Metabolomics Provides Novel Insights into Epilepsy Diagnosis and Treatment: A Review

Neurochemical Research, Journal Year: 2022, Volume and Issue: 47(4), P. 844 - 859

Published: Jan. 24, 2022

Language: Английский

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Multifaceted targets of cannabidiol in epilepsy: Modulating glutamate signaling and beyond

Computers in Biology and Medicine, Journal Year: 2024, Volume and Issue: 179, P. 108898 - 108898

Published: July 23, 2024

Language: Английский

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Integrating metabolomics and lipidomics revealed a decrease in plasma fatty acids but an increase in triglycerides in children with drug‐refractory epilepsy

Epilepsia Open, Journal Year: 2023, Volume and Issue: 8(2), P. 466 - 478

Published: Feb. 20, 2023

The drug-refractory epilepsy (DRE) in children is commonly observed but the underlying mechanisms remain elusive. We examined whether fatty acids (FAs) and lipids are potentially associated with pharmacoresistance to valproic acid (VPA) therapy.This single-center, retrospective cohort study was conducted using data from pediatric patients collected between May 2019 December at Children's Hospital of Nanjing Medical University. Ninety plasma samples 53 responders VPA monotherapy (RE group) 37 non-responders polytherapy (NR were collected. Non-targeted metabolomics lipidomics analysis for those performed compare potential differences small metabolites two groups. Plasma passing threshold variable importance projection value >1, fold change >1.2 or <0.8, p-value <0.05 regarded as statistically different substances.A total 204 433 comprising 16 lipid subclasses identified. well-established partial least squares-discriminant (PLS-DA) revealed a good separation RE NR group. FAs glycerophospholipids status significantly decreased group, their triglycerides (TG) levels increased. trend TG routine laboratory tests line analysis. Meanwhile, cases group characterized by level citric L-thyroxine, an increased glucose 2-oxoglutarate. top enriched metabolic pathways involved DRE condition biosynthesis unsaturated linoleic metabolism.The results this suggested association metabolism medically intractable epilepsy. Such novel findings might propose mechanism linked energy metabolism. Ketogenic supplementation therefore be high-priority strategies management.

Language: Английский

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Identification, Clinical Values, and Prospective Pathway Signaling of Lipid Metabolism Genes in Epilepsy and AED Treatment

Molecular Neurobiology, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 15, 2025

Language: Английский

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Mass Spectrometry Imaging Deciphers Dysregulated Lipid Metabolism in the Human Hippocampus Affected by Temporal Lobe Epilepsy

ACS Chemical Neuroscience, Journal Year: 2021, Volume and Issue: 12(21), P. 4187 - 4194

Published: Oct. 16, 2021

Temporal lobe epilepsy (TLE) is the most prevalent form of human epilepsy, often accompanied by neurodegeneration in hippocampus. Like other neurological diseases, TLE expected to disrupt lipid homeostasis. However, architecture brain relatively understudied, and molecular mechanism epileptogenesis poorly understood. We performed desorption electrospray ionization mass spectrometry imaging 39 fresh frozen surgical specimens hippocampus investigate profiles with hippocampal sclerosis (n = 14) control (non-TLE; n 25) groups. In contrast several previous studies on animal models we report reduced expression various important lipids, notably phosphatidylcholine (PC) phosphatidylethanolamine (PE), addition, metabolic pathway analysis suggested possible dysregulation Kennedy TLE, resulting striking reductions PC PE levels. This revelation opens up opportunities further associated mechanisms therapeutic targets for TLE.

Language: Английский

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Lipid metabolism: Novel approaches for managing idiopathic epilepsy

Neuropeptides, Journal Year: 2024, Volume and Issue: 108, P. 102475 - 102475

Published: Sept. 30, 2024

Language: Английский

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Pathology-specific lipid alterations with triacylglycerol as a potential biomarker in Focal cortical dysplasia (FCD) and Mesial Temporal Lobe Epilepsy with Hippocampal Sclerosis (MTLE-HS)

Neuroscience, Journal Year: 2024, Volume and Issue: 566, P. 72 - 86

Published: Dec. 22, 2024

Language: Английский

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Hippocampal metabolic profile during epileptogenesis in the pilocarpine model of epilepsy

Biomedical Chromatography, Journal Year: 2023, Volume and Issue: 38(4)

Published: Dec. 28, 2023

Abstract Temporal lobe epilepsy (TLE) is a common form of refractory in adulthood. The metabolic profile epileptogenesis still poorly investigated. Elucidation such using animal models could help identify new metabolites and pathways involved the mechanisms process. In this study, we evaluated during periods. Using pilocarpine model epilepsy, analyzed global hippocampal extracts by untargeted metabolomics based on ultra‐performance liquid chromatography–high‐resolution mass spectrometry, at three time points (3 h, 1 week, 2 weeks) after status epilepticus ( SE ) induction. We demonstrated that periods presented different profiles, including alterations amino acids lipid metabolism. Six putative (tryptophan, N ‐acetylornithine, ‐acetyl‐ L ‐aspartate, glutamine, adenosine, cholesterol) showed significant levels compared to their respective controls. These be associated with imbalance neurotransmitters, mitochondrial dysfunction, cell loss observed both epilepsy. With these findings, provided an overview profiles stages investigate as predictive tools

Language: Английский

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A companion to the preclinical common data elements for proteomics, lipidomics, and metabolomics data in rodent epilepsy models. A report of the TASK3‐WG4 omics working group of the ILAE/AES joint translational TASK force

Epilepsia Open, Journal Year: 2022, Volume and Issue: unknown

Published: Oct. 19, 2022

The International League Against Epilepsy/American Epilepsy Society (ILAE/AES) Joint Translational Task Force established the TASK3 working groups to create common data elements (CDEs) for various preclinical epilepsy research disciplines. This is second in a two-part series of omics papers, with other including genomics, transcriptomics, and epigenomics. aim CDEs was improve standardization experimental designs across range research-related methods. We have generated CDE tables key parameters case report forms (CRFs) containing essential contents study protocols proteomics, lipidomics, metabolomics samples from rodent models people epilepsy. discuss important that need be considered methodologies, providing rationale should documented.

Language: Английский

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Untargeted metabolomics profiling in pediatric patients and adult populations indicates a connection between lipid imbalance and epilepsy

medRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: March 30, 2023

Abstract Introduction Epilepsy is a common central nervous system disorder characterized by abnormal brain electrical activity. We aimed to compare the metabolic profiles of plasma from patients with epilepsy across different etiologies, seizure frequency, type, and patient age try identify disrupted pathways. Material methods used data three separate cohorts. The first cohort (PED-C) consisted 31 pediatric suspicion genetic unclear etiology; second (AD-C) 250 adults Estonian Biobank (EstBB), third 583 ≥ 69 years EstBB (ELD-C). compared untargeted metabolomics lipidomics between individuals without in each cohort. Results In PED-C, significant alterations (p-value <0.05) were detected sixteen glycerophosphatidylcholines (GPC), dimethylglycine eicosanedioate (C20-DC). AD-C, nine significantly altered metabolites found, mainly triacylglycerides (TAG), which are also precursors GPC synthesis pathway. ELD-C, changes twenty including multiple TAGs observed profile participants previously diagnosed epilepsy. Pathway analysis revealed that among differ epilepsy-positive epilepsy-negative lipid superpathway (p = 3.2*10-4) phosphatidylcholine 9.3*10-8) lysophospholipid 5.9*10-3) subpathways statistically overrepresented. Analogously, triacylglyceride subclass turned out be overrepresented 8.5*10-5) 1.4*10-2). presented p-values FDR-corrected. Conclusion Our results suggest cell membrane fluidity may have role mechanism epilepsy, balance indicate However, further studies needed evaluate whether could prove helpful diagnosing earlier.

Language: Английский

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