Breast organoid suspension cultures maintain long-term estrogen receptor expression and responsiveness DOI Creative Commons
Joan S. Brugge, Kung-Chi Chang, F Silvestri

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: June 17, 2024

Abstract Organoid cultures offer a powerful technology to investigate many different aspects of development, physiology, and pathology diverse tissues. Unlike standard tissue culture primary breast epithelial cells, organoids preserve the lineages architecture normal tissue. However, existing organoid methods are tedious, difficult scale, do not robustly retain estrogen receptor (ER) expression responsiveness in long-term culture. Here, we describe modified method generate maintain as suspension reconstituted basement membrane (™Matrigel). This improves growth uniformity compared conventional Matrigel dome embedding method, while maintaining fidelity three major lineages. Using this adopted able passage purified hormone sensing (HS) cells that ER upon stimulation system presents valuable platform study events involved initiation evolution ER-positive cancer.

Language: Английский

Next-Generation Breast Organoids Capture Human Organogenesis With High-Resolution Live Imaging DOI Creative Commons
Gat Rauner, Nicole Traugh,

Colin J. Trepicchio

et al.

Published: Feb. 5, 2025

Organoids have emerged as a powerful tool for modeling tissue growth and diseases. In this study, we introduce groundbreaking organotypic culture technique that replicates the morphology, scale, heterogeneity of human breast tissue, includes mesenchymal-like stromal component. A standout feature approach is use long-term live imaging at high temporal resolution to directly observe stem cell dynamics during organogenesis, from single cells mature organ tissue. The system adaptable throughput applications allows genetic manipulation cells. Real-time ex-vivo formation reveals non-canonical process ductal-lobular morphogenesis branching, de-novo generation supportive stroma. Incorporating patient-derived multiple donors offers an enhanced representation spectrum individual responses impacts distinct exposures. While developed principles technology can serve model development similar systems in other tissues, where organoids do not merely reproduce but their regeneration also be observed studied. addition, provides quantitative experimental study mechanisms embryogenesis, development, organization biomechanics plays important role.

Language: Английский

Citations

0
Methods and Models in Mammary Gland Biology and Breast Cancer Research DOI Creative Commons
Johanna I. Englund, Zuzana Koledová

Journal of Mammary Gland Biology and Neoplasia, Journal Year: 2025, Volume and Issue: 30(1)

Published: March 8, 2025

The collection on Methods and Models in Mammary Gland Biology Breast Cancer Research showcases recent advances tools models that enhance our understanding of mammary gland development breast cancer. This includes sixteen articles, collectively addressing approaches to investigate key aspects biology tumorigenesis, including hormonal signaling, tissue architecture, tumor microenvironment, species-specific development. issue highlights innovations such as optimized progesterone receptor reporters, improved menopause models, 3D-printed epithelial structures. It also features advancements organoid-based studies, situ labeling proliferation large animals, preclinical for cancer prevention, high-resolution imaging techniques. Methodologies studying macrophage-cancer cell interactions lysosomal function are provided step-by-step protocols. Additionally, review articles provide insights into diverse mammalian organoid systems, rat strategies modeling metastasis. Together, these contributions advance research by refining experimental approaches, expanding model diversity, fostering translational applications

Language: Английский

Citations

0
Breast organoid suspension cultures maintain long-term estrogen receptor expression and responsiveness DOI Creative Commons
Kung-Chi Chang, F Silvestri,

Michael Oliphant

et al.

npj Breast Cancer, Journal Year: 2024, Volume and Issue: 10(1)

Published: Dec. 19, 2024

Abstract Organoid cultures offer a powerful technology to investigate many different aspects of development, physiology, and pathology diverse tissues. Unlike standard tissue culture primary breast epithelial cells, organoids preserve the lineages architecture normal tissue. However, existing organoid methods are tedious, difficult scale, do not robustly retain estrogen receptor (ER) expression responsiveness in long-term culture. Here, we describe modified method generate maintain as suspension reconstituted basement membrane (™Matrigel). This improves growth uniformity compared conventional Matrigel dome embedding method, while maintaining fidelity three major lineages. Using this adopted able passage purified hormone sensing (HS) cells that ER upon stimulation system presents valuable platform study events involved initiation evolution ER-positive cancer.

Language: Английский

Citations

1
Breast organoid suspension cultures maintain long-term estrogen receptor expression and responsiveness DOI Creative Commons
Joan S. Brugge, Kung-Chi Chang, F Silvestri

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: June 17, 2024

Abstract Organoid cultures offer a powerful technology to investigate many different aspects of development, physiology, and pathology diverse tissues. Unlike standard tissue culture primary breast epithelial cells, organoids preserve the lineages architecture normal tissue. However, existing organoid methods are tedious, difficult scale, do not robustly retain estrogen receptor (ER) expression responsiveness in long-term culture. Here, we describe modified method generate maintain as suspension reconstituted basement membrane (™Matrigel). This improves growth uniformity compared conventional Matrigel dome embedding method, while maintaining fidelity three major lineages. Using this adopted able passage purified hormone sensing (HS) cells that ER upon stimulation system presents valuable platform study events involved initiation evolution ER-positive cancer.

Language: Английский

Citations

0