In Vitro Initiation, Regeneration, and Characterization of Plants Derived from Mature Tetraploid Floral Explants of Date Palm (Phoenix dactylifera L.) DOI Creative Commons

Ahmed Othmani,

Amel Sellemi,

Monia Jemni

et al.

Horticulturae, Journal Year: 2024, Volume and Issue: 10(11), P. 1206 - 1206

Published: Nov. 15, 2024

A stable tetraploid date palm mutant with the potential for increased fruit size was recovered from a sectorial di-tetraploid chimera via in vitro culture of flower explants. Callus induced using 2,4-D, followed by shoot regeneration on medium containing NAA and BAP rooting IBA. Regenerated plantlets, confirmed as flow cytometry, were acclimatized grown six years. The leaves tetraploids exhibited significantly wider petiole bases, thicker rachis spines, broader leaflets, more intense green leaf color compared to diploids. However, length, spine count, overall number reduced. This is first report successful recovery chimeric palm, demonstrating this technique generate novel germplasm highlighting phenotypic differences associated tetraploidy species.

Language: Английский

Integrating In Vitro Cultivation and Sustainable Field Practices of Sacha Inchi (Plukenetia volubilis L.) for Enhanced Oil Yield and Quality: A Review DOI Creative Commons
Pramesti Istiandari, Ahmad Faizal

Horticulturae, Journal Year: 2025, Volume and Issue: 11(2), P. 194 - 194

Published: Feb. 12, 2025

Sacha inchi (Plukenetia volubilis), or the Inca peanut, is a promising functional food and sustainable alternative to traditional oilseed crops like soybean. Its seeds are rich in omega-3, omega-6, omega-9 fatty acids, high-quality protein, bioactive compounds, offering significant nutritional health benefits. Moreover, sacha cultivation thrives on degraded soils with minimal agrochemical input, supporting biodiversity reducing environmental impacts. Despite its potential, large-scale faces challenges such as genetic variability, low seed viability, susceptibility pests diseases, resulting inconsistent yields plant quality. In vitro propagation presents viable solution, enabling production of genetically uniform, disease-free seedlings under controlled conditions. Successful depends factors explant selection, growth regulator combinations, medium composition, control. Advances these techniques have improved outcomes other crops, enhanced germination, oil yield, stability, offer similar opportunities for inchi. By integrating field techniques, this review highlights potential nutritionally rich, agricultural solution. These findings provide foundation advancing cultivation, ensuring productivity, quality, greater accessibility benefits around world.

Language: Английский

Citations

0
Biochar: A Valid Additive to Enhance Kiwifruit In Vitro Proliferation DOI Creative Commons
Samreen Nazeer,

Valentina Morresi,

Francesca Balducci

et al.

Agronomy, Journal Year: 2025, Volume and Issue: 15(4), P. 849 - 849

Published: March 28, 2025

Biochar, a by-product of agri-food waste, has shown benefits in plant growth and soil health. However, its use vitro remains underexplored. This study investigates the impact biochar supplementation culture medium, alone or combination, with 6-benzylaminopurine (BAP), on kiwifruit (Actinidia chinensis var. deliciosa), cv. Tomuri proliferation. Kiwifruit explants were cultured media enriched 0, 4, 6 g/L biochar, without BAP (0.2 mg/L), over two subcultures (SUB1 SUB2). Parameters such as shoot root number length, fresh dry weight, well plantlets’ total phenolic content antioxidant activity, measured analyzed. Biochar enhanced plantlets proliferation, particularly BAP. In SUB1, at 4 g/L, promoted production (2.00 vs. 1.63) their length (1.50 cm 0.98), independently presence The BAP-free media, favored rhizogenesis; SUB2, where average, 5.58 roots per recorded. increased especially media. addition an additive to medium during proliferation phase could be breakthrough outcome for nursery sector.

Language: Английский

Citations

0
Plant growth regulator studies and emerging biotechnological approaches in Artemisia annua L.: A comprehensive overview DOI
Dexter Achu Mosoh

South African Journal of Botany, Journal Year: 2025, Volume and Issue: 181, P. 181 - 222

Published: April 21, 2025

Language: Английский

Citations

0
In Vitro Initiation, Regeneration, and Characterization of Plants Derived from Mature Tetraploid Floral Explants of Date Palm (Phoenix dactylifera L.) DOI Creative Commons

Ahmed Othmani,

Amel Sellemi,

Monia Jemni

et al.

Horticulturae, Journal Year: 2024, Volume and Issue: 10(11), P. 1206 - 1206

Published: Nov. 15, 2024

A stable tetraploid date palm mutant with the potential for increased fruit size was recovered from a sectorial di-tetraploid chimera via in vitro culture of flower explants. Callus induced using 2,4-D, followed by shoot regeneration on medium containing NAA and BAP rooting IBA. Regenerated plantlets, confirmed as flow cytometry, were acclimatized grown six years. The leaves tetraploids exhibited significantly wider petiole bases, thicker rachis spines, broader leaflets, more intense green leaf color compared to diploids. However, length, spine count, overall number reduced. This is first report successful recovery chimeric palm, demonstrating this technique generate novel germplasm highlighting phenotypic differences associated tetraploidy species.

Language: Английский

Citations

0