Early life imidacloprid and copper exposure affects the gut microbiome, metabolism, and learning ability of honey bees (Apis mellifera).

Environmental Research, Journal Year: 2025, Volume and Issue: unknown, P. 121134 - 121134

Published: Feb. 1, 2025

Language: Английский

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A synthesis and quantification method for endogenous metabolites dimethylguanidino valeric acid

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: April 1, 2025

Dimethylguanidino valeric acid (DMGV) is a group of endogenous metabolites derived from arginine-containing proteins and associated with several metabolic disorders. Latest studies have identified the stereoisomers DMGV: asymmetric dimethylguanidino (ADGV) symmetric (SDGV), which are dimethylarginine (ADMA) (SDMA), respectively. However, lack commercial standards has hampered research into molecular mechanisms DMGV its potential clinical application as biomarkers. Reported chemical synthesis methods low yields require specialized skills apparatus. Therefore, we aimed to develop practical efficient method synthesize determine their precise concentration profile in healthy subjects. A novel biocatalytic for synthesizing using alanine-glyoxylate aminotransferase 2 (AGXT2) was developed. metabolite panel including eight DMGV-related also established human plasma samples HPLC-MS/MS, performance comprehensively evaluated, especially terms quantitative sensitivity, precision accuracy. Compared reported methods, approach demonstrates superior yield, conversion rate, product purity, while being easy implement biological laboratories. The quantification been well validated successfully applied measure contents these metabolites, ADGV individuals. To sum up, this study provides an determines levels These findings will undoubtedly promote future mechanistic applications.

Language: Английский

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Fluorogenic, Subsingle-Turnover Monitoring of Enzymatic Reactions Involving NAD(P)H Provides a Generalized Platform for Directed Ultrahigh-Throughput Evolution of Biocatalysts in Microdroplets

Journal of the American Chemical Society, Journal Year: 2025, Volume and Issue: unknown

Published: March 24, 2025

Enzyme engineering and discovery are crucial for a sustainable future bioeconomy. Harvesting new biocatalysts from large libraries through directed evolution or functional metagenomics requires accessible, rapid assays. Ultrahigh-throughput screening formats often require optical readouts, leading to the use of model substrates that may misreport target activity necessitate bespoke synthesis. This is particular challenge when glycosyl hydrolases, which leverage molecular recognition beyond glycosidic bond, so complex chemical synthesis would have be deployed build fluoro- chromogenic substrate. In contrast, coupled assays represent modular "plug-and-play" system: any enzyme–substrate pairing can investigated, provided reaction produce common intermediate links catalytic detection cascade readout. Here, we establish producing fluorescent readout in response NAD(P)H via glutathione reductase subsequent thiol-mediated uncaging reaction, with low nanomolar limit plates. Further scaling down microfluidic droplet possible: fluorophore leakage-free report 3 orders magnitude-improved sensitivity compared absorbance-based systems, resolution 361,000 product molecules per droplet. Our approach enables nonfluorogenic droplet-based enrichments, applicability hydrolases imine reductases (IREDs). To demonstrate assay's readiness combinatorial experiments, one round was performed select glycosidase processing natural substrate, beechwood xylan, improved kinetic parameters pool >106 mutagenized sequences.

Language: Английский

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Determination of the Composition and Monosaccharide Content of Atractylodes Polysaccharides Using Pre-column Derivatization and a Quantitative Analysis of Multicomponents by a Single Marker Method

Journal of Chromatography Open, Journal Year: 2025, Volume and Issue: unknown, P. 100224 - 100224

Published: May 1, 2025

Language: Английский

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Discovering Phlebia acerina for efficient degradation of bisphenol S and insights into its degradation mechanism

Bioresource Technology, Journal Year: 2024, Volume and Issue: 418, P. 131912 - 131912

Published: Nov. 28, 2024

Language: Английский

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Bioactive Phenylpropanoid Glycosides, Dimers, and Heterodimers from the Bark of Cinnamomum cassia (L.) J.Presl

Journal of Agricultural and Food Chemistry, Journal Year: 2024, Volume and Issue: 72(29), P. 16263 - 16275

Published: July 2, 2024

Six new phenylpropanoid glycosides (

Language: Английский

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