Biological activity potential of Thalictrum orientale: antioxidant, anticholinesterase, and antiproliferative effects DOI Open Access
Oğuzhan Koçer

Artvin Çoruh Üniversitesi Orman Fakültesi Dergisi, Journal Year: 2025, Volume and Issue: 26(1), P. 222 - 229

Published: May 15, 2025

Plants are among the important natural resources in terms of biological activity. In this study, activity Thalictrum orientale species was evaluated comprehensively. For purpose, ethanol extract plant obtained by Soxhlet extraction method. To determine antioxidant capacity, total status (TAS) and oxidant (TOS) were analyzed using Rel Assay kits. Anticholinesterase determined measuring acetylcholinesterase (AChE) butyrylcholinesterase (BChE) enzyme activities. addition, antiproliferative on A549 lung cancer cell line. As a result analyzes, TAS value as 5.413±0.149 mmol/L, TOS 8.300±0.056 µmol/L oxidative stress index (OSI) 0.154±0.004. Within scope anticholinesterase activity, AChE inhibition 35.41±1.88 µg/mL BChE 58.63±1.24 µg/mL. It observed that effect increased direct proportion to increase concentration. The findings show has high potential antioxidant,

Language: Английский

Extract optimization of Ulva lactuca L. and biological activities of optimized extracts DOI Creative Commons
Nuh Korkmaz

BMC Biotechnology, Journal Year: 2025, Volume and Issue: 25(1)

Published: March 4, 2025

Algae are microscopic or macroscopic organisms that can photosynthesize and found in both freshwater saltwater ecosystems considered one of the cornerstones life. In our study, biological activities extracts produced under extract conditions provided highest activity Ulva lactuca L. were determined. Two different methods, Response Surface Method (RSM) Artificial Neural Network-Genetic Algorithm (ANN-GA) integration used for optimization. this context, optimum determined as 54.940 ˚C temperature, 6.513 h, 42.109 ethanol/water ratio according to RSM method 56.286 7.2793 36.8625 ANN-GA. The antioxidant optimized evaluated by Rel Assay kits, DPPH FRAP methods. Anticholinesterase testing against acetylcholinesterase butyrylcholinesterase enzymes. addition, antiproliferative effects examined A549 lung cancer cell line phenolic compound contents analyzed LC-MS/MS. As a result analyzes, it was seen obtained from recommended ANN-GA exhibited higher activities. Optimized U. have potential terms total value 6.272 ± 0.024 mmol/L. showed strong cytotoxic line. gallic acid, 4-hydroxybenzoic caffeic vanillic syringic quercetin kaempferol algae conditions. detected acid. addition all these properties, has anticholinesterase study. conclusion, attract attention with their well be source treatment management neurological diseases.

Language: Английский

Citations

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Bioactivity of Juglans regia kernel extracts optimized using response surface method and artificial neural Network-Genetic algorithm integration DOI Creative Commons
Ayşenur Gürgen

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: March 15, 2025

Abstract In this study, the biological activities of extracts obtained under optimum extraction conditions kernel part Juglans regia L. were determined. Two different methods, Response Surface Method (RSM) and Artificial Neural Network-Genetic Algorithm (ANN-GA) integration, used for optimization. The antioxidant capacity extract suggested by two methods was evaluated Rel Assay kits, DPPH FRAP methods. Anticholinesterase optimized measured action acetylcholinesterase butyrylcholinesterase enzymes. Antiproliferative effects tested on A549 lung cancer cell line. Phenolic compounds analyzed LC-MS/MS. It determined that both exhibited strong against line depending concentration increase. addition, it acetyl inhibition activity close to galantamine as a standard. extracts, 13 including gallic acid, catechinhyrate, 4-hydroxybenzoic caffeic vanillic syringic 2-hydoxycinamic resveratrol, myricetin, quercetin, kaempferol, protocatechuic acid 2-hyroxy1,4 naphthaquinone identified. predicted ANN-GA higher in general.

Language: Английский

Citations

0
Determination of optimum extract conditions and evaluation of biological activity potential of Salvia cilicica Boiss DOI Creative Commons
Oğuzhan Koçer

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: March 18, 2025

Plants are important natural resources used for many purposes. They especially stand out with their medical potential. In this study, the extract conditions showing highest biological activity were optimized using artificial intelligence application of Salvia cilicica Boiss. Then, activities determined. Response Surface Method was optimization. Using Box-Behnken experimental design, antioxidant capacities extracts evaluated through Rel Assay TAS and TOS kits, as well DPPH FRAP methods. Anticholinesterase assessed by measuring acetyl- butyrylcholinesterase enzyme activities. Total phenolic content quantified Folin-Ciocalteu reagent, while total flavonoid analyzed via aluminum chloride assay. The antiproliferative potential examined against A549 lung cancer cell line, compounds identified an LC-MS/MS system. Optimization results indicated that ideal extraction parameters 45.890 °C, 7.730 h, a 96.431 ethanol-to-water ratio. obtained under these exhibited value 7.461 ± 0.065 mmol/L, 5.630 0.163 µmol/L, OSI 0.075 0.002. contents measured 97.681 1.076 mg/g 113.067 0.621 mg/g, respectively. reached 70.669 0.199 determined 53.208 0.427 mg/g. Furthermore, anti-AChE anti-BChE recorded at 12.93 0.72 µg/mL 25.87 1.44 µg/mL, demonstrated notable cytotoxicity line. Additionally, 10 distinct in plant. These findings highlight significant S. optimal conditions, suggesting its valuable resource pharmacological applications.

Language: Английский

Citations

0
Optimization of Phellinus hartigii extracts: Biological activities, and phenolic content analysis DOI Creative Commons
Orhan Ünal, Ayşenur Gürgen, Tetiana Krupodorova

et al.

BMC Complementary Medicine and Therapies, Journal Year: 2025, Volume and Issue: 25(1)

Published: March 25, 2025

Medicinal mushrooms are sources of natural substances with diverse biological functions. The study evaluated the activity Phellinus hartigii (Allesch. & Schnabl) Pat. and optimized extraction conditions to maximize its bioactive potential. Extraction was performed using a Soxhlet apparatus under varying conditions: temperatures (30, 50, 70 °C), durations (1, 5.5, 10 h), ethanol/water ratios (0%, 50%, 100%). Total antioxidant status (TAS) analyzed across 17 experiments, optimal were identified response surface methodology (RSM). Extracts from further for capacity (Rel assay kits, DPPH, FRAP), anticholinesterase (acetyl- butyrylcholinesterase inhibition), antiproliferative (A549 lung cancer cell line), total phenolic content (Folin-Ciocalteu method), compound profile (LC–MS/MS). Optimal determined be 48.22 ˚C, 9.04 h, an ratio 52.22%. extract exhibited significant effects against A549 cells, increasing in concentration-dependent manner. inhibition values (IC50) acetylcholinesterase 21.29 ± 0.41 35.51 0.53 μg/mL, respectively. TPC (total content) value as 88.21 1.50 mg/g, FRAP 137.81 1.72 DPPH 106.07 2.44 TOS oxidant status) 9.27 0.06 µmol/L, TAS 4.98 0.03 mmol/L OSI (oxidative stress index) 0.19 0.002. LC–MS/MS analysis nine compounds, gallic acid catechin hydrate most abundant. P. obtained demonstrated substantial antioxidant, anticholinesterase, activities, highlighting therapeutic

Language: Английский

Citations

0
Extract optimization and biological activities of Otidea onotica using Artificial Neural Network-Genetic Algorithm and response surface methodology techniques DOI Creative Commons
Мustafa Sevindik, Celal Bal, Tetiana Krupodorova

et al.

BMC Biotechnology, Journal Year: 2025, Volume and Issue: 25(1)

Published: March 28, 2025

In this study, the biological activities of Otidea onotica were investigated using two optimization methods, Response Surface Methodology (RSM) and Artificial Neural Network-Genetic Algorithm (ANN-GA). The extracts tested for phenolic content, antioxidant potential, acetylcholinesterase butyrylcholinesterase inhibitory antiproliferative effects against A549 lung cancer cell line. results show that obtained by ANN-GA exhibited higher activity compared to RSM had total status (TAS), DPPH FRAP values. Phenolic content analysis revealed eight compounds with highest concentrations caffeic acid (in extract) gallic extract), respectively. Both showed strong cytotoxic cells depending on concentration, extract showing activity. Our study provides important findings therapeutic potential O. particularly reveals method plays an role in increasing can be used treatment neurodegenerative diseases future techniques offer effective strategy enriching contents.

Language: Английский

Citations

0
Biological activity potential of Thalictrum orientale: antioxidant, anticholinesterase, and antiproliferative effects DOI Open Access
Oğuzhan Koçer

Artvin Çoruh Üniversitesi Orman Fakültesi Dergisi, Journal Year: 2025, Volume and Issue: 26(1), P. 222 - 229

Published: May 15, 2025

Plants are among the important natural resources in terms of biological activity. In this study, activity Thalictrum orientale species was evaluated comprehensively. For purpose, ethanol extract plant obtained by Soxhlet extraction method. To determine antioxidant capacity, total status (TAS) and oxidant (TOS) were analyzed using Rel Assay kits. Anticholinesterase determined measuring acetylcholinesterase (AChE) butyrylcholinesterase (BChE) enzyme activities. addition, antiproliferative on A549 lung cancer cell line. As a result analyzes, TAS value as 5.413±0.149 mmol/L, TOS 8.300±0.056 µmol/L oxidative stress index (OSI) 0.154±0.004. Within scope anticholinesterase activity, AChE inhibition 35.41±1.88 µg/mL BChE 58.63±1.24 µg/mL. It observed that effect increased direct proportion to increase concentration. The findings show has high potential antioxidant,

Language: Английский

Citations

0