European Journal of Histochemistry, Journal Year: 2025, Volume and Issue: 69(1)

Published: March 12, 2025

Accurate differentiation of homologous proteins that share high sequence identity remains a significant challenge in biomedical research, as conventional antibodies often lack sufficient specificity, leading to potential misinterpretations. This issue is particularly evident the study hexokinases, family isoenzymes catalyze first step glycolysis by phosphorylating glucose. Beyond their canonical metabolic roles, hexokinases play critical non-glycolytic functions, especially cancer biology. However, unique tissue distributions and context-dependent roles are obscured overlapping specificities commercially available antibodies, which can produce misleading results. In this study, we rigorously evaluated panel targeting hexokinase isoenzyme 3 (HK3), highlighting widespread cross-reactivity insufficient validation. Through process, identified validated highly specific antibody for HK3, demonstrating its reliability western blot immunohistochemistry applications. Using tool, reveal distinct localization HK3 myeloid cell populations, providing new insights into functional these cells. work addresses gap specificity establishes uniquely expressed gene immune cells absent other types under basal conditions. Providing foundation future investigations functions.

Language: Английский