Metabolic Engineering and Adaptive Evolution of Escherichia coli for Enhanced Conversion of D‑Xylose to D‐Glucaric Acid Mediated by Methanol DOI Open Access
Weixiang Chen,

Lingjie Zheng,

Xuan Luo

et al.

Biotechnology and Bioengineering, Journal Year: 2025, Volume and Issue: unknown

Published: March 18, 2025

ABSTRACT d ‐Glucaric acid is a value‐added dicarboxylic that can be utilized in the chemical, food, and pharmaceutical industries. Due to complex process environmental pollution associated with chemical production of ‐glucaric acid, bioconversion for its synthesis has garnered increasing attention recent years. In this study, novel cell factory was developed efficient using ‐xylose methanol. Mdh, Hps, Phi, Miox, Ino1, Suhb, Udh were first co‐expressed E. coli JM109 construct pathway. The deletion FrmRAB, RpiA, PfkA, PfkB then performed block or weaken endogenous competitive pathways. Next, adaptive evolution carried out improve growth substrate utilization. With purpose further product titer, NusA tag myo‐inositol biosensor introduced into engineered enhance Miox expression. After medium optimization fermentation control, 3.0 g/L finally obtained fed‐batch modified Terrific Broth medium.

Language: Английский

Metabolic Engineering and Adaptive Evolution of Escherichia coli for Enhanced Conversion of D‑Xylose to D‐Glucaric Acid Mediated by Methanol DOI Open Access
Weixiang Chen,

Lingjie Zheng,

Xuan Luo

et al.

Biotechnology and Bioengineering, Journal Year: 2025, Volume and Issue: unknown

Published: March 18, 2025

ABSTRACT d ‐Glucaric acid is a value‐added dicarboxylic that can be utilized in the chemical, food, and pharmaceutical industries. Due to complex process environmental pollution associated with chemical production of ‐glucaric acid, bioconversion for its synthesis has garnered increasing attention recent years. In this study, novel cell factory was developed efficient using ‐xylose methanol. Mdh, Hps, Phi, Miox, Ino1, Suhb, Udh were first co‐expressed E. coli JM109 construct pathway. The deletion FrmRAB, RpiA, PfkA, PfkB then performed block or weaken endogenous competitive pathways. Next, adaptive evolution carried out improve growth substrate utilization. With purpose further product titer, NusA tag myo‐inositol biosensor introduced into engineered enhance Miox expression. After medium optimization fermentation control, 3.0 g/L finally obtained fed‐batch modified Terrific Broth medium.

Language: Английский

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