KDM4B mutations in human cancers DOI Creative Commons

Wesley Bush,

Korey Bosart,

Renee A. Bouley

et al.

Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis, Journal Year: 2024, Volume and Issue: 829, P. 111866 - 111866

Published: June 12, 2024

Homologous recombination (HR) is essential for repair of DNA double-strand breaks (DSBs) and restart stalled or collapsed replication forks. Most cancers are characterized by mutations in components the DSB pathways. Redundant pathways exist eukaryotes from yeast to humans recent evidence has shown that complete loss HR function appears be lethal. Recent also cancer cells with one pathway can killed inhibiting more parallel pathways, a strategy currently aggressively explored as therapy. KDM4B histone demethylase pleiotropic functions, which participates preparing DSBs contributing chromatin remodeling. In this report we carried out pan-cancer analysis goal understanding their distribution interaction other genes. We find although co-occur genes, most not drivers pathogenic. A sequence conservation shows highly conserved residues resistant mutation. Finally, all occur heterozygous state. single mutation, R986L, was predicted significantly affect protein structure using computational modeling. This suggests makes contributions but key player.

Language: Английский

The androgen receptor interacts with GATA3 to transcriptionally regulate a luminal epithelial cell phenotype in breast cancer DOI Creative Commons
Leila Hosseinzadeh, Zoya Kikhtyak, Geraldine Laven‐Law

et al.

Genome biology, Journal Year: 2024, Volume and Issue: 25(1)

Published: Feb. 5, 2024

The androgen receptor (AR) is a tumor suppressor in estrogen (ER) positive breast cancer, role sustained some ER negative cancers. Key factors dictating AR genomic activity context are largely unknown. Herein, we employ an unbiased chromatin immunoprecipitation-based proteomic technique to identify endogenous interacting co-regulatory proteins and models of cancer gain new insight into mechanisms signaling this disease.

Language: Английский

Citations

9
Serine metabolism in tumor progression and immunotherapy DOI Creative Commons
Dong Huang, Hui Cai,

Hazel Huang

et al.

Discover Oncology, Journal Year: 2025, Volume and Issue: 16(1)

Published: April 28, 2025

Language: Английский

Citations

1
The enzymes of serine synthesis pathway in cancer metastasis DOI Creative Commons
Lie Li, Yuting Qin, Yuping Chen

et al.

Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, Journal Year: 2024, Volume and Issue: 1871(4), P. 119697 - 119697

Published: Feb. 19, 2024

Language: Английский

Citations

6
Identification of Eight Histone Methylation Modification Regulators Associated With Breast Cancer Prognosis DOI Creative Commons
Yanguang Cao, Xiaohui Li, Xingjie Chen

et al.

IET Systems Biology, Journal Year: 2025, Volume and Issue: 19(1)

Published: Jan. 1, 2025

ABSTRACT Histone methylation is an important epigenetic modification process coordinated by histone methyltransferases, demethylases and reader proteins plays a key role in the occurrence development of cancer. This study constructed risk scoring model around regulators conducted multidimensional comprehensive analysis to reveal its potential breast cancer prognosis drug sensitivity. First, 144 (HMMRs) were subjected differential univariate Cox regression analysis, nine differentially expressed HMMRs associated with survival screened out. Next, consisting eight was using LASSO algorithm, exhibiting independent predictive values training validation cohorts. Then, immune shows that patients high‐risk group divided has weakened response. In addition, through functional genes (DEGs) between low‐risk groups, we confirmed DEGs mainly affected nucleoplasm tumour microenvironment. Finally, sensitivity demonstrated our could be useful for screening identify drugs treating BRCA patients. conclusion, these may factors

Language: Английский

Citations

0
circBRAF promotes the progression of triple-negative breast cancer through modulating methylation by recruiting KDM4B to histone H3K9me3 and IGF2BP3 to mRNA DOI Open Access

Jing Lan

American Journal of Cancer Research, Journal Year: 2024, Volume and Issue: 14(5), P. 2020 - 2036

Published: Jan. 1, 2024

Understanding the molecular characteristics of triple-negative breast cancer (TNBC) and developing more tailored treatment approaches is crucial.Circular RNAs (circRNAs), as potential therapeutic targets, remain largely unexplored in TNBC.This study utilized circRNA microarray analysis to determine expression circRNAs TNBC, analyzing nine patient specimens.The circBRAF were examined using divergent PCR primers, Sanger sequencing, fluorescence situ hybridization (FISH) analysis, application RNase actinomycin D. The biological function TNBC was further investigated through colony formation, tube transwell assays.Crucially, mechanisms underlying effects on progression explored via RNA immunoprecipitation sequencing (RIP-seq) data, MS2 pulldown, (RNA-seq) knockdown, histone H3K9me3 modification, Chromatin Isolation by Purification (ChIRP) tests followed liquid chromatography-tandem mass spectrometry (LC-MS/MS).We focused particularly hsa_ circ_0007178, produced from exons 4-13 oncogene BRAF.Functional experiments revealed that crucial for development with its knockdown preventing angiogenesis, metastasis, cell division vitro.Mechanistically, interacts KDM4B IGF2BP3, promoting growth.Interaction IGF2BP3 increased VCAN, FN1, CDCA3, or B4GALT3 controlling mRNA stability N6-methyladenosine (m6A) modification.Furthermore, upregulated ADAMTS14 MMP9 recruitment enhance respective overexpression able overcome inhibitory siKDM4B siIGF2BP3 migration invasion.Our findings suggest may act an specific interactions implying could serve a potentially effective novel target TNBC.

Language: Английский

Citations

3
KDM4B mutations in human cancers DOI Creative Commons

Wesley Bush,

Korey Bosart,

Renee A. Bouley

et al.

Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis, Journal Year: 2024, Volume and Issue: 829, P. 111866 - 111866

Published: June 12, 2024

Homologous recombination (HR) is essential for repair of DNA double-strand breaks (DSBs) and restart stalled or collapsed replication forks. Most cancers are characterized by mutations in components the DSB pathways. Redundant pathways exist eukaryotes from yeast to humans recent evidence has shown that complete loss HR function appears be lethal. Recent also cancer cells with one pathway can killed inhibiting more parallel pathways, a strategy currently aggressively explored as therapy. KDM4B histone demethylase pleiotropic functions, which participates preparing DSBs contributing chromatin remodeling. In this report we carried out pan-cancer analysis goal understanding their distribution interaction other genes. We find although co-occur genes, most not drivers pathogenic. A sequence conservation shows highly conserved residues resistant mutation. Finally, all occur heterozygous state. single mutation, R986L, was predicted significantly affect protein structure using computational modeling. This suggests makes contributions but key player.

Language: Английский

Citations

2