Surfactin production from Bacillus cereus AHMNAZ1 and its potential applications DOI

Alaa Atef,

Mostafa M. Abo Elsoud, Heba I. Elkhouly

et al.

Journal of Cellular Biotechnology, Journal Year: 2023, Volume and Issue: 10(1), P. 45 - 74

Published: Dec. 1, 2023

BACKGROUND: Biosurfactants are surfactants derived from several types of microorganisms such as bacteria, yeasts and fungi membrane components or secondary metabolites. OBJECTIVE: To increase BS productivity a biocompatible, low-toxic substitute for chemical employed in modern industry the huge variety applications they used in. METHODS: Different media were isolation biosurfactant producers. The most potent bacterial isolate was analysed by 16S-rRNA. Plackett Burman Box-Behnken Designs optimization conditions. purified characterized. RESULTS: Only one strain demonstrated great productivity, excellent emulsifying capability (54.5±0.1%) oil spreading activity which Bacillus cereus AHMNAZ1 with accession number OP714421.1. lipopeptide nature, identified surfactin stable has Critical Micelle Concentration 60 mg/L. Isolated showed against Colon Carcinoma Breast cell lines can be antimicrobial agent. CONCLUSION: study found cheaper way creating biosurfactants agro-industrial wastes, delivering twofold benefit lowering environmental pollution manufacturing useful biotechnological products (biosurfactants) high thermostability. Moreover, it bioremediation oil-polluted soils medical fields.

Language: Английский

Isolation and characterization of two glycolipopeptids biosurfactants produced by a Lactiplantibacillus plantarum OL5 strain isolated from green olive curing water DOI

Ameni Ben Ammar,

Mouna Bouassida,

Amir Bouallegue

et al.

World Journal of Microbiology and Biotechnology, Journal Year: 2023, Volume and Issue: 39(11)

Published: Sept. 16, 2023

Language: Английский

Citations

4
Decolorization of methyl red using Bacillus thuringiensis RI16 strain: Enhanced bacterial treatment by SPB1 biosurfactant addition DOI Creative Commons
Inès Mnif,

Raouia Fendri,

Dhouha Ghribi

et al.

Water Practice & Technology, Journal Year: 2022, Volume and Issue: 17(12), P. 2570 - 2580

Published: Nov. 14, 2022

Abstract In this work, we evaluate the ability of a novel isolated bacteria identified as Bacillus thuringiensis RI16 (KM111604) to decolorize methyl red. Results showed its efficiency for treatment azo-dye-contaminated wastewaters under static conditions at pH values ranging from 7 9. The could tolerate higher doses dyes it was able up 1000 mg/L. For better red decolorization, potential use subtilis SPB1-derived lipopeptide biosurfactant. study accelerate decolorization rate and slightly maximized an optimal concentration 0.025%. Compared with synthetic surfactants, bacterial surfactant more efficient observe drastic decrease inhibition by cetyl trimethylammonium bromide (CTAB) sodium dodecyl sulfate (SDS) additions. Also, studies ensured that removal strain be due extracellular enzymatic activities. SPB1 biosurfactant improve enzyme activities and/or synthesis diffusion.

Language: Английский

Citations

7
Identification of Different Lipopeptides Isoforms Produced by Bacillus mojavensis BI2 and Evaluation of Their Surface Activities for Potential Environmental Application DOI
Inès Mnif, Roser Segovia, Amir Bouallegue

et al.

Journal of Polymers and the Environment, Journal Year: 2023, Volume and Issue: 31(6), P. 2668 - 2685

Published: Jan. 19, 2023

Language: Английский

Citations

3
Bioremediation of heavy metal-contaminated environment: developed strategies and potential use of biosurfactants as chelators DOI Creative Commons
Inès Mnif,

Salwa Mekki,

Dhouha Ghribi

et al.

Water Practice & Technology, Journal Year: 2023, Volume and Issue: 18(10), P. 2355 - 2371

Published: Sept. 12, 2023

Abstract Heavy metal pollution damages the ecosystems and presents a major problem for public health. Thus, an urgent need was developed to decrease high levels of heavy metals in soil aquatic environments. With this aim, numerous physicochemical strategies were developed. However, they are money-consuming, require use energy chemical additives can release secondary compounds that pollute cause great damage environment. Then, biological methods based on investigation bacteria, fungi plants along with their derived active metabolites became best alternatives. Using plant capacities, different phytoremediation such as phytoextraction, phytovolatilization, rhizofiltration phytostabilization. Regarding bioremediation, bacterial biosorption metals, biolixiviation lagooning offer potential environmental cleaning. Additionally, metabolites, biosurfactants, is well-studied. Generally, class structurally very varied molecules commonly synthesized by many microorganisms amphiphilic character. Owing anionic charge, have capacity sequestrate permitting elimination. Glycolipids lipopeptides among most recognized biosurfactants interesting chelating properties.

Language: Английский

Citations

3
Treatment of heavy metals contaminated water: use of B. mojavensis BI2 derived lipopeptide and palm waste flour DOI Creative Commons
Inès Mnif,

Salwa Mekki,

Dhouha Ghribi

et al.

Water Science & Technology, Journal Year: 2022, Volume and Issue: 86(5), P. 1083 - 1094

Published: Aug. 10, 2022

Abstract In the present work, we demonstrated potential use of newly identified lipopeptides produced by B. mojavensis BI2 along with palm waste flour for bioremediation heavy metals contaminated water. The enhancement radish seeds germination was used to evaluate treatment efficiency. Firstly, better in order 3.8, 2.52, 1.5 and 5 were recorded respectively 200 mg/L copper, lead, cobalt mercury respective lipopeptide quantities 200, 300, 400 mg/L. When studying sequestration increasing concentration, effective. Secondly, a mixed bioprocess evaluated using as sequester improver. Optimal biosorption obtained 10 g/l waste, 1,000 mg/l metal 1 hour. addition improves efficiency significantly.

Language: Английский

Citations

5
A new bacterial-derived biosurfactant for biotechnological applications in the oil industry: production, optimization, biosurfactant functional and physicochemical characterization DOI Creative Commons

Marwa Elghoul,

Mouna Bouassida,

Dhouha Ghribi

et al.

Water Practice & Technology, Journal Year: 2023, Volume and Issue: 18(4), P. 810 - 830

Published: March 20, 2023

Abstract Aiming the potential application of lipopeptide biosurfactant (BioS) in bioremediation, we studied its production by a novel, isolated strain Bacillus sp. MI27. Using experimental design methodology, novel medium composed 2% sucrose, 0.27% Na2HPO4, 0.2% ammonium sulfate, 0.02% NaCl, CaCl2, MgSO4, 0.001% MnSO4, 0.06% KH2PO4, 0.005% FeSO4 and ZnSO4 was optimized. With this composition, MI27 produces 1.4 g/L BioS with maximum surface tension (ST) reduction 23 mN/m dispersion diameter around 10 cm. Emulsifying foaming activities have been also confirmed. The critical micelle concentration (CMC) value about 120 mg/L corresponding ST mN/m. emulsification index (EI) were 12 cm 45% at 1,000 respectively. Moreover, capacity, stable over 1 h incubation, 80% mg/L. Additionally, effect different pH, temperature salinities on activity stability. Obtained results showed interesting extreme physicochemical conditions, especially acidic alkaline pH values, high low temperatures higher salinities. All these characteristics enable possible water treatment biotechnology under diverse environmental conditions.

Language: Английский

Citations

2
Formulation of a Culture Medium to Optimize the Production of Lipopeptide Biosurfactant by a New Isolate of Bacillus sp.: A Soil Heavy Metal Mitigation Approach DOI Creative Commons

Sahar Kalvandi,

Hamidreza Garousin,

Ahmad Ali Pourbabaee

et al.

Frontiers in Microbiology, Journal Year: 2022, Volume and Issue: 13

Published: March 8, 2022

This research aimed to optimize a lipopeptide biosurfactant produced from Bacillus sp. SHA302 due its high efficiency of heavy metal release in soil. The results demonstrated that the capacity alone increased with increasing concentration. Among treatments different concentrations plus acid, highest rates 53.8% ± 1.4 and 39.3% 1.7 for Zn Pb, respectively, were observed critical micelle concentration (CMC) + HCl treatment. factorial experiment designed optimizing production showed among five inexpensive carbon sources six mineral nitrogen sources, sugar beet molasses (1%) ammonium chloride (0.1%) most efficient lowering surface tension (ST) culture media 32.2 0.76 mN/m. second step was Plackett-Burman design 11 factors four pH, chloride, magnesium sulfate, significantly affected (P < 0.05) changes ST production. third done using response methodology (RSM) central composite design. pH 7.3, 1.5 g/l 0.3 10% yielded values 29.2 0.71 mN/m 5.74 0.52 two variables production, which reached their optimal levels.

Language: Английский

Citations

3
Identification of surfactin lipopeptides isoforms produced by a newly isolated strain Bacillus sp. MI27 with potential use as main ingredients in detergent formulation: Application of mixture design for the formulation of a bio-based detergent DOI Creative Commons
Inès Mnif, Melanie Ghoul,

Mouna Bouassida

et al.

Water Practice & Technology, Journal Year: 2024, Volume and Issue: 19(3), P. 629 - 645

Published: Feb. 24, 2024

Abstract The purification and characterization of lipopeptide biosurfactants produced by Bacillus sp. MI27 permits to identify two clusters cyclic linear surfactin isoforms. Aiming for a potential application in industrial biotechnology, we studied its washing capacity. results show good ability wash oil stains, motor mayonnaise evaluated the determination % stain removal tissue bleaching indices after washing. Optimal capacities were unregistered 300 400 mg/L at pH values ranging from 5 9 temperatures 20 40°C. Using 4-factor mixing design, optimized liquid formula composed only BioS; silicate; carboxymethyl cellulose calcium phosphate. efficiency is order 43.46% petroleum 49.10% with percentage reduction surface tension about 55.79% corresponding 25.7 mN/m.

Language: Английский

Citations

0
Basis for the formulation of Bacillus subtilis SPB1 biosurfactant to achieve low interfacial tension and high emulsification activities: development of a bio-based formula with potential use for personal care products DOI Creative Commons

Mouna Bouassida,

Nada Fourati,

Inès Mnif

et al.

Water Practice & Technology, Journal Year: 2023, Volume and Issue: 18(5), P. 1125 - 1137

Published: April 10, 2023

Abstract Thanks to their multifunctional properties, such as foaming, emulsifying and skin hydrating activities biosurfactants are potentially used in the cosmetic industry. In present work, firstly, cream formulations using chemical surfactant agents lipopeptide isolated from Bacillus subtilis SPB1 were investigated. The efficiency of selected formula, containing 15% biosurfactant, was tested its physicochemical properties satisfying. fact, it shows a spreading ability 23 mm, water activity 0.72 pH value 5. addition, biological interesting showing zone inhibition against Gram-positive bacteria ranging 8 15 mm. Moreover, presents an antiradical effect diphenyl-1-picrylhydrazyl (DPPH) with IC50 order 68 mg/mL. Second, seeking highly stable biosurfactant formulation for easy commercial trend, combination Gum Arabic (GA) glycerol optimized Box–Behnken (BB) design. As analysed, model predicts optimal surface tension (ST) equal 40.18 ± 0.39 mN/m when respective values percentages BioS, GA 72.62, 7.5 1.5%. Under these conditions, emulsification index (EI)-24% is around 81.76 0.14%.

Language: Английский

Citations

1
Role of Biosurfactants in Biocidal Activity and Wound Healing DOI

John Adewole Alara,

Oluwaseun Ruth Alara,

Nour Hamid Abdurahman

et al.

Springer eBooks, Journal Year: 2023, Volume and Issue: unknown, P. 399 - 437

Published: Jan. 1, 2023

Language: Английский

Citations

0