Antibiotics in semen extenders – a multiplicity of paradoxes
Reproduction Fertility and Development,
Journal Year:
2024,
Volume and Issue:
36(5)
Published: March 6, 2024
Addition
of
antibiotics
to
semen
extenders
was
taken
for
granted
many
years,
from
the
time
that
commercial
artificial
insemination
in
livestock
first
began
decades
ago.
However,
there
is
now
a
growing
realisation
this
non-therapeutic
utilisation
antibacterial
agents
contrary
current
recommendations
prudent
use
medical
and
veterinary
professionals
are
advised
follow.
Furthermore,
not
benign,
having
negative
effects
on
sperm
samples,
inseminated
female,
personnel
potentially
environment.
The
purpose
review
three-fold:
highlight
fact
used
extenders,
with
result
considerable
amounts
globally
animal
breeding,
recent
studies
aspects
using
purpose,
look
at
possible
alternatives.
Recent
changes
legislation
regarding
occurred
some,
but
all,
countries,
leaving
question
marks
producers
as
whether
should
be
added
or
not.
Language: Английский
Bacterial diversity in semen from stallions in three European countries evaluated by 16S sequencing
Veterinary Research Communications,
Journal Year:
2024,
Volume and Issue:
48(3), P. 1409 - 1421
Published: Feb. 2, 2024
Abstract
The
microbiome
plays
a
significant
role
in
shaping
the
health
and
functioning
of
systems
it
inhabits.
seminal
stallions
has
implications
for
reproductive
tract,
sperm
quality
during
preservation
antibiotic
use
semen
extenders.
Diverse
bacteria
are
present
on
external
genital
tract
mix
commensal
microorganisms
populates
various
parts
influencing
bacterial
content.
Other
sources
include
environment,
collection
equipment,
personnel.
load
can
adversely
affect
fertility,
particularly
artificial
insemination,
where
is
extended
stored
before
use.
Antibiotics
frequently
used
to
inhibit
growth,
but
their
effectiveness
varies
depending
strains
present.
aim
this
study
was
assess
diversity
from
37
healthy
across
three
European
nations
(Germany,
Portugal,
Sweden)
using
16S
sequencing.
Semen
samples
were
collected
individual
at
AI
centers;
DNA
extraction,
sequencing,
bioinformatic
analysis
performed.
Differences
among
seen;
although
phyla
shared
regions,
differences
observed
genus
level.
Climate,
husbandry
practices,
variability
likely
contribute
these
differences.
These
findings
underscore
importance
tailoring
strategies
based
regional
profiles.
presents
comprehensive
approach
understanding
intricacies
stallion
its
potential
technologies
animal
health.
Language: Английский
Recent advancements to increase success in assisted reproductive technologies in cattle
Animal Reproduction,
Journal Year:
2024,
Volume and Issue:
21(3)
Published: Jan. 1, 2024
Assisted
reproductive
technologies
(ART)
are
fundamental
for
cattle
breeding
and
sustainable
food
production.
Together
with
genomic
selection,
these
contribute
to
reducing
the
generation
interval
accelerating
genetic
progress.
In
this
paper,
we
discuss
advancements
in
used
fertility
evaluation
of
animals,
collection,
processing,
preservation
gametes.
It
is
utmost
importance
industry
select
dams
sires
next
as
young
possible,
efficient
timely
collection
There
a
need
reliable
easily
applicable
methods
evaluate
sexual
maturity
fertility.
Although
gametes
processing
have
been
improved
recent
decades,
challenges
still
encountered.
The
targeted
use
sexed
semen
beef
has
obliterated
production
surplus
replacement
heifers
bull
calves
from
dairy
breeds,
markedly
improving
animal
welfare
ethical
considerations
practices.
Parallel
new
technologies,
many
well-established
remain
relevant,
although
evolving
applications.
Language: Английский
Bacterial survival below zero: Impact of storage time on bacterial viability in bull semen
Cryobiology,
Journal Year:
2025,
Volume and Issue:
119, P. 105233 - 105233
Published: March 19, 2025
Although
freezing
methods
have
been
optimized
for
preserving
sperm
integrity,
their
effectiveness
in
sustaining
bacterial
viability
is
unknown.
Therefore,
culturing
thawed
semen
samples
might
not
give
an
accurate
picture
of
the
bacteria
original
sample.
The
aim
this
study
was
to
assess
how
cryopreservation
and
storage
duration
influence
populations
survival
distinct
species.
Semen
were
collected
from
14
bulls,
diluted
equal
proportions
antibiotic-free
extender
transported
laboratory
at
6
°C
overnight.
Aliquots
cultured
within
24
h
after
collection
on
Plate
Count
Agar
calculate
number
bacteria,
blood
agar
plates
(5
%
bovine
blood)
identification
remaining
1:1
Brain
Heart
Infusion
(BHI)
broth
with
30
glycerol
stored
-80
°C.
frozen
quantification
as
described
fresh
semen,
13
days
isolated
re-cultured
agar,
incubated
one
day
37
before
by
Matrix
assisted
laser
desorption
ionization-time
flight
mass
spectrometry.
Total
counts
remained
consistent
across
cryopreserved
regardless
duration.
A
total
31
species
identified,
20
detected
samples,
16
present
storage,
18
observed
days.
Ten
persisted
all
time
points,
while
others
unique
a
specific
sampling
day,
including
nine
1,
two
6,
five
13.
These
findings
suggest
that
does
alter
overall
load,
individual
varies
depending
conditions.
Language: Английский
Sperm Selection by Colloid Centrifugation
Methods in molecular biology,
Journal Year:
2025,
Volume and Issue:
unknown, P. 249 - 265
Published: Jan. 1, 2025
Language: Английский
Reduced bacterial load in stallion semen by modified single layer centrifugation or sperm washing
Theriogenology,
Journal Year:
2023,
Volume and Issue:
216, P. 111 - 117
Published: Dec. 29, 2023
The
presence
of
bacteria
poses
a
significant
challenge
to
the
quality
stallion
semen
used
in
artificial
insemination.
bacterial
content
insemination
doses
arises
from
various
sources,
such
as
healthy
stallion,
environment,
and
collection
equipment,
is
implicated
fertility
problems
well
reduced
sperm
during
storage.
conventional
approach
adding
antibiotics
extenders
raises
concerns
about
antimicrobial
resistance
potential
negative
effects
on
characteristics,
may
not
be
effective
inhibiting
all
bacteria.
objective
this
study
was
determine
whether
an
innovative
alternative
antibiotic
usage
-
centrifugation
through
single
layer
low
density
colloid
(SLC)
could
reduce
load
semen,
compare
characteristics
samples
arising
procedure,
or
simple
extension
ejaculate
extender,
washing,
i.e.
extender
then
centrifuging
sample
allow
removal
most
seminal
plasma
extender.
Eighteen
were
collected
six
stallions.
split
extended
prior
washing
SLC,
received
no
further
treatment
other
than
extension.
After
preparation
aliquots
each
type
sent
for
bacteriological
examination;
remaining
stored
up
72
h,
with
daily
checks
quality.
SLC
outperformed
reduction,
effectively
removing
several
species.
follows:
16
±
6.7
×
10
Language: Английский