
bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown
Published: Dec. 16, 2024
Abstract The aim of this study was to explore the viability dual production polyhydroxybutyrate (PHB) and exopolysaccharides (EPS) by seven microbiomes rich in cyanobacteria. Our initial experiments involved screen for EPS-producing candidates examine impact salinity acetate on EPS synthesis. Salinity’s known influence acetate’s role enhancing PHB guided our parameter selection. Surprisingly, neither introduction an external carbon source (acetate) nor exposure abiotic stressor (salt) significantly altered synthesis rates, which ranged from 25 150 mg·L -1 , or its composition, with glucose being dominant sugar component. Scaling up a 3 L photobioreactor, we achieved simultaneous biopolymer production, reaching 205 87 PHB. Additionally, presence uronic acid facilitated biomass flocculation, streamlining separation process, potentially reducing associated time costs.
Language: Английский