Transcriptomic Analysis Reveals That the Photosynthesis and Carotenoid Metabolism Pathway Is Involved in the Salinity Stress Response in Brassica rapa L. ssp. Pekinensis

Plants, Journal Year: 2025, Volume and Issue: 14(4), P. 566 - 566

Published: Feb. 13, 2025

Salinity stress is a major abiotic factor that adversely affects plant growth and development. This study investigated the physiological molecular responses of Brassica rapa L. ssp. Pekinensis to salinity by subjecting seedlings varying concentrations NaCl. Physiological analysis revealed significant wilting, chlorosis, marked reduction in chlorophyll carotenoid contents NaCl-treated seedlings, indicating impaired photosynthetic efficiency oxidative mitigation. RNA-seq identified extensive transcriptional reprogramming, with 6693 10,280 differentially expressed genes (DEGs) Z150 Z300 treatments, respectively, compared control group. DEGs were clustered into six expression trends, sustained up-regulation Clusters 2 6 down-regulation Cluster 3. Gene Ontology (GO) enrichment highlighted involvement these responses. Key encoding heat shock proteins, peroxidases, glutathione S-transferases, transcription factors significantly induced under stress, suggesting their roles adaptation. Furthermore, GO KEGG analyses associated photosynthesis carbon metabolism, disruption critical pathways. Weighted Co-expression Network Analysis (WGCNA) hub genes, such as histidine synthase low-density receptor-like protein, potentially central Additionally, metabolism was inhibited, key biosynthesis pathway. RT-qPCR validation confirmed reliability data. Collectively, findings provide comprehensive insights mechanisms underlying response B. highlighting potential targets for improving tolerance crops.

Language: Английский

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Isolating Water and Nitrogen Stress Tolerant Genotypes Among Tomato Landraces and Solanum pennellii Backcross Inbred Lines

Journal of Plant Growth Regulation, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 11, 2024

Improving crop adaptation to reduced agricultural inputs is a fundamental goal of current and future cropping systems, which must cope with resource scarcity feed an increasing world population. Landraces wild species showing tolerance traits are important source underexploited genetic that may contribute pursue this goal. In work, we exposed tomato lines different origin nitrogen water deficiency identify stress traits. Out 10 accessions, identified two genotypes, T292 T150, remarkable shortage, respectively. Under genotype did not show significant reduction the shoot biomass vs 20.2% observed in control M82. An increased antioxidant activity ability mobilize from roots were correlated sustained growth under low nitrogen. response drought stress, line T150 was best performer no compared control, well-watered conditions, whereas 27.6% deficiency, key adaptive included improve root:shoot ratio by allocating more roots, lower levels markers osmotic (proline LEA) plants. Overall, physiological/molecular mediate use confirmed landraces represent valuable be used modern sustainability-based breeding.

Language: Английский

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Effect of ex situ, eco-friendly ZnONPs incorporating green synthesised Moringa oleifera leaf extract in enhancing biochemical and molecular aspects of Vicia faba L. under salt stress

Green Processing and Synthesis, Journal Year: 2024, Volume and Issue: 13(1)

Published: Jan. 1, 2024

Abstract Nevertheless, the growing salt stress in arable areas poses a significant threat to agricultural production. The current study utilised zinc oxide nanoparticles (ZnONPs) using an extract obtained from leaves of Moringa oleifera (MOLe). purpose was reduce detrimental effect salinity on Vicia faba L . (VF) cultivar (Nubaria 3). biochemical and molecular characteristics plants grown saline settings were assessed 60 days after planting. expression levels plant defence genes polyphenol oxidase (PPO) peroxidase (POX) VF analysed at different time points. synthesised NPs improve osmotic protection under by elevated pigments involved photosynthesis. In addition, there increase parameters, such as total phenol, proline, antioxidant enzyme activities. use MOLe MOLe@ZnONPs resulted ion content considerable rise Vf POX PPO when exposed conditions. findings suggest that more efficient aiding recover negative impact stress.

Language: Английский

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Morpho-physiological and molecular responses of an ecologically important mangrove Kandelia candel (L.) Druce under salinity stress

Journal of Plant Biochemistry and Biotechnology, Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 30, 2024

Language: Английский

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