Edaravone Alleviates BV‐2 Microglia‐Mediated Neuroinflammation Through the PI3K/AKT/ NF‐κB Pathway

Advanced Biology, Journal Year: 2025, Volume and Issue: unknown

Published: April 8, 2025

Abstract Ischemic stroke (IS) poses a significant threat to human health. Research has demonstrated that microglia (MG)‐mediated neuroinflammatory responses play crucial role in the pathogenesis of IS. Consequently, inhibiting MG activation and reducing inflammatory response may be key strategies for clinical treatment neurodegenerative diseases. Edaravone (EDA), potent anti‐inflammatory antioxidant, is currently used IS; however, its mechanisms remain inadequately understood. To address this, network pharmacology (NP) analysis employed identify phosphatidylinositol 3‐kinase/protein kinase B (PI3K/Akt) signaling pathway as potential mediator triggered by activated following EDA treatment. In vitro oxygen‐glucose deprivation (OGD) induce BV‐2 activation, an vivo middle cerebral artery occlusion (MCAO) mouse model established. Western blot immunofluorescence staining are detect changes phosphorylation levels pathway‐related proteins expression factors. Additionally, PI3K inhibitor LY294002 overexpression plasmid introduced further analyze these markers. The results suggest alleviate mediated through PI3K/Akt pathway.

Language: Английский

The Role of Copper Homeostasis and Cuproptosis in Cerebrovascular Diseases :A novel Therapeutic Target

European Journal of Pharmacology, Journal Year: 2025, Volume and Issue: unknown, P. 177649 - 177649

Published: May 1, 2025

Language: Английский