Genome sequencing of Escherichia coli phage UFJF_EcSW4 reveals a novel lytic Kayfunavirus species DOI
Pedro Marcus Pereira Vidigal, Humberto Moreira Húngaro

3 Biotech, Journal Year: 2024, Volume and Issue: 15(1)

Published: Dec. 15, 2024

Language: Английский

From whole genomes to probiotic candidates: A study of potential lactobacilli strains selection for vaginitis treatment DOI Creative Commons

Jinli Lyu,

Mengyu Gao,

Shaowei Zhao

et al.

Heliyon, Journal Year: 2024, Volume and Issue: 10(9), P. e30495 - e30495

Published: April 30, 2024

Vaginitis, characterized by pathogenic invasion and a deficiency in beneficial lactobacilli, has recognized lactobacilli supplementation as novel therapeutic strategy. However, due to individual differences vaginal microbiota, identifying universally effective Lactobacillus strains is challenging. Traditional methodologies for probiotic selection, which heavily depend on extensive vitro experiments, are both time-intensive laborious. The aim of this study was pinpoint possible candidates based whole-genome screening. We sequenced the genomes 98 previously isolated strains, annotating their genes involved metabolite biosynthesis, adherence, acid/bile tolerance, antibiotic resistance. A scoring system used assess genomic profiles. highest-scoring underwent further evaluation. Consequently, two crispatus LG55-27 gasseri TM13-16, displayed an outstanding ability produce D-lactate adhere human epithelial cells. They also showed higher antimicrobial activity against Gardnerella vaginalis, Escherichia coli, Candida albicans, Staphylococcus aureus, Pseudomonas aeruginosa compared reference strains. Their resilience acid bile environments highlights potential oral supplementation. Oral administration these were tested bacterial vaginosis (BV) rat model at various doses. Results indicated that combined 1 × 106 CFU/day significantly mitigated BV rats. This research offers dosage guideline vaginitis therapy, underscoring efficient screening process probiotics using genome sequencing, testing, vivo experimentation.

Language: Английский

Citations

4
2002–2022 Quinolone Resistance in Escherichia coli of Swine in Mainland China: A Meta-Analysis DOI Creative Commons

Xuelin Long,

Shujun Liu,

Runmin Kang

et al.

Veterinary Sciences, Journal Year: 2025, Volume and Issue: 12(4), P. 345 - 345

Published: April 9, 2025

Swine have emerged as significant reservoirs of antibiotic-resistant strains and genes. To establish a sound rationale for applying quinolone antibiotics in the swine industry, meta-analysis was performed on resistance rate swine-derived Escherichia coli to China from 2002 2022. We systematically searched through three databases, PubMed, Web Science, CNKI, included 53 eligible studies within specified timeframe. Resistance rates were calculated using random-effects model Freeman–Tukey double-arcsine transformation. The observed follows: 37% levofloxacin (95% CI: 27~47%), 43% ofloxacin 34~51%), 54% enrofloxacin 46~62%), 50% ciprofloxacin 42~58%). Further subgroup analyses illuminated that higher mainland China’s eastern regions than western regions. Additionally, there no decrease over time different periods. From 2022, various exhibited differing degrees generalized quinolones. This poses potential public health risk underscores fact control remains prolonged ongoing challenge.

Language: Английский

Citations

0
Response of Escherichia coli to Acid Stress: Mechanisms and Applications—A Narrative Review DOI Creative Commons

Z.W. Li,

Zhaosong Huang,

Pengfei Gu

et al.

Microorganisms, Journal Year: 2024, Volume and Issue: 12(9), P. 1774 - 1774

Published: Aug. 28, 2024

Change in pH growth conditions is the primary stress for most neutralophilic bacteria, including model microorganism

Language: Английский

Citations

2
Effects of fecal microbiota transplantation combined with selenium on intestinal microbiota in mice with colorectal cancer DOI

Yintong Su,

Xing‐Xing Fan,

Xiaohua Cai

et al.

Biochemical and Biophysical Research Communications, Journal Year: 2024, Volume and Issue: 733, P. 150580 - 150580

Published: Aug. 23, 2024

Language: Английский

Citations

1
2002-2022 Quinolone Resistance in Escherichia Coli from Swine in Mainland China: A Meta-Analysis DOI

Xuelin Long,

Yue Sun,

Mingyue Tian

et al.

Published: Jan. 1, 2024

Language: Английский

Citations

0
Effect of Lippia origanoides Essential Oil Extract on Salmonella serovar Enteritidis as a Natural Alternative to Antibiotics in the Poultry Industry DOI

Abdiel Atencio-Vega,

Dante Javier Bueno, Juan D. Latorre

et al.

Published: Jan. 1, 2024

Language: Английский

Citations

0
Genome sequencing of Escherichia coli phage UFJF_EcSW4 reveals a novel lytic Kayfunavirus species DOI
Pedro Marcus Pereira Vidigal, Humberto Moreira Húngaro

3 Biotech, Journal Year: 2024, Volume and Issue: 15(1)

Published: Dec. 15, 2024

Language: Английский

Citations

0