Thermal Characterization and Interaction of the Subunits from the Multimeric Bacteriophage Endolysin PlyC DOI Creative Commons

J. Todd Hoopes,

Ryan D. Heselpoth,

Frederick P. Schwarz

et al.

Biology, Journal Year: 2023, Volume and Issue: 12(10), P. 1277 - 1277

Published: Sept. 25, 2023

Bacteriophage endolysins degrade the bacterial peptidoglycan and are considered enzymatic alternatives to small-molecule antibiotics. In particular, multimeric streptococcal endolysin PlyC has appealing antibacterial properties. However, a comprehensive thermal analysis of is lacking, which necessary for evaluating its long-term stability downstream therapeutic potential. Biochemical kinetic-based methods were used in combination with differential scanning calorimetry investigate structural, kinetic, thermodynamic various subunits domains. The holoenzyme structure irreversibly compromised due partial unfolding aggregation at 46 °C. Unfolding catalytic subunit, PlyCA, instigates this event, resulting kinetic inactivation endolysin. contrast PlyCB octamer (the cell wall-binding domain) thermostable, denaturing ~75 isolation PlyCA or alone altered their Contrary holoenzyme, unfolds uncooperatively thermodynamically destabilized, whereas reversibly dissociates into monomers forms an intermediate state 74 °C phosphate-buffered saline each subunit subsequently 92 Adding folded PlyCB, followed by cooling, allowed vitro reconstitution active holoenzyme.

Language: Английский

The antibacterial activity of a novel highly thermostable endolysin, LysKP213, against Gram-negative pathogens is enhanced when combined with outer membrane permeabilizing agents DOI Creative Commons

Dingjian Chu,

Jing Lan,

Lu Liang

et al.

Frontiers in Microbiology, Journal Year: 2024, Volume and Issue: 15

Published: Oct. 8, 2024

Phages and phage-encoded lytic enzymes are promising antimicrobial agents. In this study, we report the isolation identification of bacteriophage KP2025 from Klebsiella pneumoniae . Bioinformatics analysis revealed a putative endolysin, LysKP213, containing T4-like_lys domain. Purified LysKP213 was found to be highly thermostable, retaining approximately 44.4% its activity after 20 h incubation at 95°C, 57.5% residual 30 min 121°C. Furthermore, when administered in combination with polymyxin B or fused N-terminus peptide cecropin A (CecA), exhibited increased antibacterial against Gram-negative pathogens, including K. , Pseudomonas aeruginosa Acinetobacter baumannii Escherichia coli both vitro vivo These results indicated that is thermostable endolysin that, combined an outer membrane permeabilizer, has enhanced candidate agent for control infections by pathogens.

Language: Английский

Citations

5
Characterization of a phage endolysin LysLFP01 and its antibacterial activity DOI

Qiannan Wen,

Xuecheng Huang,

Wenxin Ma

et al.

International Journal of Food Microbiology, Journal Year: 2025, Volume and Issue: 432, P. 111110 - 111110

Published: Feb. 11, 2025

Language: Английский

Citations

0
Application of phage-derived enzymes for enhancing food safety DOI
Junhwan Kim, Xinyu Liao, Song Zhang

et al.

Food Research International, Journal Year: 2025, Volume and Issue: 209, P. 116318 - 116318

Published: March 19, 2025

Language: Английский

Citations

0
Phage Endolysins as an Alternative Biocontrol Strategy for Pathogenic and Spoilage Microorganisms in the Food Industry DOI Creative Commons
Maryoris Elisa Soto Lopez, Fernando A Mendoza-Corvis, Jose Jorge Salgado-Behaine

et al.

Viruses, Journal Year: 2025, Volume and Issue: 17(4), P. 564 - 564

Published: April 14, 2025

Food contamination by pathogenic and spoilage bacteria causes approximately 47 million cases of foodborne diseases in the United States leads to tons food spoilage, worsening loss situation worldwide. In addition, conventional preservation treatments implemented industry decrease food’s nutritional organoleptic quality. Therefore, there is a need for new alternatives counteract without altering its characteristics. Endolysins are promising strategy due their unique properties, such as host specificity, synergism with other antibacterial agents, mode action, low probability resistance development. These characteristics differentiate them from agents used industry. enzymes produced bacteriophages during process bacterial infection lysis. This review describes advances related endolysin application systems food, considering potential safety an overview conditions according type be controlled. We also highlight studies on encapsulation prolongation action time outbreaks that allow obtaining key information improve endolysins different matrices processing storage

Language: Английский

Citations

0
Isolation and Characterization of Virulent Bacteriophages and Controlling Salmonella Enteritidis Biofilms on Chicken Meat DOI
Xiaowen Sun,

Fan Xue,

Cong Cong

et al.

Microbial Pathogenesis, Journal Year: 2025, Volume and Issue: unknown, P. 107619 - 107619

Published: April 1, 2025

Language: Английский

Citations

0
Can natural preservatives serve as a new line of protective technology against bacterial pathogens in meat and meat products? DOI Creative Commons
Changyong Cheng, Lingli Jiang,

Xiaoliang Li

et al.

Food Quality and Safety, Journal Year: 2023, Volume and Issue: 8

Published: Oct. 19, 2023

Abstract Contamination of meats and meat products by pathogenic microorganisms is responsible for a significant percentage outbreaks foodborne illness. There are also concerns over the carcinogenic potential dietary nitrate nitrite in processed products. The past few decades have seen an extensive search novel technologies alternative to synthetic chemical preservatives reduce level contamination foods spoilage microbes. This review provides general overview natural with applications industry, including phages their endolysins, bacteriocins, microbial lipopeptides, antimicrobial peptides plant or insect origin, essential oils extracts origins. Instead providing summary data from published literature, we attempt elaborate challenges facing development as hurdles, taking into consideration sharp contrast between studies this particular field very limited industrial use. More specifically, emphasize great importance having streamlined approaches methodological guidelines research so that journey use safer could be shortened made easier.

Language: Английский

Citations

7
Isolation and Characterization of Two Novel Lytic Bacteriophages against Salmonella typhimurium and Their Biocontrol Potential in Food Products DOI Creative Commons
Yaxiong Song,

Wentao Gu,

Yaozhong Hu

et al.

Foods, Journal Year: 2024, Volume and Issue: 13(19), P. 3103 - 3103

Published: Sept. 28, 2024

Foodborne pathogens, such as Salmonella, are major factors that pose significant threats to global food safety and public health. Salmonella typhimurium is a prominent serotype contributing non-typhoidal salmonellosis, which prevalent foodborne illness affecting humans animals. Bacteriophages considered one of the most environmentally friendly biocontrol agents, particularly in industry, owing their high specificity safety. However, emergency phage-resistant mutants limits effect phage treatment, leading requirement for diversity lytic phages. Therefore, study isolated characterized two novel bacteriophages (SPYS_1 SPYS_2) targeting S. ATCC14028 evaluated effectiveness reducing contamination rates milk chicken tenders. Morphological genomic analyses indicated phages SPYS_1 SPYS_2 species classified under genus Skatevirus Berlinvirus, respectively. Both exhibited stability across broad range thermal pH conditions. The one-step growth curve result suggested both had short adsorption time large burst size single cycle. demonstrated noteworthy inhibition on milk, resulting ~2-log reduction within 2 4 h range. Overall, have shown potential application future.

Language: Английский

Citations

2
Characterization of phage HZY2308 against Acinetobacter baumannii and identification of phage-resistant bacteria DOI Creative Commons
Ruilin Wang, Xiaojuan You, Xinwei Liu

et al.

Virology Journal, Journal Year: 2024, Volume and Issue: 21(1)

Published: Nov. 7, 2024

Acinetobacter baumannii (AB) is a notable cause of hospital-acquired infections, with carbapenem-resistant (CRAB) classified as high-priority critical pathogen. Bacteriophage therapy emerging promising alternative to combat drug-resistant bacterial infections. In this study, lytic phage, HZY2308, was isolated from hospital sewage, and the biological properties, biosafety anti-biofilm properties phage HZY2308 were characterized identified. Moreover, antibacterial effect in combination antibiotics investigated, apparent characteristics phage-resistant strain AB48-R demonstrated, which provided data support for further studies elucidate mechanism generating resistance.

Language: Английский

Citations

2
Whole genome sequence analysis of Aeromonas-infecting bacteriophage AHPMCC7, a new species of genus Ahphunavirus and its application in Litopenaeus vannamei culture DOI Creative Commons

Smita Ghosh,

Priyanka Kar,

Sudipta Chakrabarti

et al.

Virology, Journal Year: 2023, Volume and Issue: 588, P. 109887 - 109887

Published: Sept. 27, 2023

Language: Английский

Citations

6
Bacteriophage endolysin Ply113 as a potent antibacterial agent against polymicrobial biofilms formed by enterococci and Staphylococcus aureus DOI Creative Commons
Jing Wang, Siyu Liang, Xiaofeng Lu

et al.

Frontiers in Microbiology, Journal Year: 2023, Volume and Issue: 14

Published: Dec. 12, 2023

Antibiotic resistance in Enterococcus faecium, faecalis, and Staphylococcus aureus remains a major public health concern worldwide. Furthermore, these microbes frequently co-exist biofilm-associated infections, largely nullifying antibiotic-based therapy. Therefore, it is imperative to develop an efficient therapeutic strategy for combating infections caused by polymicrobial biofilms. In this study, we investigated the antibacterial antibiofilm activity of bacteriophage endolysin Ply113 vitro. exhibited high rapid lytic against E. S. aureus, including vancomycin-resistant methicillin-resistant isolates. Transmission electron microscopy revealed that treatment led detachment bacterial cell walls considerable lysis. maintained stable over temperature range 4-45°C, pH 5.0-8.0, presence 0-400 mM NaCl. effectively eliminated mono-species biofilms formed dose-dependent manner. was also able eliminate dual-species faecium-S. faecalis-S. aureus. Additionally, exerted potent efficacy vivo, distinctly decreasing loads murine peritoneal septicemia model. Our findings suggest promising antimicrobial agent infections.

Language: Английский

Citations

6