Quick Identification of Genetic Provenances and Best Practice Woodland Management of Chinese Pine (Pinus tabuliformis) DOI Creative Commons

Hanhan Xia,

Minyi Yang,

Feicui Yang

et al.

Research Square (Research Square), Journal Year: 2021, Volume and Issue: unknown

Published: June 25, 2021

Abstract Chinese pine ( Pinus tabuliformis ) is one of the most widespread, ecologically and economically important tree species in North China. In this study, we analyzed compared genetic diversity population structure 158 individuals from 17 populations P. , by group III, a new mitochondrial marker system nad1-2, nad4-3, nad5-1 nad7-1 with two other groups systems. contrast to conservation evolution sequence, shows as extremely high polymorphism 25, whose haplotypes are more than four times I nad1-2 nad4-3 nad5-1) 8 haplotypes. Although II, (19 haplotypes), showed resolution provenance identification accurate for detection specific like HL, WT NS powerful differentiate between GD SS. The results suggested that GBS (genotype seqencing). It much available will be great help molecular assisted breeding . This study make theoretical foundation following studies on evaluation, cultivation germplasm management aid breeding, biodiversity programs forest species.

Language: Английский

Breeding and genetics of disease resistance in temperate fruit trees: challenges and new opportunities DOI
Awais Khan, Schuyler S. Korban

Theoretical and Applied Genetics, Journal Year: 2022, Volume and Issue: 135(11), P. 3961 - 3985

Published: April 20, 2022

Language: Английский

Citations

50
Genomic Approaches for Improvement of Tropical Fruits: Fruit Quality, Shelf Life and Nutrient Content DOI Open Access

Malarvizhi Mathiazhagan,

Bhavya Chidambara,

R. H. Laxman

et al.

Genes, Journal Year: 2021, Volume and Issue: 12(12), P. 1881 - 1881

Published: Nov. 25, 2021

The breeding of tropical fruit trees for improving traits is complicated, due to the long juvenile phase, generation cycle, parthenocarpy, polyploidy, polyembryony, heterozygosity and biotic abiotic factors, as well a lack good genomic resources. Many molecular techniques have recently evolved assist hasten conventional efforts. Molecular markers linked development quality such shape, size, texture, aroma, peel pulp colour were identified in crops, facilitating Marker-assisted (MAB). An increase availability genome sequences fruits further aided discovery SNP variants/Indels, QTLs genes that can ascertain genetic determinants characters. Through multi-omics approaches genomics, transcriptomics, metabolomics proteomics, identification quantification transcripts, including non-coding RNAs, involved sugar metabolism, ripening, shelf life, stress impacts made possible. Utilizing assisted methods wide association (GWAS), selection (GS) modifications using CRISPR/Cas9 transgenics has paved way studying gene function developing cultivars with desirable by overcoming cycles. Such comprehensive related characters their applications strategies crop improvement are reviewed, discussed presented here.

Language: Английский

Citations

43
Phased telomere-to-telomere reference genome and pangenome reveal an expansion of resistance genes during apple domestication DOI Creative Commons
Ying Su,

X Yang,

Y. Wang

et al.

PLANT PHYSIOLOGY, Journal Year: 2024, Volume and Issue: 195(4), P. 2799 - 2814

Published: May 14, 2024

Abstract The cultivated apple (Malus domestica Borkh.) is a cross-pollinated perennial fruit tree of great economic importance. Earlier versions reference genomes were unphased, fragmented, and lacked comprehensive insights into the apple's highly heterozygous genome, which impeded advances in genetic studies breeding programs. In this study, we assembled haplotype-resolved telomere-to-telomere (T2T) genome for diploid cultivar Golden Delicious. Subsequently, constructed pangenome based on 12 assemblies from wild species to investigate dynamic changes functional genes. Our results revealed gene gain loss events during domestication. Compared with species, more families significantly enriched oxidative phosphorylation, pentose metabolic process, responses salt, abscisic acid biosynthesis process. analyses also demonstrated higher prevalence different types resistance analogs (RGAs) cultivars than their relatives, partially attributed segmental tandem duplication certain RGAs classes. Structural variations, mainly deletions insertions, have affected presence absence TIR-NB-ARC-LRR, NB-ARC-LRR, CC-NB-ARC-LRR Additionally, hybridization/introgression has contributed expansion genes domesticated apples. T2T provide important resources apples, emphasizing need study evolutionary mechanisms breeding.

Language: Английский

Citations

8
RosBREED: bridging the chasm between discovery and application to enable DNA-informed breeding in rosaceous crops DOI Creative Commons

Amy Iezzoni,

Jim McFerson,

James J. Luby

et al.

Horticulture Research, Journal Year: 2020, Volume and Issue: 7(1)

Published: Nov. 1, 2020

Abstract The Rosaceae crop family (including almond, apple, apricot, blackberry, peach, pear, plum, raspberry, rose, strawberry, sweet cherry, and sour cherry) provides vital contributions to human well-being is economically significant across the U.S. In 2003, industry stakeholder initiatives prioritized utilization of genomics, genetics, breeding develop new cultivars exhibiting both disease resistance superior horticultural quality. However, rosaceous breeders lacked certain knowledge tools fully implement DNA-informed breeding—a “chasm” existed between existing genomics genetic information application this in breeding. RosBREED project (“Ros” signifying a community initiative, “BREED”, indicating core focus on programs), addressed challenge through comprehensive coordinated 10-year effort funded by USDA-NIFA Specialty Crop Research Initiative. was designed enable routine modern genetics technologies programs, thereby enhancing their efficiency effectiveness delivering with producer-required resistances market-essential This review presents synopsis approach, deliverables, impacts RosBREED, highlighting synergistic global collaborations future needs. Enabling developed are described, including genome-wide scanning platforms DNA diagnostic tests. Examples use participants presented for all stages, pre-breeding resistance, parental seedling selection, elite selection advancement. chasm now bridged, accelerating improvement.

Language: Английский

Citations

46
Genome-wide associations within diverse wild apple germplasm for postharvest blue mold resistance to Penicillium expansum DOI Creative Commons
Lauren Whitt, John S. Bennett, Tamara D. Collum

et al.

Postharvest Biology and Technology, Journal Year: 2025, Volume and Issue: 225, P. 113513 - 113513

Published: March 18, 2025

Language: Английский

Citations

0
Application of new breeding techniques in fruit trees DOI Open Access
Manuela Campa, Simón Miranda, Concetta Licciardello

et al.

PLANT PHYSIOLOGY, Journal Year: 2023, Volume and Issue: unknown

Published: July 3, 2023

Abstract Climate change and rapid adaption of invasive pathogens pose a constant pressure on the fruit industry to develop improved varieties. Aiming accelerate development better-adapted cultivars, new breeding techniques have emerged as promising alternative meet demand growing global population. Accelerated breeding, cisgenesis, CRISPR/Cas genome editing hold significant potential for crop trait improvement proven be useful in several plant species. This review focuses successful application these technologies trees confer pathogen resistance tolerance abiotic stress improve quality traits. In addition, we optimization diversification tools applied trees, such multiplexing, CRISPR/Cas-mediated base site-specific recombination systems. Advances protoplast regeneration delivery techniques, including use nanoparticles viral-derived replicons, are described obtention exogenous DNA-free tree The regulatory landscape broader social acceptability cisgenesis also discussed. Altogether, this provides an overview versatility applications improvement, well current challenges that deserve attention further implementation techniques.

Language: Английский

Citations

9
Recent Developments and Strategies for the Application of Agrobacterium-Mediated Transformation of Apple Malus × domestica Borkh DOI Creative Commons
Susan Schröpfer, Janne Lempe, Ofere Francis Emeriewen

et al.

Frontiers in Plant Science, Journal Year: 2022, Volume and Issue: 13

Published: June 30, 2022

Genetic transformation has become an important tool in plant genome research over the last three decades. This applies not only to model plants such as Arabidopsis thaliana but also increasingly cultivated plants, where establishment of methods could still pose many problems. One is apple ( Malus spp.), most fruit temperate climate zone. Although genetic using Agrobacterium tumefaciens been possible since 1989, a few groups worldwide have successfully applied this technology, and efficiency remains poor. Nevertheless, there some developments, especially recent years, which allowed for expansion toolbox breeders breeding researchers. review article attempts summarize developments -mediated strategies apple. In addition use different tissues media transformation, agroinfiltration, well pre-transformation with Baby boom transcription factor are notable successes that improved Further, we highlight targeted gene silencing applications. Besides classical RNAi-based by stable hairpin constructs, optimized protocols virus-induced (VIGS) artificial micro RNAs (amiRNAs) emerged powerful technologies genes interest. Success achieved establishing editing (GE). For example, it was recently first time generate homohistont GE line into biallelic mutation specifically inserted target gene. these methods, primarily aimed at increasing efficiency, improving precision modification reducing required, discussed genetically modified used purposes. particular, current state rapid crop cycle system its applications will be presented.

Language: Английский

Citations

14
Field study of the fire‐blight‐resistant cisgenic apple line C44.4.146 DOI Creative Commons
Ina Schlathölter, Giovanni A. L. Broggini, Sebastian Streb

et al.

The Plant Journal, Journal Year: 2023, Volume and Issue: 113(6), P. 1160 - 1175

Published: Jan. 7, 2023

SUMMARY Cisgenesis, the genetic modification of a plant with genes from sexually compatible plant, was used to confer fire blight resistance cultivar ‘Gala Galaxy’ by amendment gene FB_MR5 , resulting in line C44.4.146. To verify whether cisgenesis changed other tree‐, flower‐ or fruit‐related traits, 5‐year field trial conducted trees C44.4.146 and multiple control genotypes, including members ‘Gala’ sports group. None 44 investigated traits significantly differed between at least one genotypes all observation years. However, fruits its wild‐type tissue culture were paler color than that had not undergone culture. There no significant consistently detected difference fruit flesh peel metabolome compared genotypes. Finally, disease confirmed also when pathogen inoculated through flowers. We conclude use did have unintended effects, vitro establishment greater effect on properties generation applying cisgenesis.

Language: Английский

Citations

5
Deducing genotypes for loci of interest from SNP array data via haplotype sharing, demonstrated for apple and cherry DOI Creative Commons
Alexander Schaller, Stijn Vanderzande, Cameron Peace

et al.

PLoS ONE, Journal Year: 2023, Volume and Issue: 18(2), P. e0272888 - e0272888

Published: Feb. 7, 2023

Breeders, collection curators, and other germplasm users require genetic information, both genome-wide locus-specific, to effectively manage their genetically diverse plant material. SNP arrays have become the preferred platform provide profiles for elite could also locus-specific genotypic information. However, information loci of interest such as those within PCR-based DNA fingerprinting panels trait-predictive tests is not readily extracted from array data, thus creating a disconnect between historic new data sets. This study aimed establish method deducing genotypes at associated haplotypes, demonstrated two fruit crops three locus types: quantitative trait Ma Ma3 acidity in apple, apple microsatellite marker GD12, Mendelian R f sweet cherry color. Using phased an 8K 6K array, unique haplotypes spanning each target were with alleles important breeding parents. These compared via identity-by-descent (IBD) or identity-by-state (IBS) present U.S. programs deduce this germplasm. While IBD segments confidently tracked through pedigrees, confidence allele identity among IBS used shared length threshold. At least one per was deduced 64–93% 181 individuals. Successful validation GD12 reported newly obtained genotypes. Our approach can efficiently merge expand sets, missing identifying errors, appropriate any crop especially where linkage disequilibrium high. Locus-specific expected enhance management resources.

Language: Английский

Citations

4
Delivering the goods: Fungal secretion modulates virulence during host–pathogen interactions DOI Creative Commons
Michael Bradshaw, Holly P. Bartholomew, Jorge M. Fonseca

et al.

Fungal Biology Reviews, Journal Year: 2021, Volume and Issue: 36, P. 76 - 86

Published: April 21, 2021

Language: Английский

Citations

10