Synthesis of trypsin protected CsPbCl3 fluorescent nanocrystals for hydroxyl radical sensing DOI Creative Commons
Suresh Kumar Kailasa,

Kartik Pankajbhai Makwana,

Madhura Pradeep Deshpande

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 17, 2024

Abstract Water-dispersible perovskite nanocrystals (PNCs) show promising applications in recognizing ionic and molecular species because of their excellent optical properties. However, lead halide PNCs have some limitations when they are used as probes for sensing aqueous media. Here, we introduce trypsin (Try) a bioligand the synthesis cesium chloride (CsPbCl3) with high water stability. The as-fabricated Try-CsPbCl3 λEm/Ex at 433/370 nm quantum yield 17.26%. fluorescence emission spectral characteristics demonstrated that water-stable acted fluorescent probe detection hydroxyl radical (•OH) via turn-off mechanism. PNCs-based approach displayed good selectivity water, showing wider linear range (0.01–5 µM) remarkable limit 3.10 nM radical. as-prepared were to be facile •OH samples, which signifies exhibited broad cell imaging.

Language: Английский

Synthesis of trypsin-protected CsPbCl3 fluorescent nanocrystals for hydroxyl radical sensing DOI Creative Commons
Suresh Kumar Kailasa,

Kartik Pankajbhai Makwana,

Madhura Pradeep Deshpande

et al.

Microchimica Acta, Journal Year: 2025, Volume and Issue: 192(4)

Published: March 10, 2025

Water-dispersible perovskite nanocrystals (PNCs) show promising applications in recognizing ionic and molecular species because of their excellent optical properties. However, lead halide PNCs have some limitations when they are used as probes for sensing aqueous media. Here, we introduce trypsin (Try) a bioligand the synthesis cesium chloride (CsPbCl3) with high water stability. The as-fabricated Try-CsPbCl3 λEm/Ex at 433/370 nm quantum yield 17.26%. fluorescence emission spectral characteristics demonstrated that water-stable acted fluorescent probe detection hydroxyl radical (•OH) via turn-off mechanism. PNCs-based approach displayed good selectivity water, showing wider linear range (0.01–5 µM) remarkable limit 3.10 nM radical. were to be facile •OH samples, which signifies exhibited broad real samples.

Language: Английский

Citations

0
A Ratiometric Fluorescence Probe Based on Silver Nanoclusters and CdSe/ZnS Quantum dots for the Detection of Hydrogen Peroxide by Aggregation and Etching DOI
Yuanyuan Zhang, Xin Zhang,

Hedan Xu

et al.

Journal of Fluorescence, Journal Year: 2024, Volume and Issue: unknown

Published: June 22, 2024

Language: Английский

Citations

2
Progress and limitations in reactive oxygen species quantitation DOI

Eleni Spanolios,

Riley E. Lewis,

R. Caldwell

et al.

Chemical Communications, Journal Year: 2024, Volume and Issue: 60(86), P. 12487 - 12501

Published: Jan. 1, 2024

Reactive oxygen species (ROS) can be quantified using fluorescence, electrochemical, and electron paramagnetic resonance spectroscopy techniques. Detection of ROS is critical in a wide range chemical biological systems.

Language: Английский

Citations

2
Near-infrared modulated photothermal/nanozymatic dual-modal strategy for combating cancer via molybdenum selenide nanourchins DOI Creative Commons
Yifan Li, Chao Huang, Zhengbin Wang

et al.

Cancer Nanotechnology, Journal Year: 2024, Volume and Issue: 15(1)

Published: Nov. 4, 2024

Breast cancer, a major health concern globally, often faces challenges in the non-invasive elimination of cancer cells. This has prompted researchers to explore alternative strategies, such as photothermal therapy (PTT), which takes advantage location breast lesions. Additionally, tumor microenvironment with high hydrogen peroxide (H2O2) provides key prerequisite for treating tumors nanozymes, especially those peroxidase (POD)-like activity. However, overexpression TrxR1, maintains redox homeostasis cells, limits reactive oxygen species (ROS)-based cytotoxic therapy. study aims present molybdenum selenide nano urchins (MoSe2 NUs) excellent conversion efficiency, POD-like activity and selective inhibition TrxR1 achieve enhanced nanocatalytic combating cancer. The characterization MoSe2 NUs synthesized by hydrothermal method was identified TEM, XRD, Zeta potential DLS. Whether conjunction NIR possessed activities or not via TMB colorimetric method. characteristics excited near-infrared were recorded an infrared thermal imaging camera. antitumor effect detected cell death staining, apoptosis assay animal experiments. apoptosis-related protein expression Western blot Immunohistochemistry. activity, TrxR1. Furthermore, can enhance their allowing accurate treatment under (NIR) light. plus alone exhibited vivo rates 69.7% 35.1%, respectively. Mechanistically, NIR-regulated induced potent cells downregulating elevating intracellular ROS, thereby leading caspase-3 cleavage. demonstrated that irradiation precisely efficiently treat have great clinical application.

Language: Английский

Citations

0
Synthesis of trypsin protected CsPbCl3 fluorescent nanocrystals for hydroxyl radical sensing DOI Creative Commons
Suresh Kumar Kailasa,

Kartik Pankajbhai Makwana,

Madhura Pradeep Deshpande

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 17, 2024

Abstract Water-dispersible perovskite nanocrystals (PNCs) show promising applications in recognizing ionic and molecular species because of their excellent optical properties. However, lead halide PNCs have some limitations when they are used as probes for sensing aqueous media. Here, we introduce trypsin (Try) a bioligand the synthesis cesium chloride (CsPbCl3) with high water stability. The as-fabricated Try-CsPbCl3 λEm/Ex at 433/370 nm quantum yield 17.26%. fluorescence emission spectral characteristics demonstrated that water-stable acted fluorescent probe detection hydroxyl radical (•OH) via turn-off mechanism. PNCs-based approach displayed good selectivity water, showing wider linear range (0.01–5 µM) remarkable limit 3.10 nM radical. as-prepared were to be facile •OH samples, which signifies exhibited broad cell imaging.

Language: Английский

Citations

0