Journal of Industrial Microbiology & Biotechnology,
Journal Year:
2023,
Volume and Issue:
50(1)
Published: Jan. 1, 2023
Rhodotorula
toruloides
is
being
developed
for
the
use
in
industrial
biotechnology
processes
because
of
its
favorable
physiology.
This
includes
ability
to
produce
and
store
large
amounts
lipids
form
intracellular
lipid
bodies.
Nineteen
strains
were
characterized
mating
type,
ploidy,
robustness
growth,
accumulation
on
inhibitory
switchgrass
hydrolysate
(SGH).
Mating
type
was
determined
using
a
novel
polymerase
chain
reaction
(PCR)-based
assay,
which
validated
classical
microscopic
test.
Three
heterozygous
(A1/A2).
Ploidy
analysis
revealed
complex
pattern.
Two
triploid,
eight
haploid,
either
diploid
or
aneuploid.
A1/A2
compared
their
parents
growth
75%v/v
concentrated
SGH.
The
much
more
robust
than
parental
strains,
did
not
grow
had
extended
lag
times.
entire
set
evaluated
60%v/v
SGH
batch
cultures
kinetics
biomass
production.
Lipid
titers
2.33-9.40
g/L
with
median
6.12
g/L,
excluding
two
that
grow.
yields
0.032-0.131
(g/g)
contents
13.5-53.7%
(g/g).
Four
significantly
higher
contents.
One
these
among
highest
yield
this
study
(0.131
±
0.007
g/g),
has
been
previously
described
literature.The
yeast
used
oil
sugars
extracted
from
bioenergy
grass.
World Journal of Microbiology and Biotechnology,
Journal Year:
2025,
Volume and Issue:
41(3)
Published: March 1, 2025
Abstract
Oleaginous
yeasts
have
emerged
as
promising
microbial
cell
factories
for
lipid
production,
offering
sustainable
alternatives
to
traditional
sources
of
biodiesel
and
nutraceuticals.
In
this
study,
the
accumulation
potential
yeast
strains
isolated
from
two
freshwater
aquatic
ecosystems
in
Cali,
Colombia,
was
evaluated
identify
novel
candidates
biotechnological
applications.
A
total
56
were
tested
their
oleaginous
nature
using
a
gravimetric
assay
with
glucose
carbon
source.
Of
assessed
strains,
46.15%
exceeded
20%
yields
relative
dry
biomass.
Seven
selected
glycerol
source,
but
only
five
further
characterized
profiles.
Molecular
identification
revealed
diverse
species,
including
Aureobasidium
sp.,
Papiliotrema
rajashtanensis,
Rhodotorula
spp.,
Clavispora
lusitaniae
.
The
demonstrated
unique
profiles,
high
proportions
monounsaturated
polyunsaturated
fatty
acids,
such
oleic
acid
(C18:1)
linoleic
(C18:2).
particular,
sp.
accumulated
uncommon
acids
petroselinic
under
conditions
induced
by
glycerol.
This
acid,
which
has
double
bond
position
6,7
melting
point
33
°C,
highlights
its
an
alternative
margarine
well
precursor
sophorolipids,
estolide
esters,
soaps,
plastics.
exhibited
very
long-chain
docosadienoic
docosatrienoic
profile.
These
findings
underscore
value
lentic
systems
producers,
paving
way
innovations
biofuels,
nutraceuticals,
oleochemicals.
Applied Microbiology and Biotechnology,
Journal Year:
2025,
Volume and Issue:
109(1)
Published: March 29, 2025
Rhodotorula
toruloides
is
an
oleaginous
yeast
recognized
for
its
robustness
and
the
production
of
high
content
neutral
lipids.
Early
biochemical
studies
have
linked
ATP
citrate
lyase
(ACL),
phosphoketolase
(PK),
cytosolic
malic
enzyme
(cMAE)
with
de
novo
lipid
synthesis.
In
this
study,
we
discovered
that
upon
a
CRISPR/Cas9-mediated
knockout
ACL
gene,
in
R.
IFO0880
decreased
from
50
to
9%
dry
cell
weight
(DCW)
glucose
medium
caused
severe
growth
defects
(reduced
specific
rate,
changes
morphology).
xylose
medium,
43
38%
DCW.
However,
when
grown
on
acetate
as
sole
carbon
source,
45
20%
Significant
result
were
observed
all
substrates.
contrast,
PK
resulted
no
change
or
Knocking
out
cMAE
gene
increase
2.9%
DCW
23%
rate
glucose.
was
observed.
These
results
demonstrated
plays
crucial
role
synthesis
IFO0880,
opposed
pathway
cMAE,
whose
presence
some
conditions
even
disfavors
production.
provided
valuable
information
future
metabolic
engineering
toruloides.
•
fatty
acid
IFO0880.
Lipid
are
unchanged
knockout.
Cytosolic
does
not
play
significant
lipogenesis.
Journal of Fungi,
Journal Year:
2023,
Volume and Issue:
9(11), P. 1073 - 1073
Published: Nov. 2, 2023
The
presence
of
toxic
compounds
in
lignocellulosic
hydrolysates
(LCH)
is
among
the
main
barriers
affecting
efficiency
lignocellulose-based
fermentation
processes,
particular,
to
produce
biofuels,
hindering
production
intracellular
lipids
by
oleaginous
yeasts.
These
microbial
oils
are
promising
sustainable
alternatives
vegetable
for
biodiesel
production.
In
this
study,
we
explored
adaptive
laboratory
evolution
(ALE),
under
methanol-
and
high
glycerol
concentration-induced
selective
pressures,
improve
robustness
a
Rhodotorula
toruloides
strain,
previously
selected
from
sugar
beet
completely
using
major
C
(carbon)
sources
present.
An
evolved
multi-tolerant
not
only
methanol
but
four
inhibitors
present
LCH
(acetic
acid,
formic
hydroxymethylfurfural,
furfural)
was
isolated
mechanisms
underlying
such
multi-tolerance
were
examined,
at
cellular
envelope
level.
Results
indicate
that
strain
has
cell
wall
less
susceptible
zymolyase
decreased
permeability,
based
on
propidium
iodide
fluorescent
probe,
absence
or
those
inhibitors.
improved
performance
lipid
synthetic
hydrolysate
medium,
supplemented
with
inhibitors,
confirmed.
Heliyon,
Journal Year:
2024,
Volume and Issue:
10(22), P. e39742 - e39742
Published: Oct. 24, 2024
The
transition
to
electric
vehicles
(EVs)
calls
for
sustainable
advancements
in
automotive
lubricants,
as
traditional
fossil-fuel-based
products
pose
environmental
challenges.
Palm
oil-based
biolubricants
enriched
with
nanoparticles
present
a
promising
eco-friendly
alternative
that
meets
the
thermal
and
tribological
demands
of
EVs.
This
paper
aims
analyze
development
nanoparticle-enriched
palm
biolubricants,
aimed
at
improving
sustainability
performance
vehicle
(EV)
lubrication
systems.
critical
findings
highlight
integrating
such
graphene,
titanium
dioxide,
aluminum
oxide
into
lubricants
significantly
enhances
their
properties.
These
enhancements
include
26.21%-34%
reduction
coefficient
friction
(COF),
12.99%-30%
wear,
improved
stability.
study
found
outperformed
options
terms
wear
under
high-temperature
pressure
conditions,
supported
by
regression
analysis.
demonstrates
offer
viable
conventional
lowering
impact
reducing
greenhouse
gas
emissions
energy
consumption.
innovation
has
significant
implications
both
environment
industry,
offering
solution
reduces
dependency
on
fossil
fuels,
EV
efficiency,
aligns
global
goals.
Besides,
this
discusses
drawbacks
future
studies
direction.
