In vitro models, Journal Year: 2023, Volume and Issue: 2(6), P. 307 - 315
Published: Dec. 7, 2023
Language: Английский
Neurological Research, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 12
Published: May 12, 2025
Ischemic stroke is a leading cause of mortality and disability worldwide, yet effective therapeutic options remain limited. In this study, bioinformatics analyses were used to identify potential targets small-molecule compounds for ischemic stroke. A mouse model cerebral ischemia was subsequently validate their neuroprotective efficacy. Bioinformatics methods analyze key signaling pathways hub genes associated with Additionally, the Connectivity Map (CMap) database queried treatment. Finally, middle artery occlusion/reperfusion (MCAO/R) employed further evaluate effects identified compounds. GO KEGG pathway enrichment revealed that such as TNF, NF-κB, IL-17 play crucial roles in PPI network analysis five genes-IL-1β, IL-6, ICAM-1, Jun, Fos-all closely neuroinflammatory responses. The compound PP2, selective Src kinase inhibitor, by CMap database. MCAO/R model, PP2 exhibited significant effects. It reduced infarct volume brain edema improved neurological function. Mechanistically, inhibited phosphorylation, thereby suppressing NF-κB reducing levels pro-inflammatory cytokines, including TNF-α, IL-1β, IL-6. This study identifies promising target highlights value drug discovery mechanistic research.
Language: Английский
Citations
0
Molecular Neurobiology, Journal Year: 2023, Volume and Issue: 60(12), P. 7118 - 7135
Published: Aug. 2, 2023
Language: Английский
Citations
9
Journal of Stroke and Cerebrovascular Diseases, Journal Year: 2024, Volume and Issue: 33(11), P. 107945 - 107945
Published: Aug. 29, 2024
Language: Английский
Citations
3
International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 25(1), P. 178 - 178
Published: Dec. 22, 2023
Ischemic stroke is one of the most significant causes morbidity and mortality worldwide. However, there a dearth effective drugs treatment methods for ischemic stroke. Significant numbers circular RNAs (circRNAs) exhibit abnormal expression following are considered potential therapeutic targets. CircRNAs have emerged as promising biomarkers due to their stable in peripheral blood significance diagnosis prognosis. This review provides summary 31 circRNAs involved pathophysiological processes apoptosis, autophagy, inflammation, oxidative stress, angiogenesis Furthermore, we discuss mechanisms action said clinical applications. Ultimately, promise both targets
Language: Английский
Citations
6
Journal of Biomedical Research, Journal Year: 2024, Volume and Issue: 38, P. 1 - 1
Published: Jan. 1, 2024
Most papillary thyroid carcinoma (PTC) patients have a good prognosis, but lymph node metastasis (LNM) is the most common progressive manifestation and often leads to poor-prognosis. However, few studies focused on underlying mechanisms of LNM. This study aimed identity potential role exosomal circRNAs that contribute LNM in PTC. We found 9000 aberrantly expressed PTC with LNM, including 684 observably upregulation 2193 notably downregulation. Functional enrichment analyses indicated these were mainly enriched variety molecules signaling pathways related progression Bioinformatics analysis screened 14 circRNA-miRNA-mRNA networks associated LNM-related Moreover, circTACC2-miR-7-EGFR circBIRC6-miR-24-3p-BCL2L11 axes verified for involvement Additionally, 4 upregulated circRNAs-related hub genes 8 downregulated screened, some which involved through verification. Collectively, our data provided novel framework in-depth investigation function dysregulated their biomarkers
Language: Английский
Citations
2
Brain Sciences, Journal Year: 2022, Volume and Issue: 12(8), P. 1000 - 1000
Published: July 28, 2022
Ischemic stroke is a life-threatening condition that also frequently results in long-term disability. Currently, intravenous thrombolysis with tissue plasminogen activator and mechanical thrombectomy the most popular treatment. However, narrow time window related complications limit treatment benefits. Exosomes have recently emerged as ideal therapeutic candidates for ischemic ability to pass through blood_brain barrier mediate intercellular communication, addition, exosomes their contents can be bioengineered implement targeted delivery. In last two decades, exosomal noncoding RNAs been found involved pathophysiological progression of stroke, including atherosclerosis, apoptosis, inflammation, oxidative stress, neurovascular remodeling. this review, we describe latest progress regarding role long circular occurrence, progression, recovery stroke. Exploration correlated effects may facilitate accurate diagnosis, they serve new targets disease.
Language: Английский
Citations
9
International Journal of Molecular Sciences, Journal Year: 2023, Volume and Issue: 24(14), P. 11325 - 11325
Published: July 11, 2023
Target biomarkers for H2 at both the protein and genome levels are still unclear. In this study, quantitative proteomics acquired from a mouse model were first analyzed. At same time, functional pathway analysis helped identify pathways level. Then, bioinformatics on mRNA sequencing data conducted between sepsis normal models. Differential expressional genes with closest relationship to disease status development identified through module correlation analysis. common in transcriptomics extracted as target biomarkers. Through analyzing expression trait locus (eQTL) genome-wide association studies (GWAS), colocalization Apoa2 phenotype was by summary-data-based Mendelian randomization (SMR). two-sample drug-target, syndrome (MR) analyses all using Twosample R package. For level, loci (pQTLs) of biomarker also included MR. Animal experiments validate these results. As result, or protective, causal sepsis. HDL type 2 diabetes proven possess relationships The agitation inhibition indicated influence related syndromes. conclusion, we proposed treatment.
Language: Английский
Citations
4
Journal of Personalized Medicine, Journal Year: 2023, Volume and Issue: 13(5), P. 839 - 839
Published: May 16, 2023
Despite the substantial improvement in diagnosis and treatment within last decades, ischemic stroke still represents a challenge, responsible for high burden of morbidity mortality. Among unmet clinical needs are difficulties identifying those subjects with greatest risk developing stroke, challenges obtaining timely diagnosis, prompt recognition different forms assessment response to treatments prognostic assessment. All these issues might be improved appropriate smart biomarkers that could better inform management. The present article offers an overview potential role circular RNAs as disease stroke. A systematic approach was adopted gather all potentially relevant information order provide panoramic view on this class promising molecules.
Language: Английский
Citations
3
Current Opinion in Lipidology, Journal Year: 2023, Volume and Issue: 34(2), P. 82 - 91
Published: March 2, 2023
Exosomes are lipid-bound particles that carry lipids, protein, and nucleic acid affect cellular function. This review highlights the current knowledge on crosstalk between exosomes lipid metabolism their impact cardiometabolic disease.Recent studies revealed lipids metabolizing enzymes important for exosome biogenesis internalization conversely how metabolism, secretion, degradation. The interplay affects disease pathophysiology. More importantly, might function as biomarkers diagnosis prognosis or possibly therapies.Recent advances in our understanding of have implications normal physiological functions well pathogenesis. Exosome novel diagnostic tests treatments disease.
Language: Английский
Citations
2
European Journal of Pharmacology, Journal Year: 2022, Volume and Issue: 939, P. 175477 - 175477
Published: Dec. 18, 2022
Language: Английский
Citations
3