High affinity of host human microRNAs to SARS-CoV-2 genome: An in silico analysis DOI Creative Commons
Saeideh Jafarinejad‐Farsangi,

Maryam Moazzam Jazi,

Farzaneh Rostamzadeh

et al.

Non-coding RNA Research, Journal Year: 2020, Volume and Issue: 5(4), P. 222 - 231

Published: Nov. 21, 2020

Coronavirus disease 2019 (COVID-19) caused by a novel betacoronavirus named severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has attracted top health concerns worldwide within few months after its appearance. Since viruses are highly dependent on the host small RNAs (microRNAs) for their replication and propagation, in this study, miRNAs targeting SARS-CoV-2 genome differentially expressed genes (DEGs) lungs of patients infected with SARS-CoV-2, were predicted.

Language: Английский

The 2020 race towards SARS-CoV-2 specific vaccines DOI Creative Commons
Tomasz M. Karpiński, Marcin Ożarowski, Agnieszka Seremak‐Mrozikiewicz

et al.

Theranostics, Journal Year: 2020, Volume and Issue: 11(4), P. 1690 - 1702

Published: Dec. 16, 2020

The global outbreak of a novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) highlighted requirement for two pronged clinical interventions such as development effective vaccines and therapeutic options medium-to-severe stages "coronavirus disease 2019" (COVID-19). Effective vaccines, if successfully developed, have been emphasized to become the most strategy in fight against COVID-19 pandemic. Basic research advances biotechnology genetic engineering already provided excellent progress groundbreaking new discoveries field biology its epidemiology. In particular, vaccine characterization capsid structure identification antigens that can targets vaccines. experimental requires plethora molecular techniques well strict compliance with safety procedures. data integrity, cross-validation results, appropriated studies from perspective efficacy potently side effects recently hotly discussed topic. this review, we present an update on latest ongoing race develop 52 different SARS-CoV-2. Our analysis is focused registered trials (current November 04, 2020) fulfill international criteria development. requirements benefits risks diverse types SARS-CoV-2 are including those containing whole-virus live-attenuated subunit mRNA DNA live vector also plant-based formulation coronavirus-like particle (VLP). challenges associated distribution, long-term effectiveness discussed.

Language: Английский

Citations

91

The landscape of antibody binding in SARS-CoV-2 infection DOI Creative Commons
Anna S. Heffron, Sean J. McIlwain, Maya F. Amjadi

et al.

PLoS Biology, Journal Year: 2021, Volume and Issue: 19(6), P. e3001265 - e3001265

Published: June 18, 2021

The search for potential antibody-based diagnostics, vaccines, and therapeutics pandemic severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has focused almost exclusively on the spike (S) nucleocapsid (N) proteins. Coronavirus membrane (M), ORF3a, ORF8 proteins are humoral immunogens in other coronaviruses (CoVs) but remain largely uninvestigated SARS-CoV-2. Here, we use ultradense peptide microarray mapping to show that SARS-CoV-2 infection induces robust antibody responses epitopes throughout proteome, particularly M, which 1 epitope achieved excellent diagnostic accuracy. We map 79 B cell proteome demonstrate antibodies develop response bind homologous sequences 6 known human CoVs. also confirm reactivity against 4 of our top-ranking by enzyme-linked immunosorbent assay (ELISA). Illness severity correlated with increased 9 S, N, ORF3a population. Our results previously unknown, highly reactive full CoV

Language: Английский

Citations

81

Host mitochondrial transcriptome response to SARS-CoV-2 in multiple cell models and clinical samples DOI Creative Commons
Brendan Miller, Ana Silverstein,

Melanie Flores

et al.

Scientific Reports, Journal Year: 2021, Volume and Issue: 11(1)

Published: Jan. 8, 2021

Abstract SARS-CoV-2 induces a muted innate immune response compared to other respiratory viruses. Mitochondrial dynamics might partially mediate this effect of on immunity. Polypeptides encoded by open reading frames SARS-CoV and have been shown localize mitochondria disrupt Antiviral Signaling (MAVS) protein signaling. Therefore, we hypothesized that would distinctly regulate the mitochondrial transcriptome. We analyzed multiple publicly available RNASeq data derived from primary cells, cell lines, clinical samples (i.e., BALF lung). report did not dramatically (1) mtDNA-encoded gene expression or (2) MAVS expression, (3) downregulated nuclear-encoded (NEM) genes related cellular respiration Complex I.

Language: Английский

Citations

79

Structure of the SARS-CoV-2 Nsp1/5′-Untranslated Region Complex and Implications for Potential Therapeutic Targets, a Vaccine, and Virulence DOI Creative Commons
Naveen Vankadari,

Nandhini Nisha Jeyasankar,

Wilma Jerom Lopes

et al.

The Journal of Physical Chemistry Letters, Journal Year: 2020, Volume and Issue: 11(22), P. 9659 - 9668

Published: Nov. 2, 2020

SARS-CoV-2 is the cause of ongoing Coronavirus disease 19 (COVID-19) pandemic around world causing pneumonia and lower respiratory tract infections. In understanding pathogenicity mechanism action, it essential to depict full repertoire expressed viral proteins. The recent biological studies have highlighted leader protein Nsp1 importance in shutting down host production. Besides, still enigmatic how regulates for translation. Here we report novel structure from complex with SL1 region 5'UTR SARS-CoV-2, its factual interaction corroborated enzyme kinetics experimental binding affinity studies. also address recognizes self RNA toward translational regulation by further recruitment 40S ribosome. With aid molecular dynamics simulations, demonstrated real-time stability functional Nsp1/SL1 complex. potential inhibitors their mode action block protein/RNA formation. This enhance our first synthesized human cell regulate translation host. Understanding interplay ribosome will open arena exploring development live attenuated vaccines effective therapeutic targets this disease.

Language: Английский

Citations

77

High affinity of host human microRNAs to SARS-CoV-2 genome: An in silico analysis DOI Creative Commons
Saeideh Jafarinejad‐Farsangi,

Maryam Moazzam Jazi,

Farzaneh Rostamzadeh

et al.

Non-coding RNA Research, Journal Year: 2020, Volume and Issue: 5(4), P. 222 - 231

Published: Nov. 21, 2020

Coronavirus disease 2019 (COVID-19) caused by a novel betacoronavirus named severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has attracted top health concerns worldwide within few months after its appearance. Since viruses are highly dependent on the host small RNAs (microRNAs) for their replication and propagation, in this study, miRNAs targeting SARS-CoV-2 genome differentially expressed genes (DEGs) lungs of patients infected with SARS-CoV-2, were predicted.

Language: Английский

Citations

76