Novel Insights into Phytoplasma Effectors DOI Creative Commons
Karla Gisel Carreón-Anguiano,

Sara Elena Vila-Luna,

Luis Sáenz-Carbonell

et al.

Horticulturae, Journal Year: 2023, Volume and Issue: 9(11), P. 1228 - 1228

Published: Nov. 14, 2023

Effectoromics has become integral to the identification of pathogen targets and/or host-resistant proteins for genetic improvement plants in agriculture and horticulture. Phytoplasmas are causal agents more than 100 plant diseases economically important crops such as vegetables, spices, medicinal plants, ornamentals, palms, fruit trees, etc. To date, around 20 effectors phytoplasmas have been experimentally validated but list putative comprises hundreds different proteins. Very few families (tribes) identified based on homology, SAP05-like, SAP11-like, SAP54-like TENGU-like families. The lack conservation amino acid sequences slows progress effectoromics since many must be studied individually. Here, 717 phytoplasma effector candidates 21 were characterized silico identify common features. We functional domains 153 effectors, while 585 had no known domains. most frequently domain was sequence-variable mosaic (SVM domain), widely distributed 87 effectors. Searching de novo motifs, 50 found dataset; 696 at least 1 motif 42 all. These data allowed us organize into 15 tribes, uncovering, first time, evolutionary relationships largely masked by sequence among also eukaryotic linear motifs (ELMs) sequences. Since related functions, this novel organization may help further advance research combat infection

Language: Английский

ELM—the Eukaryotic Linear Motif resource—2024 update DOI Creative Commons
Manjeet Kumar, Sushama Michael, Jesús Alvarado-Valverde

et al.

Nucleic Acids Research, Journal Year: 2023, Volume and Issue: 52(D1), P. D442 - D455

Published: Nov. 14, 2023

Abstract Short Linear Motifs (SLiMs) are the smallest structural and functional components of modular eukaryotic proteins. They also most abundant, especially when considering post-translational modifications. As well as being found throughout cell part regulatory processes, SLiMs extensively mimicked by intracellular pathogens. At heart Eukaryotic Motif (ELM) Resource is a representative (not comprehensive) database. The ELM entries created growing community skilled annotators provide an introduction to linear motif functionality for biomedical researchers. 2024 update includes 346 novel instances in areas ranging from innate immunity both protein RNA degradation systems. In total, 39 classes newly annotated motifs have been added, another 17 existing updated release now 356 incorporating 4283 individual manually curated 4274 scientific publications including >700 links experimentally determined 3D structures. recent development, InterPro module resource data. available at: http://elm.eu.org.

Language: Английский

Citations

53
Global Impact of hMPV Virus: Transmission, Pathogenesis, Diagnostic and Treatment DOI

S Porwal,

Rishabha Malviya, Sathvik Belagodu Sridhar

et al.

Diagnostic Microbiology and Infectious Disease, Journal Year: 2025, Volume and Issue: unknown, P. 116809 - 116809

Published: March 1, 2025

Language: Английский

Citations

0
How different viruses perturb host cellular machinery via short linear motifs. DOI

Sobia Idrees,

Keshav Raj Paudel,

Tayyaba Sadaf

et al.

PubMed, Journal Year: 2023, Volume and Issue: 22, P. 1113 - 1128

Published: Jan. 1, 2023

The virus interacts with its hosts by developing protein-protein interactions. Most viruses employ protein interactions to imitate the host protein: A viral same amino acid sequence or structure as attaches protein's binding partner and interferes pathways. Being opportunistic, have evolved manipulate cellular mechanisms mimicking short linear motifs. In this review, we shed light on current understanding of mimicry via motifs focus genetically different subtypes providing recent examples evidence how high-throughput methods can be a reliable source study SLiM-mediated mimicry.

Language: Английский

Citations

7
Prediction of motif-mediated viral mimicry through the integration of host–pathogen interactions DOI Creative Commons
Sobia Idrees, Keshav Raj Paudel, Philip M. Hansbro

et al.

Archives of Microbiology, Journal Year: 2024, Volume and Issue: 206(3)

Published: Feb. 9, 2024

Abstract One of the mechanisms viruses use in hijacking host cellular machinery is mimicking Short Linear Motifs (SLiMs) proteins to maintain their life cycle inside cells. In face escalating volume virus-host protein–protein interactions (vhPPIs) documented databases; accurate prediction molecular mimicry remains a formidable challenge due inherent degeneracy SLiMs. Consequently, there pressing need for computational methodologies predict new instances viral mimicry. Our present study introduces DMI- de-novo pipeline, revealing that vhPPIs catalogued VirHostNet3.0 database effectively capture domain-motif (DMIs). Notably, both affinity purification coupled mass spectrometry and yeast two-hybrid assays emerged as good approaches delineating DMIs. Furthermore, we have identified mediated by SLiMs across different viruses. Importantly, strategy facilitated recognition several potential candidates implicated subversion proteins. The insights gleaned from this research not only enhance our comprehension which co-opt but also pave way development novel therapeutic interventions.

Language: Английский

Citations

2
Novel Insights into Phytoplasma Effectors DOI Creative Commons
Karla Gisel Carreón-Anguiano,

Sara Elena Vila-Luna,

Luis Sáenz-Carbonell

et al.

Horticulturae, Journal Year: 2023, Volume and Issue: 9(11), P. 1228 - 1228

Published: Nov. 14, 2023

Effectoromics has become integral to the identification of pathogen targets and/or host-resistant proteins for genetic improvement plants in agriculture and horticulture. Phytoplasmas are causal agents more than 100 plant diseases economically important crops such as vegetables, spices, medicinal plants, ornamentals, palms, fruit trees, etc. To date, around 20 effectors phytoplasmas have been experimentally validated but list putative comprises hundreds different proteins. Very few families (tribes) identified based on homology, SAP05-like, SAP11-like, SAP54-like TENGU-like families. The lack conservation amino acid sequences slows progress effectoromics since many must be studied individually. Here, 717 phytoplasma effector candidates 21 were characterized silico identify common features. We functional domains 153 effectors, while 585 had no known domains. most frequently domain was sequence-variable mosaic (SVM domain), widely distributed 87 effectors. Searching de novo motifs, 50 found dataset; 696 at least 1 motif 42 all. These data allowed us organize into 15 tribes, uncovering, first time, evolutionary relationships largely masked by sequence among also eukaryotic linear motifs (ELMs) sequences. Since related functions, this novel organization may help further advance research combat infection

Language: Английский

Citations

4