Prolactin drives cortical neuron maturation and dendritic development during murine embryonic stem cell differentiation

Frontiers in Cell and Developmental Biology, Journal Year: 2025, Volume and Issue: 13

Published: Feb. 26, 2025

Prolactin (PRL) is a pleiotropic hormone implicated in various physiological processes; however, its contribution to neurodevelopment, particularly early corticogenesis, remains insufficiently characterized. In this study, we investigate PRL's regulatory influence on the initial stages of cortical development, with an emphasis effects neuronal and astrocytic differentiation. We employed standardized vitro differentiation protocol generate neurons from mouse embryonic stem cells (mESCs). receptor (PRLr) expression was evaluated pluripotent cells, neural (NSCs), immature neurons, mature using both PCR immunofluorescence. These analyses revealed dynamic changes PRLr throughout process. Additionally, were treated varying concentrations PRL during late phases, enabling assessment impact phenotypic distribution morphological complexity. Early administration significantly enhanced population β-tubulin III + promoting survival without altering NSC proliferation. Furthermore, treatment increased abundance Tbr1 NeuN augmented dendritic complexity, accelerated maturation. contrast, exposure at later did not yield comparable effects. Notably, delayed maturation protoplasmic astrocytes, although total astrocyte affected. findings highlight pivotal role as regulator corticogenesis by modulating survival, thus emerges potential key factor underscoring importance hormonal regulation insights may have broader implications for understanding molecular cellular mechanisms underlying normal pathological neurodevelopment.

Language: Английский

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A role for JAK2 in mediating cell surface GHR-PRLR interaction

Published: March 17, 2025

Growth hormone (GH) receptor (GHR) and (full-length) prolactin (PRL) (PRLR) are transmembrane class I cytokine receptors that co-exist in various normal cancerous cells. Both respond to their associated ligands predominantly by activating the Janus Kinase 2 (JAK2)-signal transducer activator of transcription (STAT) signaling pathways, both also known initiate receptor-specific JAK2-independent signaling. Together with cognate ligands, these have been pro-tumorigenic effects cancers, including breast cancer (BC). Human GH is bind GHR PRLR, while PRL can only PRLR. A growing body work suggests PRLR form heteromers BC cells, modulating signal transduction. However, dynamics on plasma membrane how could affect respective still need be understood.To this end, we set out unravel spatiotemporal surface human T47D cells γ2A-JAK2 We applied direct stochastic optical reconstruction microscopy (dSTORM) quantified colocalization availability at nanometer scale different time points following treatment PRL. In co-expressing surprisingly observed not but induces a significant loss GHR. lacking or expressing mutant deficient JAK2 binding, downregulation cell membrane-bound GHR, no longer Colocalizations were confirmed proximity ligation (PL) assay.Our results suggest PRLR-GHR interaction, indirect, indispensable for PRL- GH- induced GH-induced PRL-induced increase potential consequences downstream Furthermore, our binding via intracellular domain’s Box1 element crucial regulation one receptor’s ligand-induced activation another receptor. Our findings shed new light reciprocal collective role play regulating

Language: Английский

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Sex-related gene expression in the posterodorsal medial amygdala of cycling female rats along with prolactin modulation of lordosis behavior

Brain Research, Journal Year: 2025, Volume and Issue: unknown, P. 149602 - 149602

Published: March 1, 2025

Language: Английский

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Dietary Angelica sinensis Enhances Sow Lactation and Piglet Development Through Gut Microbiota and Metabolism

Veterinary Sciences, Journal Year: 2025, Volume and Issue: 12(4), P. 370 - 370

Published: April 15, 2025

Piglets weaned per sow year (PSY) is a crucial metric for assessing the reproductive performances of sows and directly affects both productivity economic profitability pig farms [...]

Language: Английский

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