Safflower Yellow Pigment Alleviates Cerebral Ischemia‐Reperfusion Injury via Protein Nitration and Oxidative Modulation
Brain and Behavior,
Journal Year:
2025,
Volume and Issue:
15(5)
Published: May 1, 2025
ABSTRACT
Objective
We
seek
to
investigate
the
efficacy
of
safflower
yellow
pigment
in
mitigating
cerebral
ischemia‐reperfusion
injury
by
examining
its
effects
on
protein
nitration
and
oxidative
modification.
Methods
A
total
160
patients
with
acute
ischemic
stroke
admitted
department
neurology
Xingtai
people's
hospital
were
included
this
study.
This
study
was
a
retrospective
Patients
divided
into
control
group
(
n
=
80)
observation
according
whether
used.
The
received
conventional
treatment,
pigment.
Results
Baseline
characteristics
did
not
significantly
differ
between
two
groups
p
>
0.05).
Serum
nitrosine
levels
lower
compared
after
24
h
one
week
treatment
<
Similarly,
serum
carbonylated
exhibited
NIHSS
modified
rankin
scale
(mRS)
scores,
reduced
area.
Furthermore,
malondialdehyde
(MDA),
C‐reactive
(CRP),
tumor
necrosis
factor‐alpha
(TNF‐α)
lower,
while
superoxide
dismutase
(SOD)
activity
higher
Conclusion
Safflower
demonstrates
significant
neuroprotective
reducing
oxidation
nitration,
improving
neurological
function,
area,
attenuating
stress
inflammation.
Language: Английский
Non-coding RNAs in acute ischemic stroke: from brain to periphery
Neural Regeneration Research,
Journal Year:
2024,
Volume and Issue:
20(1), P. 116 - 129
Published: March 1, 2024
Acute
ischemic
stroke
is
a
clinical
emergency
and
condition
with
high
morbidity,
mortality,
disability.
Accurate
predictive,
diagnostic,
prognostic
biomarkers
effective
therapeutic
targets
for
acute
remain
undetermined.
With
innovations
in
high-throughput
gene
sequencing
analysis,
many
aberrantly
expressed
non-coding
RNAs
(ncRNAs)
the
brain
peripheral
blood
after
have
been
found
samples
experimental
models.
Differentially
ncRNAs
post-stroke
were
demonstrated
to
play
vital
roles
pathological
processes,
leading
neuroprotection
or
deterioration,
thus
can
serve
as
stroke.
Moreover,
distinctly
be
used
prediction,
diagnosis,
prognosis.
In
particular,
immune
cells
recently
shown
involved
response
this
review,
we
consolidate
latest
progress
of
research
into
(microRNAs,
long
ncRNAs,
circular
RNAs)
processes
stroke-induced
damage,
well
potential
these
act
Findings
from
review
will
provide
novel
ideas
application
Language: Английский
BONE MARROW MESENCHYMAL STROMAL CELL-DERIVED EXOSOMAL NRF2 AMELIORATES CEREBRAL ISCHEMIA-REPERFUSION INJURY BY TRANSCRIPTIONALLY ACTIVATING LIN28A
Dongwen Liu,
No information about this author
Chunyan Song,
No information about this author
Cuiling Lv
No information about this author
et al.
Shock,
Journal Year:
2024,
Volume and Issue:
62(1), P. 85 - 94
Published: March 25, 2024
ABSTRACT
Background:
Cerebral
ischemia-reperfusion
(I/R)
injury
(CIRI)
have
severe
consequences
on
brain
function,
and
the
exciting
evidence
has
revealed
protective
role
of
acyl-CoA
synthetase
long
chain
family
member
4
(Lin28a)
against
cerebral
injury.
The
present
work
aims
to
reveal
its
molecular
mechanism
in
regulating
CIRI,
with
hope
providing
a
therapeutic
method
for
I/R
We
hypothesized
that
exosomal
nuclear
factor
erythroid
2-related
2
(NRF2)
derived
from
bone
marrow
mesenchymal
stromal
cells
could
transcriptionally
activate
Lin28a
thereby
alleviate
This
hypothesis
was
validated
work.
Methods:
Middle
artery
occlusion
(MCAO)
model
established
using
C57BL/6J
mice,
neurological
deficit,
infarct
volume,
water
content
were
assessed
evaluate
neuron
Human
glioblastoma
(A172)
subjected
oxygen-glucose
deprivation
reoxygenation
(OGD/R)
treatment
mimic
cell
model.
Exosome
isolation
reagent
used
isolate
exosomes
supernatant
through
sequential
centrifugation
filtration
steps.
mRNA
expression
level
detected
by
quantitative
real-time
polymerase
reaction.
Protein
analyzed
western
blotting
assay.
TUNEL
apoptosis
detection
kit
analyze
tissues.
Enzyme-linked
immunosorbent
assays
commercial
kits
detect
levels
inflammatory
markers
oxidative
stress
markers.
Ferrous
Iron
Colorimetric
Assay
Kit
Fe
2+
colorimetric
assay
level.
association
NRF2
identified
chromatin
immunoprecipitation
dual-luciferase
reporter
Results:
MCAO
substantially
augmented
volume
impaired
elevated
content.
lowly
expressed
tissues
mice
overexpression
protected
mice.
Moreover,
A172
OGD/R
treatment-induced
Additionally,
activated
cells.
Bone
cell-derived
increased
NRF2-dependent
manner.
improved
OGD/R-induced
inducing
production.
Conclusion:
CIRI
activating
Lin28a.
Language: Английский
Extracellular vesicles for delivering therapeutic agents in ischemia/reperfusion injury
Weihang Zhou,
No information about this author
Xinchi Jiang,
No information about this author
Jianqing Gao
No information about this author
et al.
Asian Journal of Pharmaceutical Sciences,
Journal Year:
2024,
Volume and Issue:
19(6), P. 100965 - 100965
Published: Sept. 4, 2024
Ischemia/reperfusion
(I/R)
injury
is
marked
by
the
restriction
and
subsequent
restoration
of
blood
supply
to
an
organ.
This
process
can
exacerbate
initial
tissue
damage,
leading
further
disorders,
disability,
even
death.
Extracellular
vesicles
(EVs)
are
crucial
in
cell
communication
releasing
cargo
that
regulates
physiological
state
recipient
cells.
The
development
EVs
presents
a
novel
avenue
for
delivering
therapeutic
agents
I/R
therapy.
potential
derived
from
stem
cells,
endothelial
plasma
has
been
actively
investigated.
Therefore,
this
review
aims
provide
overview
pathological
biophysical
properties
EVs.
We
noted
serve
as
nontoxic,
flexible,
multifunctional
carriers
capable
intervening
progression.
efficacy
be
enhanced
through
various
engineering
strategies.
Improving
tropism
Language: Английский