Separate and Combined Effects of Moderate-Intensity Exercise Training and Detraining with Protocatechuic Acid (PCA) on Myokines and Insulin-Signaling Pathways in Male Wistar Rats: A Preclinical Randomized Study
Metabolites,
Journal Year:
2025,
Volume and Issue:
15(2), P. 87 - 87
Published: Feb. 1, 2025
Background:
Exercise
training
positively
modulates
myokine
secretion
and
improves
glucose
metabolism.
Herein,
we
analyzed
the
effect
of
moderate-intensity
training,
detraining,
Protocatechuic
Acid
(PCA)
supplementation
on
secretions
regulation
insulin-signaling
pathways.
Methods:
A
five-arm
study
was
conducted
47
healthy
male
Wistar
rats,
trained
at
a
moderate
intensity
level
for
four
weeks
(T0-T4).
Animals
were
randomly
classified
into
groups
according
to
PCA
exercise
durations:
Aerobic
Training
with
or
without
(AT4,
AT4-PCA),
eight
(AT8,
AT8-PCA),
Vehicle
Control
(VC).
The
animals
followed
up
until
week
12
(T12).
We
decapitated
six
rats
T0
T4,
per
group
T8,
three
T12.
Myokines
(IGF-1,
IL-6,
FGF-21,
myostatin,
irisin)
ELISA.
Western
blot
analysis
measured
protein
expression
pathways
GLUT-4
in
gastrocnemius
muscle.
Results:
IL-6
levels
increased
significantly
(p
<
0.01)
8-week
AT8
by
34%
AT8-PCA
32%,
compared
only
4
(AT4
AT4-PCA).
Similarly,
PI3K,
improved
T8.
IGF-1
levels,
but
further
14%
improvement
observed
Myostatin
dropped
27%
even
4-week
0.001).
However,
detraining
myostatin
all
groups,
dose,
reduced
11%
FGF-21
54%
during
T12
AT8.
irisin
did
not
change
markedly
any
group.
Conclusions:
Physical
(with
PCA)
production
metabolism,
benefits
are
lost
after
detraining.
Language: Английский
Pre-clinical evidence for mitochondria as a therapeutic target for luteolin: a mechanistic view
Chemico-Biological Interactions,
Journal Year:
2025,
Volume and Issue:
unknown, P. 111492 - 111492
Published: March 1, 2025
Language: Английский
Measurement of blood pressure in rats: Invasive or noninvasive methods?
Physiological Reports,
Journal Year:
2024,
Volume and Issue:
12(17)
Published: Sept. 1, 2024
Abstract
Experiments
should
always
be
based
on
control
values.
This
assumption
fully
applies
to
cardiovascular
parameters,
such
as
heart
rate
(HR)
and
blood
pressure
(BP),
which
are
highly
sensitive
various
external
internal
stimuli
can
already
significantly
altered
when
an
experiment
begins.
Therefore,
it
is
necessary
determine
values
defined
a
starting
point
(i.e.,
baseline)
or
compare
them
with
valid
reference
if
the
goal
evaluate
changes
after
experimental
intervention.
A
generally
accepted
principle
reciprocal
relationship
between
BP
HR,
in
one
parameter
affects
other
vice
versa.
measured
using
two
methods—noninvasively
(tail‐cuff)
invasively
(telemetry,
direct
measurements
of
introducing
sensor
directly
into
artery),
HR
by
extrapolation
from
recordings.
study
does
not
aim
results
individual
studies,
but
review
whether
there
differences
(baseline)
normotensive
hypertensive
male
rats
invasive
versus
noninvasive
methods,
investigate
causal
vivo
experiments
rats.
Language: Английский