Elicitor-driven enhancement of phenolic compounds in geranium callus cultures: phytochemical profiling via LC-MS/MS and biological activities DOI Creative Commons
Amine Elbouzidi, Mohamed Taibi, Abdellah Baraich

et al.

Frontiers in Chemistry, Journal Year: 2025, Volume and Issue: 13

Published: March 7, 2025

This research explores the effects of chitosan (CHT) and salicylic acid (SA) as elicitors on production phenolic flavonoid compounds in Pelargonium graveolens Hort. Callus cultures solid media, aiming to enhance antioxidant, anti-tyrosinase, anti-elastase properties. Calli were treated with various concentrations CHT (25, 50, 75, 100 mg/mL) SA µM), their phytochemical profiles examined through LC-MS/MS analysis. The findings indicated that notably enhanced levels total content (TPC) (TFC). greatest increase TPC was seen 25 µM (SA 2 ), recording 336.80 ± 8.35 mg/100 g dry weight (DW), mg/mL (CHT 5 which showed 325.74 7.81 DW. Among individual phenolics, kaempferol a remarkable under (192.82 17.99 DW) compared control (103.68 5.00 DW), treatment (119.68 12.01 DW). Additionally, rutin accumulation peaked at 30.64 3.00 DW treatment. Antioxidant activities, measured by DPPH TAC assays, also enhanced, treatments showing significant improvement over control. -elicited exhibited superior anti-tyrosinase IC 50 values 51.43 1.31 μg/mL, 35.42 4.42 31.84 0.60 respectively. These suggest effectively boost bioactive compound P. calli, subsequently biological activity, highlighting potential developing natural skincare products antioxidant anti-aging benefits.

Language: Английский

Elicitor-driven enhancement of phenolic compounds in geranium callus cultures: phytochemical profiling via LC-MS/MS and biological activities DOI Creative Commons
Amine Elbouzidi, Mohamed Taibi, Abdellah Baraich

et al.

Frontiers in Chemistry, Journal Year: 2025, Volume and Issue: 13

Published: March 7, 2025

This research explores the effects of chitosan (CHT) and salicylic acid (SA) as elicitors on production phenolic flavonoid compounds in Pelargonium graveolens Hort. Callus cultures solid media, aiming to enhance antioxidant, anti-tyrosinase, anti-elastase properties. Calli were treated with various concentrations CHT (25, 50, 75, 100 mg/mL) SA µM), their phytochemical profiles examined through LC-MS/MS analysis. The findings indicated that notably enhanced levels total content (TPC) (TFC). greatest increase TPC was seen 25 µM (SA 2 ), recording 336.80 ± 8.35 mg/100 g dry weight (DW), mg/mL (CHT 5 which showed 325.74 7.81 DW. Among individual phenolics, kaempferol a remarkable under (192.82 17.99 DW) compared control (103.68 5.00 DW), treatment (119.68 12.01 DW). Additionally, rutin accumulation peaked at 30.64 3.00 DW treatment. Antioxidant activities, measured by DPPH TAC assays, also enhanced, treatments showing significant improvement over control. -elicited exhibited superior anti-tyrosinase IC 50 values 51.43 1.31 μg/mL, 35.42 4.42 31.84 0.60 respectively. These suggest effectively boost bioactive compound P. calli, subsequently biological activity, highlighting potential developing natural skincare products antioxidant anti-aging benefits.

Language: Английский

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