Addressing the Knowledge Deficit in Hospital Bed Planning, Defining An Optimum Region for the Number of Different Types of Hospital Beds in An Effective Health Care System DOI Open Access
Rod Jones

Published: July 11, 2023

. The number of hospital beds per 1000 population is commonly used to compare international bed numbers. This method flawed because it does not consider age structure or the effect nearness-to-death on utilization. Deaths are also serving as a proxy for wider demand arising from undetected outbreaks 3 000 species human pathogens. To remedy this problem new approach modelling has been developed which plots 1 deaths against population. Lines equivalence can be drawn plot delineate countries with higher lower supply. extended attempt define optimum region supply in an effective health care system. England example system descending into operational chaos due too few and manpower. former Soviet bloc represent overly dependent beds. Several show evidence over-utilization potential range manpower where current most systems currently reside. applied total curative beds, medical psychiatric critical care, geriatric etc., different types healthcare staff, i.e., nurses, physicians, surgeons. Issues surrounding size average occupancy will discussed. role poor policy English NHS how led crisis.

Language: Английский

The miRNomics of antiretroviral therapy-induced obesity DOI Creative Commons

Niska Majumdar,

Bishwa Raj Pokharel,

Anne E. Dickerson

et al.

Functional & Integrative Genomics, Journal Year: 2025, Volume and Issue: 25(1)

Published: April 5, 2025

Language: Английский

Citations

0
MicroRNA transcriptome analysis for post-mortem interval estimation DOI
Haneul Lee,

Eun Ju Lee,

Kwangmin Park

et al.

Forensic Science International, Journal Year: 2025, Volume and Issue: 370, P. 112473 - 112473

Published: April 12, 2025

Language: Английский

Citations

0
miR-182-5p facilitates colorectal cancer progression through manipulating neurocalcin delta mediated Wnt/β-catenin signalling DOI Creative Commons
Pengfei Wang, Gang Li,

Xianglin Sun

et al.

European journal of medical research, Journal Year: 2025, Volume and Issue: 30(1)

Published: May 2, 2025

Colorectal cancer (CRC), a complex and multifactorial disease, has been associated with elevated expression of microRNA miR-182-5p, although its precise regulatory role in CRC progression remains unclear. This study aims to identify potential therapeutic targets improve clinical outcomes decipher the intricate miR-182-5p pathobiology CRC. We conducted comprehensive bioinformatics analyses using GEO databases investigate differences miRNA between normal tissues, particular focus on miR-182-5p. Its levels cells tumor tissues were quantified by quantitative real-time PCR (qRT-PCR). The neurocalcin delta (NCALD) proteins related Wnt/β-catenin signalling was evaluated qRT-PCR Western blotting. Pathological changes tumor-bearing mice as well proliferation, invasion, migration cells, assessed. Tumor cell proliferation apoptosis examined Ki-67 immunohistochemistry TUNEL staining, respectively. A dual luciferase reporter assay explored interaction NCALD. Our findings reveal significantly lines, positively correlated invasion depth, differentiation degree, stage, lymph node metastasis. appears accelerate both lines mouse models downregulating NCALD, thereby enhancing signalling. identifies pivotal enhancer progression, modulating via NCALD regulation. position miR-182-5p/NCALD axis promising for therapy, offering new avenues treatment strategies. Retrospectively registered.

Language: Английский

Citations

0
Ionic Strength Investigation on the Interaction Between miR-155 and a PNA-Based Probe by Atomic Force Spectroscopy DOI Creative Commons
Davide Atzei, Francesco Lavecchia di Tocco, Anna Rita Bizzarri

et al.

Biomolecules, Journal Year: 2025, Volume and Issue: 15(5), P. 634 - 634

Published: April 28, 2025

Peptide nucleic acids (PNAs) are synthetic analogues of DNA/RNA characterized by the absence negative phosphate groups, which confer a low sensitivity to ionic strength for hybridization with respect canonical counterpart. PNAs suitable probe miRNAs, as well endogenous molecules single-strand non-coding RNA whose dysregulation is often linked several diseases. The interaction forces between PNA and microRNA-155 (miR-155), multifunctional microRNA overexpressed in variety tumors, were investigated Atomic Force Spectroscopy (AFS) fluid under different conditions. We found that unbinding acquired at 150 mM rather wide range loading rates (ΔF/Δt) can be described using Bell–Evans model. This allows us extract information on kinetics thermodynamic properties miR-155/PNA duplex. Additionally, we probed target recognition times miR-155 50–300 range. Our results indicate both these parameters practically independent from analyzed provide useful wider use biosensors diagnostics therapeutics, even situ.

Language: Английский

Citations

0
RNA–DNA Differences: Mechanisms, Oxidative Stress, Transcriptional Fidelity, and Health Implications DOI Creative Commons

Viktor Štolc,

Ondrej Preto,

Miloslav Karhánek

et al.

Antioxidants, Journal Year: 2025, Volume and Issue: 14(5), P. 544 - 544

Published: April 30, 2025

RNA–DNA differences (RDDs) challenge the traditional view of RNA as a faithful copy DNA, arising through editing, transcriptional errors, and oxidative damage. Reactive oxygen species (ROS) play central role, inducing lesions like 8-oxo-guanine that compromise transcription translation, leading to dysfunctional proteins. This review explores biochemical basis RDDs, their exacerbation under stress, dual roles in cellular adaptation disease. RDDs contribute genomic instability are implicated cancers, neurodegenerative disorders, autoimmune diseases, while also driving phenotypic diversity. Drawing on terrestrial spaceflight studies, we highlight intersection RDD formation, dysfunction, proposing innovative mitigation approaches. Advancements detection quantification, along with ROS management therapies, offer new avenues restore homeostasis promote resilience. By positioning hallmark entropy, this underscores limits biological adaptation. Furthermore, prevalence guanine-rich codons antioxidant genes increases susceptibility ROS-induced lesions, linking redox instability, constrained These insights have profound implications for understanding aging, disease progression, adaptive mechanisms both space environments.

Language: Английский

Citations

0
Non-coding RNAs: MicroRNAs (miRNAs) DOI
Phei Er Saw,

Erwei Song

Published: Jan. 1, 2025

Language: Английский

Citations

0
The comparison of miRNA expression levels in Type 2 diabetes and diabetic peripheral neuropathy patients DOI
Aylin Ayaz, Sibel Kuraş, Bülent Erdoğan

et al.

Clinical Biochemistry, Journal Year: 2025, Volume and Issue: unknown, P. 110946 - 110946

Published: May 1, 2025

Language: Английский

Citations

0
Macrophage dynamics in prostate cancer: Molecular to therapeutic insights DOI Open Access

Qiannan Gu,

Anning Qi,

Ne Wang

et al.

Biomedicine & Pharmacotherapy, Journal Year: 2024, Volume and Issue: 177, P. 117002 - 117002

Published: July 2, 2024

Language: Английский

Citations

2
Addressing the Knowledge Deficit in Hospital Bed Planning and Defining an Optimum Region for the Number of Different Types of Hospital Beds in an Effective Health Care System DOI Open Access
Rod Jones

International Journal of Environmental Research and Public Health, Journal Year: 2023, Volume and Issue: 20(24), P. 7171 - 7171

Published: Dec. 12, 2023

Based upon 30-years of research by the author, a new approach to hospital bed planning and international benchmarking is proposed. The number beds per 1000 people commonly used compare numbers. This method flawed because it does not consider population age structure or effect nearness-to-death on utilization. Deaths are also serving as proxy for wider demand arising from undetected outbreaks 3000 species human pathogens. To remedy this problem, modeling has been developed that plots deaths against population. Lines equivalence can be drawn plot delineate countries with higher lower supply. extended attempt define optimum region supply in an effective health care system. England example system descending into operational chaos due too few manpower. former Soviet bloc represent overly dependent beds. Several show evidence overutilization potential range manpower where most systems currently reside. applied total curative beds, medical psychiatric critical care, geriatric etc., different types healthcare staff, i.e., nurses, physicians, surgeons. Issues surrounding size average occupancy will discussed. role poor policy English NHS how led crisis. beyond illustrate at local regional level process long-term planning. regarding volatility admissions addressed explain need surge capacity why adequate margin required optimally functioning hospital.

Language: Английский

Citations

6
The Emerging Role of MicroRNAs and Autophagy Mechanism in Pancreatic Cancer Progression: Future Therapeutic Approaches DOI Open Access
Evangelos Koustas, Eleni-Myrto Trifylli, Panagiotis Sarantis

et al.

Genes, Journal Year: 2022, Volume and Issue: 13(10), P. 1868 - 1868

Published: Oct. 15, 2022

Pancreatic cancer constitutes the fourth most frequent cause of death due to malignancy in US. Despite new therapeutic modalities, management pancreatic ductal adenocarcinoma (PDAC) is considered a difficult task for clinicians fact that usually diagnosed already advanced stages and it relatively resistant current chemotherapeutic agents. The molecular background analysis malignant tumors, which includes various epigenetic genetic alterations, opens horizons development novel diagnostic strategies. interplay between miRNAs, autophagy pathway, carcinogenesis spotlight research. There strong evidence miRNAs take part either as tumor inhibitors combat oncogene expression or promoters (oncomiRs) by acting oncogenes interfering with cell functions such proliferation, programmed death, metabolic signaling pathways. Deregulation levels closely associated growth, progression, dissemination, well low sensitivity Similarly, despite constituting pivotal homeostatic mechanism survival has binary role PDAC, an inhibitor promoter carcinogenesis. emerging gets great deal attention opportunities strategies this aggressive chemoresistant malignancy. In review, we will shed light on progression.

Language: Английский

Citations

8