Hypoxic preconditioning modulates BDNF signaling to alleviate depression-like behaviors in mice and its whole transcriptome sequencing analysis DOI Creative Commons
Lizhu Chen, Xujie Wang, Xiaoe Jia

et al.

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: May 2, 2025

Depression, a neurological disorder triggered by stressful stimuli such as hypoxia, is associated with high morbidity and mortality. Hypoxic preconditioning (HPC) an endogenous mechanism that has been used in recent research to upregulate BDNF, marker of depression, elicit neuroprotective effects. However, the mechanisms which HPC protects against depression remain poorly understood. Therefore, this study aimed investigate effects on depressive behaviors via BDNF signaling. Initially, ICR mice were subjected HPC, followed establishment 24-hour restraint stress model mimic behaviors. Subsequent analysis focused changes behaviors, biochemical markers, levels its ability modulate synaptic structure neurogenesis. Furthermore, whole transcriptome sequencing was conducted. The results indicated relieved characteristic mice, regulated neurotransmitter levels, elevated antioxidant capacity, promoted signaling hippocampus. PSD-95 expression, number complexity neuronal dendritic spines, hippocampal neurogenesis increased HPC. Restraint 373 DElncRNAs, 166 DEcircRNAs, 29 DEmiRNAs 1235 DEmRNAs, also modulated ceRNA networks constructed basis these DERNAs. Functional enrichment revealed genes are related synapses, neurotrophin These suggested upregulated activated BDNF/PLCγ/CREB alleviate deficits promote neurogenesis, ultimately ameliorating mice. identification various mRNAs ncRNAs their constituent ceRNAs provides theoretical guidance for clinical treatment

Language: Английский

Hypoxic preconditioning modulates BDNF signaling to alleviate depression-like behaviors in mice and its whole transcriptome sequencing analysis DOI Creative Commons
Lizhu Chen, Xujie Wang, Xiaoe Jia

et al.

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: May 2, 2025

Depression, a neurological disorder triggered by stressful stimuli such as hypoxia, is associated with high morbidity and mortality. Hypoxic preconditioning (HPC) an endogenous mechanism that has been used in recent research to upregulate BDNF, marker of depression, elicit neuroprotective effects. However, the mechanisms which HPC protects against depression remain poorly understood. Therefore, this study aimed investigate effects on depressive behaviors via BDNF signaling. Initially, ICR mice were subjected HPC, followed establishment 24-hour restraint stress model mimic behaviors. Subsequent analysis focused changes behaviors, biochemical markers, levels its ability modulate synaptic structure neurogenesis. Furthermore, whole transcriptome sequencing was conducted. The results indicated relieved characteristic mice, regulated neurotransmitter levels, elevated antioxidant capacity, promoted signaling hippocampus. PSD-95 expression, number complexity neuronal dendritic spines, hippocampal neurogenesis increased HPC. Restraint 373 DElncRNAs, 166 DEcircRNAs, 29 DEmiRNAs 1235 DEmRNAs, also modulated ceRNA networks constructed basis these DERNAs. Functional enrichment revealed genes are related synapses, neurotrophin These suggested upregulated activated BDNF/PLCγ/CREB alleviate deficits promote neurogenesis, ultimately ameliorating mice. identification various mRNAs ncRNAs their constituent ceRNAs provides theoretical guidance for clinical treatment

Language: Английский

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