Ceapin-A7 suppresses the protective effects of Octreotide in human and bovine lung endothelial cells DOI Creative Commons

Saikat Fakir,

Madan Sigdel,

Md Matiur Rahman Sarker

et al.

Cell Stress and Chaperones, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 1, 2024

Endothelial injury can be the cause and consequence of severe inflammation injury. Synthetic somatostatin analogs - which suppress Growth Hormone are FDA-approved drugs associated with anti-inflammatory activities. In present study we suggest that protective activities Octreotide in human bovine endothelial cells mitigated by Ceapin-A7, is an activating transcription factor 6 suppressor. To function, assessed protein expression levels key cytoskeletal proteins, as well paracellular permeability. evaluate inflammation, measured factors promote vascular leak, reactive oxygen species generation. Collectively, our supports involvement effects barrier function.

Language: Английский

Update on regulation of GHRH and its actions on GH secretion in health and disease DOI
Antonio J. Montero‐Hidalgo, Mercedes del Río-Moreno, Jesús M. Pérez‐Gómez

et al.

Reviews in Endocrine and Metabolic Disorders, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 21, 2025

Language: Английский

Citations

1
The Potential Roles of 77C>T Variant and Growth Hormone-Releasing Hormone Elevation in Pituitary Adenoma Cases Associated with Acromegaly DOI Creative Commons
Thualfiqar Ghalib Turki,

Khadija abbas Sahan

Research Square (Research Square), Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 27, 2025

Abstract Background: Acromegaly (ACM) is an endocrine disorder characterized by the elevation of growth hormone-releasing hormone (GHRH), (GH), and insulin-like factor-1 (IGF-1). Objectives: This study aims to elucidate correlation between 77C>T (rs368475481) variant in GHRH gene elevated levels patients with acromegaly. Materials Methods: study, conducted from January August 2024, analyzed 80 samples Iraqi acromegaly collected at Diabetic National Center, Al-Mustansiriyah University, alongside healthy individuals. Growth were quantified using chemiluminescence immunoassay, while measured enzyme-linked immunosorbent assay (ELISA). The SNP was detected via Sanger sequencing assess genetic variation controls. Results: serum GHRH, GH, IGF-1 compared results indicate presence rs368475481 amplified fragment (a 791 bp amplicon including exons 2 3, intron 2), CC homozygous genotype observed (100%) controls 40 (50%) patients. CT heterozygous present Conclusion: The polymorphism shown be associated pituitary adenomas, which cause

Language: Английский

Citations

0
Mention to Prof. Andrew V. Schally DOI Creative Commons
Riccarda Granata

Reviews in Endocrine and Metabolic Disorders, Journal Year: 2025, Volume and Issue: unknown

Published: March 17, 2025

Language: Английский

Citations

0
Growth Hormone-Releasing Hormone Antagonists Increase Radiosensitivity in Non-Small Cell Lung Cancer Cells DOI Open Access
Iacopo Gesmundo, Francesca Pedrolli, F.R. Giglioli

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(7), P. 3267 - 3267

Published: April 1, 2025

Growth hormone-releasing hormone (GHRH) antagonists exert antitumor functions in different experimental cancers. However, their role combination with radiotherapy non-small cell lung cancer (NSCLC) remains unknown. Therefore, we investigated the radiosensitizing effect of GHRH NSCLC. A549 and H522 NSCLC lines were exposed to ionizing radiation (IR) MIA-602 MIA-690, either individually or combination. Cell viability proliferation evaluated by MTT, BrdU, flow cytofluorimetry, clonogenic assays; gene protein expression, signaling pathways, apoptosis analyzed real-time PCR, Western blot, annexin staining, caspase-3 assay. showed effects alone potentiated IR-induced inhibition proliferation. The MIA-690 IR decreased expression receptor, its oncogenic splice variant 1, IGF1 mRNA levels. Additionally, cycle inhibitors proapoptotic markers upregulated, whereas cyclins, MYC, antiapoptotic Bcl-2 downregulated. Radioresistance was prevented which also blunted epithelial-mesenchymal transition enhancing E-cadherin reducing mesenchymal, oxidative, proangiogenic effectors. Finally, both enhanced radiosensitivity primary human cells. These findings highlight potential as radiosensitizers treatment.

Language: Английский

Citations

0
Ectopic GHRH production: revisiting a rare cause of acromegaly DOI
Matheo Augusto Morandi Stumpf, Nathalie Oliveira de Santana, Márcio Carlos Machado

et al.

Reviews in Endocrine and Metabolic Disorders, Journal Year: 2025, Volume and Issue: unknown

Published: April 2, 2025

Language: Английский

Citations

0
Lanreotide protects against LPS-induced inflammation in endothelial cells and mouse lungs DOI
Md Matiur Rahman Sarker,

Saikat Fakir,

Khadeja-Tul Kubra

et al.

Tissue Barriers, Journal Year: 2025, Volume and Issue: unknown

Published: April 17, 2025

Somatostatin is expressed in various tissues - including the hypothalamus and strongly suppresses Growth Hormone levels to maintain homeostasis. Synthetic somatostatin analogs are currently used clinics treat neuroendocrine tumors acromegaly. An emerging body of evidence suggests that those synthetic peptides exert anti-inflammatory activities. The present study examines effect Lanreotide (LAN) on Lipopolysaccharide (LPS)-triggered injury endothelial cells mice. Our findings indicate LAN effectively mitigates LPS-induced hyperpermeability, inflammation, reactive oxygen species (ROS) generation bovine pulmonary artery (BPAEC) human lung microvascular (HULEC-5a). A murine model acute was also utilized, examine effects edema inflammation. observations suggest myosin light chain 2 (MLC2), Cofilin, extracellular signal-regulated kinase 1/2 (ERK1/2), STAT1, STAT3, P38 activation; edema. In conclusion, based aforementioned observations, it suggested counteracts experimental

Language: Английский

Citations

0
Ceapin-A7 suppresses the protective effects of Octreotide in human and bovine lung endothelial cells DOI Creative Commons

Saikat Fakir,

Madan Sigdel,

Md Matiur Rahman Sarker

et al.

Cell Stress and Chaperones, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 1, 2024

Endothelial injury can be the cause and consequence of severe inflammation injury. Synthetic somatostatin analogs - which suppress Growth Hormone are FDA-approved drugs associated with anti-inflammatory activities. In present study we suggest that protective activities Octreotide in human bovine endothelial cells mitigated by Ceapin-A7, is an activating transcription factor 6 suppressor. To function, assessed protein expression levels key cytoskeletal proteins, as well paracellular permeability. evaluate inflammation, measured factors promote vascular leak, reactive oxygen species generation. Collectively, our supports involvement effects barrier function.

Language: Английский

Citations

0