The culture and identification of human placenta-derived mesenchymal stem cells DOI
Ruo‐Lan Du, Ting‐Hua Wang

Deleted Journal, Journal Year: 2024, Volume and Issue: unknown, P. 1 - 6

Published: Nov. 20, 2024

Objective: This study aimed to establish a reliable protocol for the cultivation and characterization of human placenta-derived mesenchymal stem cells (hPMSCs) evaluate their growth dynamics immunophenotype. Methods: hPMSCs were thawed cultured under controlled conditions using specialized serum-free medium. Cell viability morphology assessed an inverted microscope, medium changes performed bi-daily. For cell identification, immunofluorescence staining was conducted with antibodies CD44, CD90, CD45, characterized based on surface marker expression. Results: Cultured exhibited fibroblast-like rapid proliferation, particularly after reaching seeding density 50%. Growth curves indicated peak proliferation between days 3 4. Immunofluorescence analysis confirmed that positive CD90 but negative aligning typical profiles. Conclusion: The established successfully cultivated hPMSCs, demonstrating specific markers. These findings support potential application in regenerative medicine therapeutic research.

Language: Английский

Super-Enhancers in Placental Development and Diseases DOI Creative Commons

Gracy X. Rosario,

Sam P. Brown, Subhradip Karmakar

et al.

Journal of Developmental Biology, Journal Year: 2025, Volume and Issue: 13(2), P. 11 - 11

Published: April 9, 2025

The proliferation of trophoblast stem (TS) cells and their differentiation into multiple lineages are pivotal for placental development functions. Various transcription factors (TFs), such as CDX2, EOMES, GATA3, TFAP2C, TEAD4, along with binding sites cis-regulatory elements, have been studied roles in cells. While previous studies primarily focused on individual enhancer regions differentiation, recent attention has shifted towards investigating the role super-enhancers (SEs) different cell lineages. SEs clusters regulatory elements enriched transcriptional regulators, forming complex gene networks via differential patterns synchronized stimulation target genes. Although exact remains unclear, they commonly found near master regulator genes specific types implicated regulation tissue-specific lineage determination. Additionally, play a crucial regulating cellular growth both normal disease pathologies. This review summarizes advances SEs' pathophysiology diseases, emphasizing potential identifying SE-driven placenta to provide valuable insights developing therapeutic strategies address dysfunctions.

Language: Английский

Citations

0
Cell therapy with placenta-derived mesenchymal stem cells for secondary progressive multiple sclerosis patients in a phase 1 clinical trial DOI Creative Commons

Ameneh Shokati,

Mohsen Nikbakht,

Mohammad Ali Sahraian

et al.

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: May 8, 2025

Language: Английский

Citations

0
The culture and identification of human placenta-derived mesenchymal stem cells DOI
Ruo‐Lan Du, Ting‐Hua Wang

Deleted Journal, Journal Year: 2024, Volume and Issue: unknown, P. 1 - 6

Published: Nov. 20, 2024

Objective: This study aimed to establish a reliable protocol for the cultivation and characterization of human placenta-derived mesenchymal stem cells (hPMSCs) evaluate their growth dynamics immunophenotype. Methods: hPMSCs were thawed cultured under controlled conditions using specialized serum-free medium. Cell viability morphology assessed an inverted microscope, medium changes performed bi-daily. For cell identification, immunofluorescence staining was conducted with antibodies CD44, CD90, CD45, characterized based on surface marker expression. Results: Cultured exhibited fibroblast-like rapid proliferation, particularly after reaching seeding density 50%. Growth curves indicated peak proliferation between days 3 4. Immunofluorescence analysis confirmed that positive CD90 but negative aligning typical profiles. Conclusion: The established successfully cultivated hPMSCs, demonstrating specific markers. These findings support potential application in regenerative medicine therapeutic research.

Language: Английский

Citations

0