Green Extraction of Phenolic Compounds from Artichoke By-Products: Pilot-Scale Comparison of Ultrasound, Microwave, and Combined Methods with Pectinase Pre-Treatment DOI Creative Commons
Lidia Gil-Martínez, J.M. De La Torre, Sofía Martínez-López

et al.

Antioxidants, Journal Year: 2025, Volume and Issue: 14(4), P. 423 - 423

Published: March 31, 2025

The revalorization of artichoke (Cynara scolymus L.) by-products is a promising strategy to obtain bioactive compounds with antioxidant properties, supporting circular economy approach. This study compares the efficiency an enzymatic pretreatment followed by microwave-assisted extraction (EMAE), ultrasound-assisted (EUAE), and ultrasound-microwave-assisted (EUMAE) at pilot scale for recovering compounds. Extracts were purified using Diaion® HP20 resin phenolic-rich fractions enhanced activity. results showed that EUAE was most effective technique, achieving total phenolic content (TPC) 210.76 ± 1.40 µmol GAE/g d.w. yield 21.38%. HPLC-MS analysis identified 14 major compounds, including chlorogenic acid isomers (60.73 mg/g d.e.), caffeic (34.29 luteolin rutinoside (103.27 among others, which contribute extracts’ high bioactivity. potential extracts assessed Folin–Ciocalteu (F-C), ABTS, DPPH, FRAP assays. exhibited highest activity values, F-C: 985.33 4.46 d.e., ABTS: 80.46 2.39 TE/g DPPH: 87.03 1.11 FRAP: 184.99 2.52 d.e. purification process further TPC activity, enzyme–ultrasound-assisted extraction—purified extract (EUAE-PE) reaching purity 50.71% F-C value 2981.35 12.16

Language: Английский

Green Extraction and NMR Analysis of Bioactives from Orange Juice Waste DOI Creative Commons

Paula Scarabotto Penteado,

Maria Carolina Bezerra Di Medeiros Leal,

Maria G.A. Carosio

et al.

Foods, Journal Year: 2025, Volume and Issue: 14(4), P. 642 - 642

Published: Feb. 14, 2025

Brazil is a global leader in the orange industry, producing approximately one-fourth of world's oranges and generating over 50% associated waste. These by-products are rich bioactive compounds; however, their improper disposal poses environmental risks. This study employs an eco-friendly approach-microwave-assisted extraction-to recover valuable compounds from juice production The extracted were analyzed using nuclear magnetic resonance (NMR) gas chromatography-mass spectrometry (GC-MS). Key bioactives, including D-limonene, valencene, hesperidin, carbohydrates, successfully identified. NMR effectively traces semi-quantifies these compounds, while microwave-assisted extraction enables sustainable recovery high-purity confirmed by (87.66%) HPLC (84.30%) analyses.

Language: Английский

Citations

0
Microenvironment-Responsive Biomimetic Bioprosthetic Valve with Antithrombosis and Immunoregulation Performance DOI
Bo Wei, Lin Chen, Xueyu Huang

et al.

ACS Applied Materials & Interfaces, Journal Year: 2025, Volume and Issue: unknown

Published: March 14, 2025

The prevalence of heart valve disease (HVD) has escalated worldwide, because population aging. Currently, artificial replacement is considered the most effective treatment for HVD. complexity and risk have been markedly reduced with development minimally invasive interventional techniques, which resulted in more widespread implantation bioprosthetic valves (BHVs); however, they still present defects including thrombosis, poor cytocompatibility, immune responses, calcification, reduces their service life. We developed a microenvironment-responsive zwitterionic glycocalyx-mimetic hydrogel-engineered BHV (Hes@HS-PP) profile on-demand drug release. Inspired by structure function glycocalyx on inner wall blood vessels, hydrogel coating was covalently constructed photoinduced polymerization. This significantly resisted fouling components thrombosis improved endothelialization potential biocompatibility BHVs shielding interactions between xenogeneic collagenous matrix. Following introduction dynamic borate ester bonds into hydrogel, anti-inflammatory hesperidin (Hes) loaded onto BHVs. Excess reactive oxygen species were scavenged, Hes released inflammatory region demand to achieve regulation ameliorate reactions Moreover, Hes@HS-PP exhibited lower degree calcification rat subcutaneous model. In summary, construction hydrogels enhanced antithrombotic, anti-inflammatory, endothelialization, anticalcification properties mitigated structural valvular degradation, offering new perspectives functional modification

Language: Английский

Citations

0
Green Extraction of Phenolic Compounds from Artichoke By-Products: Pilot-Scale Comparison of Ultrasound, Microwave, and Combined Methods with Pectinase Pre-Treatment DOI Creative Commons
Lidia Gil-Martínez, J.M. De La Torre, Sofía Martínez-López

et al.

Antioxidants, Journal Year: 2025, Volume and Issue: 14(4), P. 423 - 423

Published: March 31, 2025

The revalorization of artichoke (Cynara scolymus L.) by-products is a promising strategy to obtain bioactive compounds with antioxidant properties, supporting circular economy approach. This study compares the efficiency an enzymatic pretreatment followed by microwave-assisted extraction (EMAE), ultrasound-assisted (EUAE), and ultrasound-microwave-assisted (EUMAE) at pilot scale for recovering compounds. Extracts were purified using Diaion® HP20 resin phenolic-rich fractions enhanced activity. results showed that EUAE was most effective technique, achieving total phenolic content (TPC) 210.76 ± 1.40 µmol GAE/g d.w. yield 21.38%. HPLC-MS analysis identified 14 major compounds, including chlorogenic acid isomers (60.73 mg/g d.e.), caffeic (34.29 luteolin rutinoside (103.27 among others, which contribute extracts’ high bioactivity. potential extracts assessed Folin–Ciocalteu (F-C), ABTS, DPPH, FRAP assays. exhibited highest activity values, F-C: 985.33 4.46 d.e., ABTS: 80.46 2.39 TE/g DPPH: 87.03 1.11 FRAP: 184.99 2.52 d.e. purification process further TPC activity, enzyme–ultrasound-assisted extraction—purified extract (EUAE-PE) reaching purity 50.71% F-C value 2981.35 12.16

Language: Английский

Citations

0