Development of a rapid and simple method for isolating apoplastic extracellular vesicles in plants DOI Creative Commons
Kenneth Sörensen, Joo-Hee Lee,

Tae-Hwan Kim

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 5, 2024

Abstract Recently, attention on plant extracellular vesicles (EVs) has increased alongside growing demands for standardizing their extraction methods. In this study, we compared three methods isolating EVs from plants, including a novel Enzyme method (E method) designed rapid apoplastic EV extraction. The E involves macerozyme-mediated washing fluid (AWF) collection followed by isolation using polymers and size-fractioning membranes. Compared to the freeze-grinding (F syringe-infiltration-ultracentrifugation (SI method), yielded more sized between 50–150 nm showed comparable purity based marker protein analysis. Additionally, it facilitated higher yield shorter times, making suitable both adult leaf seedling samples. Assessment in mutant backgrounds (patl1, syp61, pen1) confirmed method's robustness across different genetic contexts. These findings highlight as an efficient reliable approach characterization.

Language: Английский

Plant-Derived Extracellular Vesicles: Natural Nanocarriers for Biotechnological Drugs DOI Open Access
Eleonora Calzoni, Agnese Bertoldi, Gaia Cusumano

et al.

Processes, Journal Year: 2024, Volume and Issue: 12(12), P. 2938 - 2938

Published: Dec. 23, 2024

Plant-derived extracellular vesicles (PDEVs) are lipid bilayer nanoparticles, naturally produced by plant cells, with sizes ranging from 50 to 500 nm. Recent studies have highlighted their great potential in the biotechnological and medical fields, due natural origin, high biocompatibility intrinsic therapeutic properties. PDEVs contain a complex biological cargo of proteins, lipids, nucleic acids secondary metabolites, including antioxidants anti-inflammatory molecules, making them ideal for biomedical applications such as drug delivery. These play key role intercellular communication gene regulation, proving be particularly promising personalized medicine. ability improve stability bioavailability, optimizing targeted release minimizing side effects. Despite some challenges, compositional variability need standardized protocols, at gunsight innovative research aimed improving loading capacity specificity. This review aims provide comprehensive overview PDEVs, exploring structure, isolation methods, functional characteristics, applications, highlighting advantages over synthetic nanoparticles animal-derived vesicles, leading an sustainable solution development new approaches.

Language: Английский

Citations

4
Proteomic insights into fruit–pathogen interactions: managing biotic stress in fruit DOI
A Putra, Muhammad Naveed Khan,

Nurhaida Kamaruddin

et al.

Plant Cell Reports, Journal Year: 2025, Volume and Issue: 44(3)

Published: Feb. 13, 2025

Language: Английский

Citations

0
The Impact of Genotype and Controlled Environment Cultivation Parameters on Tomato-Leaf-Derived Exosome-like Nanoparticle Yield and Properties DOI Creative Commons
Akvilė Viršilė, G. Samuolienė, Kristina Laužikė

et al.

Horticulturae, Journal Year: 2024, Volume and Issue: 10(5), P. 477 - 477

Published: May 7, 2024

Horticultural plant material offers several advantages for isolating exosomes and other natural plant-derived exosome-like nanoparticles (PDENs) due to the accessibility affordability of widespread applications. This study aims explore impacts tomato genotype (‘Admiro’, ‘Roma’, ‘Brooklyn’, ‘Marmande’ ‘Betalux’) main cultivation parameters in controlled environment agriculture on yield properties their PDENs pharmaceutical cosmeceutical The PDEN yield, size distribution, antioxidative young seedlings were evaluated. ‘Betalux’ was distinguished by a remarkably higher nanoparticle concentration uniform distribution selected further experiments. impact temperature (18, 22, 26 °C), nitrogen nutrition (0, 250, 500 mg L−1), lighting photosynthetic photon flux density (PPFD; 150, 450 µmol m−2 s−1) investigated. Optimal conditions consisting 22 °C, 250 L−1 nutrition, s−1 PPFD used as reference. temperature, intensity resulted highest most particle preparations keratinocyte metabolic activity. Deviation from optimal reduced biomass protein yield.

Language: Английский

Citations

1
Influence of ZnSO4 and Methyl Jasmonate on the Metabolites and Bioactivity Present in Lemon-Fruit Membrane Vesicles DOI Open Access

Maria Gomez-Molina,

Micaela Carvajal, Paula García-Ibáñez

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(23), P. 12917 - 12917

Published: Nov. 30, 2024

Membrane vesicles isolated from vegetable tissues have shown promise in encapsulation technologies used industries like food and cosmetics, offering innovative approaches to product development. However, their associated linked metabolites not been studied. Lemon vesicle research only adds value the lemon crop (

Language: Английский

Citations

1
Development of a rapid and simple method for isolating apoplastic extracellular vesicles in plants DOI Creative Commons
Kenneth Sörensen, Joo-Hee Lee,

Tae-Hwan Kim

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 5, 2024

Abstract Recently, attention on plant extracellular vesicles (EVs) has increased alongside growing demands for standardizing their extraction methods. In this study, we compared three methods isolating EVs from plants, including a novel Enzyme method (E method) designed rapid apoplastic EV extraction. The E involves macerozyme-mediated washing fluid (AWF) collection followed by isolation using polymers and size-fractioning membranes. Compared to the freeze-grinding (F syringe-infiltration-ultracentrifugation (SI method), yielded more sized between 50–150 nm showed comparable purity based marker protein analysis. Additionally, it facilitated higher yield shorter times, making suitable both adult leaf seedling samples. Assessment in mutant backgrounds (patl1, syp61, pen1) confirmed method's robustness across different genetic contexts. These findings highlight as an efficient reliable approach characterization.

Language: Английский

Citations

0