M2 macrophage-derived exosomes reverse TGF-β1-induced epithelial mesenchymal transformation in BEAS-2B cells via the TGF-βRI/Smad2/3 signaling pathway DOI Creative Commons
Chao Liu, Xiaolin Huang, Siqi Li

et al.

European journal of medical research, Journal Year: 2025, Volume and Issue: 30(1)

Published: April 11, 2025

Airway remodeling in bronchial asthma can be inhibited by disrupting the epithelial mesenchymal transition (EMT) of activated airway cells. Exosomes, as key mediators intercellular communication, have been implicated pathophysiology asthma-related inflammation, remodeling, and hyperresponsiveness. This study aimed to investigate role M2 macrophage-derived exosomes (M2φ-exos) modulating TGF-β1-induced EMT (BEAS-2B) cells elucidate underlying molecular mechanism, if any. THP-1 were induced differentiate into macrophages via phorbol 12-myristate 13-acetate (PMA) IL-4. Exosomes subsequently isolated purified ultracentrifugation. M2φ-exos expression was characterized protein marker levels, transmission electron microscopy imaging, nanoparticle tracking analysis. BEAS-2B exposed determine latter's effects. successfully differentiated macrophages, confirmed vitro flow cytometry. The presented typical cup-shaped structures expressed CD81 TSG101. TGF-β1 induction altered morphological characteristics TGF-βRI/Smad2/3 signaling pathway, leading increased Snail, Vimentin Collagen 1 decreased E-cadherin. After exosome or SB431542 induction, reversed. GW4869, an release inhibitor, exhibited ability block beneficial effects exosomes. M2Φ-exos through pathway. novel insight may important implications for asthma, particularly addressing remodeling.

Language: Английский

Phytochemical-mediated modulation of signaling pathways: A promising avenue for drug discovery DOI Creative Commons

Jibon Kumar Paul,

Mahir Azmal,

Anm Shah Newaz Been Haque

et al.

Advances in Redox Research, Journal Year: 2024, Volume and Issue: 13, P. 100113 - 100113

Published: Aug. 31, 2024

Language: Английский

Citations

9
Evaluating the role of targeted silymarin loaded hyaluronic acid/protein nanoparticles in activating hepatic progenitor stem cells for liver regeneration after CCl4-induced liver damage DOI

Rawan O El-Belkasy,

Maged El‐Kemary,

Nemany A.N. Hanafy

et al.

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: unknown, P. 142837 - 142837

Published: April 1, 2025

Language: Английский

Citations

0
M2 macrophage-derived exosomes reverse TGF-β1-induced epithelial mesenchymal transformation in BEAS-2B cells via the TGF-βRI/Smad2/3 signaling pathway DOI Creative Commons
Chao Liu, Xiaolin Huang, Siqi Li

et al.

European journal of medical research, Journal Year: 2025, Volume and Issue: 30(1)

Published: April 11, 2025

Airway remodeling in bronchial asthma can be inhibited by disrupting the epithelial mesenchymal transition (EMT) of activated airway cells. Exosomes, as key mediators intercellular communication, have been implicated pathophysiology asthma-related inflammation, remodeling, and hyperresponsiveness. This study aimed to investigate role M2 macrophage-derived exosomes (M2φ-exos) modulating TGF-β1-induced EMT (BEAS-2B) cells elucidate underlying molecular mechanism, if any. THP-1 were induced differentiate into macrophages via phorbol 12-myristate 13-acetate (PMA) IL-4. Exosomes subsequently isolated purified ultracentrifugation. M2φ-exos expression was characterized protein marker levels, transmission electron microscopy imaging, nanoparticle tracking analysis. BEAS-2B exposed determine latter's effects. successfully differentiated macrophages, confirmed vitro flow cytometry. The presented typical cup-shaped structures expressed CD81 TSG101. TGF-β1 induction altered morphological characteristics TGF-βRI/Smad2/3 signaling pathway, leading increased Snail, Vimentin Collagen 1 decreased E-cadherin. After exosome or SB431542 induction, reversed. GW4869, an release inhibitor, exhibited ability block beneficial effects exosomes. M2Φ-exos through pathway. novel insight may important implications for asthma, particularly addressing remodeling.

Language: Английский

Citations

0