Goose Deoxycholic Acid Ameliorates Liver Injury in Laying Hens with Fatty Liver Hemorrhage Syndrome by Inhibiting the Inflammatory Response

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(1), P. 429 - 429

Published: Jan. 6, 2025

Fatty liver hemorrhagic syndrome (FLHS) in laying hens is a nutritional and metabolic disease involving enlargement, hepatic steatosis, hemorrhage as the primary symptoms. The prone to occur during peak period of hens, which has resulted significant economic losses hen breeding industry; however, specific pathogenesis FLHS remains unclear. Our group previous studies have shown that bile acid levels are significantly decreased development fatty targeted activation acid–related signaling pathways beneficial for preventing treating liver. In this study, we generated model by feeding high-energy, low-protein diet, with goose deoxycholic (CDCA) given an intervention. HE staining, fluorescence quantitative PCR, ELISA were used evaluate effects CDCA on pathological changes inflammatory responses results showed was reduced after treatment. Furthermore, fat vacuoles transaminase significantly. addition, expression M1-type macrophage markers polarization products reduced, pro-inflammatory regulatory factors related JAK-STAT pathway, LPS-TLR4-Myd88–NF-kB NLRP3 inflammasomes well. Expression M2-type increased significantly, did anti-inflammatory regulators pathway. These suggest ameliorates injury inhibiting resulting response, thereby promoting response.

Language: Английский

Impact of bacteroides uniformis on fatty liver hemorrhagic syndrome in dawu golden phoenix laying hens: modulation of gut microbiota and arachidonic acid metabolism

Frontiers in Microbiology, Journal Year: 2025, Volume and Issue: 16

Published: April 28, 2025

This study explored the impact of Bacteroides uniformis ( B. ) on fatty liver hemorrhagic syndrome (FLHS) induced by a high-energy and low-protein (HELP) diet in laying hens, mainly focusing hepatic lipid metabolism, gut microbiota, arachidonic acid (AA) metabolism. A total 120 Dawu Golden Phoenix hens (210-day-old) were randomly divided into four groups. The control group (CON) was fed standard received daily gavage PBS, while other groups with HELP to induce FLHS PBS (MOD), 1 × 10 9 CFU/ml (BUL), 11 (BUH) for 70 days. All administered ml gavage. Each had 6 replications 5 per replication. results showed that increased egg production rate feed conversion ratio decreased body weight, index, abdominal fat p < 0.05). treatment reduced accumulation reducing levels Triglyceride (TG), Total cholesterol (TC), low-density lipoprotein (LDL-C), alanine transaminases (ALT), aspartate (AST) serum significantly elevated high-density (HDL-C) indicated altered microbiota. Specifically, abundance higher, relative abundances Treponema, Helicobacter, Spirochaetota lower than those MOD Moreover, targeted metabolomic analysis supplementation 6-keto-PGF 1α AA levels, along thromboxane B 2 (TXB ), leukotriene D 4 (LTD 8-isoprostaglandin F 2α (8-iso-PGF 12S-hydroxyeicosatetraenoic (12S-HETE), 15S-hydroxyeicosatetraenoic (15S-HETE), 9-S-hydroxy-octadecadienoic (9S-HODE), 13-S-hydroxy-octadecadienoic (13S-HODE) In conclusion, oral intake can improve function, thereby helping ameliorate hens.

Language: Английский

Novel insights into the circadian modulation of lipid metabolism in chicken livers revealed by RNA sequencing and weighted gene co-expression network analysis

Poultry Science, Journal Year: 2024, Volume and Issue: 103(12), P. 104321 - 104321

Published: Sept. 17, 2024

Language: Английский