Immune response and protection efficacy of formalin‐killed vaccines against Streptococcus iniae in four‐finger threadfin Eleutheronema tetradactylum DOI Open Access
Yin‐Ze Shi, Andre Giovanni, Li‐Wu Cheng

et al.

Journal of Fish Diseases, Journal Year: 2024, Volume and Issue: 47(11)

Published: Aug. 29, 2024

Abstract Four‐finger threadfin, Eleutheronema tetradactylum farming in southern Taiwan has been facing disease problems caused by Streptococcus iniae since 2018. The development of a vaccine against infectious S . the cultured threadfin industry is necessary. Thus, this study aimed to examine efficacy immunized formalin‐killed cells (FKC) from GSI‐111 for 42 days post‐vaccination (dpv) using two doses FKC alone (a booster at 14 dpv) as group A, and mixed with ISA763A adjuvant single dose B or double C. Immunoglobulin (Ig)‐M was purified rabbit anti‐threadfin IgM polyclonal antibodies were used detect antibody level fish; vaccinated A displayed higher levels 3 dpv all treatments demonstrated high between dpv. All groups showed significantly values lysozyme activity compared control group; peaked expression profiles pro‐inflammatory immune‐related genes, TNF‐α , IL‐12A C2 upregulated dpv, while CD8A chemokine receptor CXCR4 Finally, threadfins challenged average relative percent survival 96% vaccination treatments, 100% C treatment. In summary, that vaccines whether formulated an could stimulate immune response effective protect infection.

Language: Английский

Investigating the effect of hydroalcoholic extract of red algae (Laurencia caspica) on growth performance, mucosal immunity, digestive enzyme activity and resistance to Streptococcus iniae and Aeromonas hydrophila in Nile tilapia (Oreochromis niloticus) DOI Creative Commons
Majid Khanzadeh, Seyed Hossein Hoseinifar, Babak Beikzadeh

et al.

Aquaculture Reports, Journal Year: 2024, Volume and Issue: 35, P. 101984 - 101984

Published: Feb. 22, 2024

Immunostimulants, including seaweed, can play a significant role in increasing the immune response and preventing occurrence of diseases aquatic animals. These stimulants increase fish's disease resistance by strengthening nonspecific system. This study aimed to investigate effects red macroalgae extract Laurencia caspica (Lc) on mucosal immunity, growth, serum biochemical parameters, mucus bactericidal activity, digestive enzymes, against S. iniae A. hydrophila Nile tilapia. Fish with an average weight 100 ± 2 g were fed Lc algae at four levels (0, 0.5, 1 2%) for 50 days. The gain (WG), food conversion ratio (FCR), specific growth rate (SGR) significantly increased compared control group (P < 0.05). activity was no effect Streptococcus (S. iniae), > However, it Additionally, there Aeromonas (A. hydrophila) not amount total protein (Tp), albumin (Alb), globulin (Glb), cholesterol (Chol) 0.05), although triglyceride (TG) 2% 25th day had Lysozyme (except 0.5% 50th day) immunoglobulin (Ig) (in all groups) enzymes such as trypsin α-amylase days Lipase enzyme extracts groups substantially survival fish challenged higher than 0.0001). Overall, be concluded that immunity. Also, induces improve tilapia growth.

Language: Английский

Citations

9

Effects of Dietary Supplements Sargassum ilicifolium and Spirulina platensis on Growth Parameters, Immunity and Gene Expression in Juvenile Asian Seabass (Lates calcarifer) DOI Creative Commons
Majid Khanzadeh, Seyed Hossein Hoseinifar,

Ashkan Zargari

et al.

Journal of Agriculture and Food Research, Journal Year: 2025, Volume and Issue: 19, P. 101689 - 101689

Published: Jan. 30, 2025

Language: Английский

Citations

1

The Effects of Laurencia caspica Algae Extract on Hemato-Immunological Parameters, Antioxidant Defense, and Resistance against Streptococcus agalactiae in Nile tilapia (Oreochromis niloticus) DOI Creative Commons
Majid Khanzadeh, Babak Beikzadeh, Seyed Hossein Hoseinifar

et al.

Aquaculture Nutrition, Journal Year: 2023, Volume and Issue: 2023, P. 1 - 13

Published: July 4, 2023

Natural immune stimulants are among the most effective chemicals for boosting immunity and fish welfare. This study aims to investigate effects of red macroalgae extract (Laurencia caspica) on hematological, immunological, antioxidant, biochemical, disease resistance against S. agalactiae in Nile tilapia 50 days. For this purpose, fishes were assigned four dietary treatments group which base meal was supplemented with 0.5%, 1%, 2% L. caspica extract. On days 25 experiment, samples taken antioxidant parameters. The white blood cells (WBCs), hemoglobin, neutrophils significantly increased after feeding extract, but until 25th day, no significant difference observed except hemoglobin. Immunological parameters (including Immunoglobulin M [IgM] complement 3 [C3]) higher treated groups compared control both posttreatment. However, noticed between case lysozyme activity. Alkaline phosphatase (ALP) alanine aminotransferase (ALT) considerably comparison 50th day. In addition, enzyme activities day (L. 1% peroxidase [POD] superoxide dismutase [SOD] all groups) (catalase [CAT], SOD POD) spleen. survival rate challenged Streptococcus greater than group. Finally, it can be concluded that is an immunological stimulant induces agalactiae.

Language: Английский

Citations

18

Fucoidan derived from Sargassum ilicifolium affects growth and hemato-immunological parameters and antioxidant defense in Oscar (Astronotus ocellatus) DOI Creative Commons
Majid Khanzadeh, Seyed Hossein Hoseinifar,

Ashkan Zargari

et al.

Frontiers in Marine Science, Journal Year: 2024, Volume and Issue: 11

Published: April 24, 2024

Fucoidan (Fuc) is a sulfated polysaccharide derived from brown algae and has various biological activities such as immune modulator, growth enhancer, antioxidant bactericidal. However, there no information about the effect of fucoidan on ornamental fish. This study investigated Sargassum ilicifolium parameters, immunity, biochemical, antioxidant, bactericidal activity serum in Oscar. Fish weighing 49.75 ± 0.96 g were fed S. at four levels (0%, 0.5%, 1%, 2%) for 50 days. After days feeding with fucoidan, was significant increase blood parameters except WBCs (Fuc compared to control group ( p &lt; 0.05). The weight gain (WG), feed conversion ratio (FCR), specific rate (SGR) after had Serum biochemical total protein (Tp) globulin (Glb) groups 0.05), but difference observed albumin (Alb) &gt; Antioxidant serum, SOD CAT, In addition, lysozyme complement containing significantly increased A NBT against Aeromonas hydrophila Fuc 2% skin carotenoids 1% Overall, it can be concluded that parameters. could pigmentation, enzyme activity, A. .

Language: Английский

Citations

7

Immuno-protective response of Asian seabass (Lates calcarifer) to inactivated vaccines against Streptococcus iniae and Vibrio harveyi DOI Creative Commons
Ahmad Erfanmanesh, Babak Beikzadeh, Majid Khanzadeh

et al.

BMC Veterinary Research, Journal Year: 2024, Volume and Issue: 20(1)

Published: March 8, 2024

Abstract Background In this study, the protective immunity and immunogenicity of monovalent bivalent Streptococcus iniae Vibrio harveyi vaccine were evaluated in Asian seabass. To analyze immune responses, 1200 seabass with an average weight 132.6 ± 25.4 g divided into eight treatments triplicates (50 fish per tank) as follows: S. immunized by injection (SI), V. (VI), (SVI) injection, immersion (SIM), (VIM) immersion, harvei (SVIM) phosphate-buffered saline (PBS) (PBSI) control group without administration (CTRL). Blood serum samples taken at end 30th 60th days. Then vaccinated groups challenged two bacteria ( ) separately mortality was recorded for 14 Results This study reveals that there is no significant difference hematological parameters on days experiment vaccine-immunized compared to CTRL P > 0.05). Meanwhile, amount albumin level, respiratory burst activity, bactericidal activity Total protein day (in VI SVI groups), globulin groups) group) PBSI had a increase < Complement lysozyme SI increased significantly Serum antibody titer against SI, VI, SVIM A relative percentage survival (RPS) following challenge (86.6%), (83.3%,) (73.3%) observed (43.3%) (40%) Also, RPS after group, 86.6%, 83.3%, VIM 80% 76.6% (46.6%) (50%) Conclusion Overall, results demonstrated harvey able produce

Language: Английский

Citations

5

Effects of artichoke (Cynara scolymus) polyphenolic extract on growth, antioxidants and some immune parameters in common carp (Cyprinus carpio) DOI Creative Commons
Eshrat Beigom Kia, Seyed Hossein Hoseinifar, Mohammad Mazandarani

et al.

BMC Veterinary Research, Journal Year: 2025, Volume and Issue: 21(1)

Published: March 17, 2025

In this research, Cyprinus carpio juveniles were fed with experimental diets containing different levels of artichoke polyphenolic leaf extract (ALE) 0 (Control), 0.5, 1, and 2 g/kg over eight weeks. After weeks, haemato-immunological parameters, growth performance, immune parameters antioxidant defense measured. The administration varying ALE had no significant effects on compared to the control group. However, haematological significantly elevated Immune such as serum lysozyme, mucus total immunoglobulin, well alternative complement activity bactericidal ALE-treated carps, showed a notable increase in comparison CTRL Also, agglutination titer carps higher than Feeding (0.5, g/kg) enhanced activities enzymes, including superoxide dismutase all groups, catalase 0.5 glutathione peroxidase 1 results study demonstrate that dietary supplementation at 0.5–2 positively influences factors carpio, most pronounced observed g/kg. These findings suggest extract, particularly dosages, has potential an effective feed additive enhance immunity capacity carp farming.

Language: Английский

Citations

0

Current knowledge of lactococcosis in rainbow trout: Pathogenesis, immune response and prevention tools DOI Creative Commons
Sarker Mohammed Ibrahim Khalil, Massimo Orioles, Paolo Tomè

et al.

Aquaculture, Journal Year: 2023, Volume and Issue: 580, P. 740363 - 740363

Published: Nov. 15, 2023

Lactococcosis caused by Lactococcus garvieae has emerged as one of the most devastating bacterial disease affecting rainbow trout (Oncorhynchus mykiss) worldwide. Understanding immune response to this streptococcal pathogen is crucial for diagnosing and managing associated developing vaccines preventive measures, such selection naturally resistant stocks. Over past few decades, a significant number studies have focused on several aspects lactococcosis, including pathological features, responses vaccination protocols. However, disease's geographical distribution its spread over time, routes entry dissemination within host's body, progression infection, subsequent inflammatory host still need be fully explored. This review describes extant knowledge pathogenesis, prevention tools lactococcosis identify areas that require further investigations dedicated studies. Particular emphasis been placed describe current understanding how parameters mechanisms are affected in when they exposed natural or experimental infections undergo treatments. Ultimately, more comprehensive interaction between L. will contribute effective strategies aquaculture settings.

Language: Английский

Citations

7

Immune response and protection efficacy of formalin‐killed vaccines against Streptococcus iniae in four‐finger threadfin Eleutheronema tetradactylum DOI Open Access
Yin‐Ze Shi, Andre Giovanni, Li‐Wu Cheng

et al.

Journal of Fish Diseases, Journal Year: 2024, Volume and Issue: 47(11)

Published: Aug. 29, 2024

Abstract Four‐finger threadfin, Eleutheronema tetradactylum farming in southern Taiwan has been facing disease problems caused by Streptococcus iniae since 2018. The development of a vaccine against infectious S . the cultured threadfin industry is necessary. Thus, this study aimed to examine efficacy immunized formalin‐killed cells (FKC) from GSI‐111 for 42 days post‐vaccination (dpv) using two doses FKC alone (a booster at 14 dpv) as group A, and mixed with ISA763A adjuvant single dose B or double C. Immunoglobulin (Ig)‐M was purified rabbit anti‐threadfin IgM polyclonal antibodies were used detect antibody level fish; vaccinated A displayed higher levels 3 dpv all treatments demonstrated high between dpv. All groups showed significantly values lysozyme activity compared control group; peaked expression profiles pro‐inflammatory immune‐related genes, TNF‐α , IL‐12A C2 upregulated dpv, while CD8A chemokine receptor CXCR4 Finally, threadfins challenged average relative percent survival 96% vaccination treatments, 100% C treatment. In summary, that vaccines whether formulated an could stimulate immune response effective protect infection.

Language: Английский

Citations

0