Discovery of novel treponemes associated with pododermatitis in elk ( Cervus canadensis ) DOI Creative Commons
Sushanta Deb, Margaret A. Wild,

Thomas LeClair

et al.

Applied and Environmental Microbiology, Journal Year: 2024, Volume and Issue: 90(6)

Published: May 14, 2024

ABSTRACT Pododermatitis, also known as treponeme-associated hoof disease (TAHD), presents a significant challenge to elk ( Cervus canadensis ) populations in the northwestern USA, with Treponema spp. consistently implicated lesion development. However, identifying species-specific strains from these lesions is hindered by its culture recalcitrance and limited genomic information. This study utilized shotgun sequencing, silico genome reconstruction, comparative genomics culture-independent approach identify metagenome-assembled genomes (MATGs) skin scraping samples collected captive experimentally challenged TAHD. The analysis revealed 10 new MATGs, 6 representing novel genomospecies associated pododermatitis 4 corresponding previously identified species —Treponema pedis phagedenis . Importantly, signatures of this were detected biopsy free-ranging diagnosed TAHD, indicating potential etiologic association. Comparative metabolic profiling MATGs against other showed distinct profile, suggesting host adaptation or geographic uniqueness newly genomospecies. discovery enhances our understanding pathogenesis lays foundation for development improved molecular surveillance tools monitor manage elk. IMPORTANCE play an important role elk; however, detection challenging due sequencing reconstruction pododermatitis. opens avenues develop diagnostic epidemiologic These findings significantly enhance landscape Treponemataceae consortium while offering valuable insights into etiology emerging populations.

Language: Английский

Genomic insights into Thermomonas hydrothermalis: potential applications in industrial biotechnology DOI
Songül Yaşar Yıldız

World Journal of Microbiology and Biotechnology, Journal Year: 2025, Volume and Issue: 41(2)

Published: Jan. 11, 2025

Language: Английский

Citations

0
First report on in-depth genome and comparative genome analysis of a metal-resistant bacterium Acinetobacter pittii S-30, isolated from environmental sample DOI Creative Commons
Rajnish Prakash Singh, Ayushi Sinha, Sushanta Deb

et al.

Frontiers in Microbiology, Journal Year: 2024, Volume and Issue: 15

Published: April 29, 2024

A newly isolated bacterium Acinetobacter pittii S-30 was recovered from waste-contaminated soil in Ranchi, India. The belongs to the ESKAPE organisms which represent major nosocomial pathogens that exhibit high antibiotic resistance. Furthermore, average nucleotide identity (ANI) analysis also showed its closest match (>95%) other A. genomes. isolate metal-resistant behavior and able survive up 5 mM of ZnSO 4 . Whole genome sequencing annotations revealed occurrence various genes involved stress protection, motility, metabolism aromatic compounds. Moreover, annotation identified gene clusters secondary metabolite production (biosynthetic clusters) such as arylpolyene, acinetobactin like NRP-metallophore, betalactone, hserlactone-NRPS cluster. metabolic potential based on cluster orthologous, Kyoto Encyclopedia Genes Genomes indicated a number related metal resistance, multiple drug-efflux systems etc., is relatively rare strains. Additionally, presence carbohydrate-active enzymes glycoside hydrolases (GHs), glycosyltransferases (GTs), associated with lignocellulose breakdown suggests strain has strong biomass degradation potential. an genetic diversity recombination strains performed understand population expansion hypothesis To our knowledge, this first report demonstrating detailed genomic characterization heavy belonging pittii. Therefore, could be good candidate for promotion plant growth biotechnological applications.

Language: Английский

Citations

3
Infant age inversely correlates with gut carriage of resistance genes, reflecting modifications in microbial carbohydrate metabolism during early life DOI Creative Commons
Xinming Xu,

Qingying Feng,

Tao Zhang

et al.

iMeta, Journal Year: 2024, Volume and Issue: 3(2)

Published: Jan. 31, 2024

Abstract The infant gut microbiome is increasingly recognized as a reservoir of antibiotic resistance genes, yet the assembly resistome in infants and its influencing factors remain largely unknown. We characterized 4132 metagenomes from 963 six countries 4285 genes were observed. inherent pattern healthy ( N = 272) could be distinguished by two stages: multicompound phase (Months 0–7) tetracycline‐mupirocin‐β‐lactam‐dominant 8–14). Microbial taxonomy explained 40.7% infants, with Escherichia (25.5%) harboring most genes. In further analysis all available 963), we found age was strongest influencer on negatively correlated overall during first 3 years p < 0.001). Using random‐forest approach, set 34 used to predict R 2 68.0%). Leveraging microbial host inference analyses, inferred age‐dependent result shifts microbiome, primarily driven changes taxa that disproportionately harbor across (e.g., coli more frequently harbored than other taxa). performed metagenomic functional profiling assembled genome analyses whose results indicate development carbohydrate metabolism, an increasing need for carbohydrate‐active enzymes Bacteroidota decreasing Pseudomonadota infancy. Importantly, observed increased acquired over time, which related horizontal gene transfer developing microbiome. summary, antimicrobial levels, reflecting composition shift likely changing metabolism early life.

Language: Английский

Citations

2
Discovery of novel treponemes associated with pododermatitis in elk ( Cervus canadensis ) DOI Creative Commons
Sushanta Deb, Margaret A. Wild,

Thomas LeClair

et al.

Applied and Environmental Microbiology, Journal Year: 2024, Volume and Issue: 90(6)

Published: May 14, 2024

ABSTRACT Pododermatitis, also known as treponeme-associated hoof disease (TAHD), presents a significant challenge to elk ( Cervus canadensis ) populations in the northwestern USA, with Treponema spp. consistently implicated lesion development. However, identifying species-specific strains from these lesions is hindered by its culture recalcitrance and limited genomic information. This study utilized shotgun sequencing, silico genome reconstruction, comparative genomics culture-independent approach identify metagenome-assembled genomes (MATGs) skin scraping samples collected captive experimentally challenged TAHD. The analysis revealed 10 new MATGs, 6 representing novel genomospecies associated pododermatitis 4 corresponding previously identified species —Treponema pedis phagedenis . Importantly, signatures of this were detected biopsy free-ranging diagnosed TAHD, indicating potential etiologic association. Comparative metabolic profiling MATGs against other showed distinct profile, suggesting host adaptation or geographic uniqueness newly genomospecies. discovery enhances our understanding pathogenesis lays foundation for development improved molecular surveillance tools monitor manage elk. IMPORTANCE play an important role elk; however, detection challenging due sequencing reconstruction pododermatitis. opens avenues develop diagnostic epidemiologic These findings significantly enhance landscape Treponemataceae consortium while offering valuable insights into etiology emerging populations.

Language: Английский

Citations

0