Ethidium Bromide Degradation by Cold Atmospheric Plasma in Water and the Assessment of Byproduct Toxicity for Environmental Protection DOI Creative Commons

Reema Reema,

Tejas Bedmutha,

Nishanta Kakati

et al.

ACS Omega, Journal Year: 2024, Volume and Issue: 9(49), P. 48044 - 48054

Published: Nov. 25, 2024

Ethidium bromide (Et-Br) is a widely used fluorescent dye in molecular biology and biotechnology laboratories for visualizing nucleic acids agarose gel electrophoresis. However, concerns have been raised about its environmental impact potential health risks due to persistence toxicity. The accumulation long-term effects on the environment necessitate removal of Et-Br from water. This study investigates novel cold plasma technology degradation Et-Br. Cold atmospheric (CAP) an environmentally friendly that does not produce secondary pollutants generates variety potent chemical reactive oxidants such as hydroxyl radicals (

Language: Английский

Non-thermal plasma modulated l-tyrosine self-assemblies: a potential avenue for fabrication of supramolecular self-assembled biomaterials DOI Creative Commons
Priya Bhatt,

Prajakta Sharad Garad,

V. V. S. Prasanna Kumari Rayala

et al.

RSC Advances, Journal Year: 2024, Volume and Issue: 14(20), P. 13984 - 13996

Published: Jan. 1, 2024

Aromatic amino acids (AAs) have garnered particular interest due to their pivotal roles in numerous biological processes and disorders. Variations AA self-assembly not only affect protein structures functions, but non-covalent interactions such as hydrogen bonding, van der Waals forces, π-π stacking, yield versatile assemblies vital bio-inspired material fabrication. Tyrosine (Tyr), a non-essential aromatic acid, holds multifaceted significance the body building block, neurotransmitter precursor, thyroid hormone contributor, melanin synthesis regulator. The proficiency of Cold Atmospheric Plasma (CAP) generating spectrum reactive oxygen nitrogen species has spurred innovative research avenues studies biomolecular components, including its potential for targeted cancer cell ablation biomolecule modification. In this work, we assessed chemical well structural changes Tyrosine-derived self-assembled arising from CAP-induced species. For comprehensive understanding mechanism, different treatment times, feed gases, role solvent acidification are compared using various spectroscopic microscopic techniques. LC-ESI-QQQ mass spectra unveiled emergence oxygenated nitro derivatives l-tyrosine following interaction with CAP-derived ROS/RNS. SEM TEM images demonstrated an enhanced surface size formation novel nanomaterial-shaped CAP treatment. Overall, study aims explore CAP's single-amino hypothesizing creation supramolecular scrutinizing CAP-instigated transformations structures, potentially advancing biomimetic-attributed nanomaterial fabrication which might present frontier field designing functional biomaterials.

Language: Английский

Citations

5
Cold atmospheric pressure plasma assisted rapid assembly of peptide-based structures: a molecular scaffold to form supramolecular architectures DOI
Deepjyoti Basumatary,

Arya Gaikhe,

P. Radhakrishnanand

et al.

Journal of Physics D Applied Physics, Journal Year: 2024, Volume and Issue: 57(28), P. 285402 - 285402

Published: April 8, 2024

Abstract The use of cold atmospheric pressure plasma (CAPP) technology in the production peptide-based materials has shown great potential modern technology. Herein, two aggregation-prone oligopeptides, GNNQQNY and KLVFFA, were subjected to CAPP treatment form supramolecular assemblies/aggregates. Through peptide engineering biophysical techniques, effect CAPP-generated reactive oxygen nitrogen species on oligopeptides investigated for different times revealing that formation these aggregates primarily driven by electrostatic interactions without any chemical modifications. Field emission-scanning electron microscopy Thioflavin T (ThT) binding assay confirmed presence distinct β -strands, particularly KLVFFA upon irradiation. combination with self-assembly process characterization techniques employed this study holds promise development such structures based specific amino acid sequences.

Language: Английский

Citations

1
Green pathway of Urea Synthesis through Plasma-Ice Interaction: Optimization and Mechanistic Insights with N2 + CO2 and NH3 + CO2 Gas Mixtures DOI Creative Commons
Vikas Rathore

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: June 24, 2024

Abstract This study explores a green pathway for urea synthesis using plasma-ice interaction with gas mixtures of N2 + CO2 and NH3 CO2. Electrical optical emission spectroscopy were employed to characterize the plasmas, revealing that formation involves complex reactions driven by high-energy species, producing reactive nitrogen carbon intermediates further react form urea. Physicochemical analyses plasma-treated ice showed increased pH, electrical conductivity (EC), reduced oxidation-reduction potential (ORP). Optimization plasma process parameters (gas pressure, applied voltage, treatment time) was performed enhance formation. Among these parameters, time had most substantial influence. Increasing from 20 60 minutes significantly impacted physicochemical properties: plasma, pH 21.05%, EC 184.7%, ORP decreased 27.48%; 27.37%, 239.05%, 72.67%, respectively. The shows produces higher concentration (7.7 mg L-1) compared (0.55 L-1). is attributed direct availability reactivity ammonia, which simplifies reaction pathways enhances intermediate These findings highlight as an energy-efficient environmentally friendly method synthesis, offering sustainable alternative conventional processes.

Language: Английский

Citations

1
Green Pathway of Urea Synthesis Through Plasma–Ice Interaction: Optimization and Mechanistic Insights With N2 + CO2 and NH3 + CO2 Gas Mixtures DOI
Vikas Rathore, Vyom Desai,

N.I. Jamnapara

et al.

Plasma Processes and Polymers, Journal Year: 2024, Volume and Issue: 22(1)

Published: Oct. 28, 2024

ABSTRACT This study presents a green approach for urea synthesis using plasma–ice interaction with N₂ + CO₂ and NH₃ gas mixtures. Plasma characterization via electrical optical emission spectroscopy revealed that formation involves complex reactions driven by high‐energy species, producing reactive nitrogen carbon intermediates. Plasma‐treated ice exhibited increased pH, conductivity (EC), reduced oxidation–reduction potential (ORP). Optimization of parameters, especially treatment time, significantly influenced formation. The plasma produced higher concentration (7.7 mg L −1 ) compared to the (0.55 due ammonia's greater reactivity. method offers sustainable, energy‐efficient alternative traditional processes.

Language: Английский

Citations

1
Ethidium Bromide Degradation by Cold Atmospheric Plasma in Water and the Assessment of Byproduct Toxicity for Environmental Protection DOI Creative Commons

Reema Reema,

Tejas Bedmutha,

Nishanta Kakati

et al.

ACS Omega, Journal Year: 2024, Volume and Issue: 9(49), P. 48044 - 48054

Published: Nov. 25, 2024

Ethidium bromide (Et-Br) is a widely used fluorescent dye in molecular biology and biotechnology laboratories for visualizing nucleic acids agarose gel electrophoresis. However, concerns have been raised about its environmental impact potential health risks due to persistence toxicity. The accumulation long-term effects on the environment necessitate removal of Et-Br from water. This study investigates novel cold plasma technology degradation Et-Br. Cold atmospheric (CAP) an environmentally friendly that does not produce secondary pollutants generates variety potent chemical reactive oxidants such as hydroxyl radicals (

Language: Английский

Citations

0