miR-205-5p Modulates High Glucose-Induced VEGFA Levels in Diabetic Mice and ARPE-19 Cells DOI Creative Commons

María Ybarra,

Miriam Martínez-Santos, María Rosa Oltra

et al.

Antioxidants, Journal Year: 2025, Volume and Issue: 14(2), P. 218 - 218

Published: Feb. 14, 2025

High glucose levels may cause vascular alterations in patients with diabetes, which can lead to complications such as diabetic retinopathy-an abnormal growth of retinal blood vessels. The micro-RNA miR-205-5p is known regulate angiogenesis by modulating the expression endothelial factor (VEGFA) different systems. This study investigates role controlling VEGFA both vitro and eye under hyperglycemic conditions. An alloxan-induced mouse model pigment epithelium human cell line (ARPE-19) were exposed high treated an ectopic mimic. mRNA protein assessed using qRT-PCR, Western blot, immunocytochemistry. Additionally, umbilical vein cells (HUVECs) employed evaluate angiogenesis. Our results show that significantly reduced while upregulating ARPE-19 mice. administration (via transfection or intravitreal injection) restored inhibited HUVEC cultures. Based on these preliminary data, we suggest a potential therapeutic strategy against involving proliferative eye-related disorders.

Language: Английский

miR-205-5p Modulates High Glucose-Induced VEGFA Levels in Diabetic Mice and ARPE-19 Cells DOI Creative Commons

María Ybarra,

Miriam Martínez-Santos, María Rosa Oltra

et al.

Antioxidants, Journal Year: 2025, Volume and Issue: 14(2), P. 218 - 218

Published: Feb. 14, 2025

High glucose levels may cause vascular alterations in patients with diabetes, which can lead to complications such as diabetic retinopathy-an abnormal growth of retinal blood vessels. The micro-RNA miR-205-5p is known regulate angiogenesis by modulating the expression endothelial factor (VEGFA) different systems. This study investigates role controlling VEGFA both vitro and eye under hyperglycemic conditions. An alloxan-induced mouse model pigment epithelium human cell line (ARPE-19) were exposed high treated an ectopic mimic. mRNA protein assessed using qRT-PCR, Western blot, immunocytochemistry. Additionally, umbilical vein cells (HUVECs) employed evaluate angiogenesis. Our results show that significantly reduced while upregulating ARPE-19 mice. administration (via transfection or intravitreal injection) restored inhibited HUVEC cultures. Based on these preliminary data, we suggest a potential therapeutic strategy against involving proliferative eye-related disorders.

Language: Английский

Citations

0